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The aim of the present study was to evaluate, using the microscope and computer image analysis system MultiScan, the effects of Cu (0.2 mg·dm -3) and Cd (0.2 mg·dm -3) on swimbladder inflation by common carp (Cyprinus carpio L.) larvae under laboratory conditions. The fish were exposed to metals for 30 days from hatching. The results indicate that heavy metals considerably inhibited inflation of the posterior and anterior chamber of swimbladder. Metals affected inflation of each swimbladder chamber in a different way. They reduced the rate of inflation of first (posterior) chamber, delayed the beginning of inflation of the second (anterior) chamber, and inhibited its growth. Metal exposure resulted in differences among the larvae: some of them inflated the anterior chamber, and the others failed to inflate it.
The aim of this study was to describe the development of the swim bladder of pikeperch Sander lucioperca (L.) under conditions of intensive culture and to determine when the pneumatic duct regresses. The development of the swim bladder was observed over a 30-day period of larvae culture in a recirculation system. The swim bladder and the pneumatic duct were clearly visible in histological cross sections of 4-day-old larvae. On day 6 after hatching significant changes occurred in the microscopic structure of the swim bladder, e.g. the gas gland and oval primordia appeared. The pneumatic duct gradually atrophied in the larvae with inflated swim bladders between days 11 and 13, while it remained open until day 24-26 after hatching in fish with noninflated swim bladders. The swim bladders of the latter group were irregular in shape and hyperplasia of the epithelium and connective tissues and the presence of macrophags were noted. Pikeperch larvae with noninflated swim bladders also suffered from lordosis and slower growth rates.
The morphogenesis of the digestive tract and swim bladder of ide Leuciscus idus (L.) was analyzed using histopathological methods. At the moment of hatching, ide larvae are more advanced in their development than the larvae of other teleost fish species. Endo-exogenous nutrition began on day 2 post hatch. At this stage of development, the intestine was lined with a single-layered cylindrical epithelium, and both liver and pancreas began to function. Signs of digestion and enterocyte protein and lipid absorption were observed on day 6 of development. The mucus cells in the mouth, esophagus and gullet produced neutral and acidic mucins. Intestinal cuboid cells appeared on the fourth day and only began secreting acidic carboxyl and sulfate mucins. The differentiation of the air duct and the posterior chamber of the swim bladder was observed on the day of hatching, and the bladder inflated after 24 hours. The anterior chamber began to form on day 9, and it inflated with gas between days 23 and 25 of larval development.
Scanning electron microscopy studies of nematode specimens of Cystidicola farionis Fischer, 1798, a swimbladder parasite mainly of salmonids, made it possible to describe in detail the cephalic structure as well as some additional morphological features of this widely distributed nematode and, accordingly, to correct the generic diagnosis of Cystidicola Fischer, 1798. Similarly to species of the most related genera Comephoronema Layman, 1933 and Salvelinema Trofimenko, 1962, the males of C. farionis (a type species of the genus) possess well-developed ventral precloacal ridges (area rugosa). As shown by this study, the genus Cystidicola differs from Comephoronema and Salvelinema mainly in the structure of the mouth, particularly in the presence of four well-demarcated labia, large circumoral teeth, and in the character of pseudolabia.
The greatest losses in central and eastern European carp culture are caused by the high mortality of juveniles usually during the first three to four weeks after pond stocking in late spring. The pronounced weather fluctuations of this period, including drastic falls in temperature, endanger the survival of this warm-water fish. By quantifying the effect of considerable temperature decreases on the survival of juvenile carp, logarithmic increases in carp survival rate were apparent as favourable thermal conditions extended from 1 to 40 days. Simultaneously, a decrease in carp swim bladder inflammation was observed. The trend toward increasing frequency and amplitude of weather fluctuations might intensify this problem.
The study focused on the eel (length and weight averaging 23.1 cm and 13.7 g, respectively) ascending Pomeranian rivers in summers of 1999-2003. The swim bladder was examined in 322 individuals, the intestine being examined in 272 fish. Anguillicola crassus was present in the swim bladder of 184 fish. Infection prevalence varied between the rivers, the highest prevalence being recorded in the eel caught in the Radew (65.6%) and the lowest in the Wieprza (41.7%). The intensity of infection was generally low, the mean values varying from 2. l in the Rega to 1.3 in the Wieprza. The coefficient of infection was low (0,11 to 0,12). Spinitectus-inermis was present, with low intensity, in the intestine of 18 fish (6.6%). The nematode had not been recorded in the eel in Poland for 70 years. In addition, the intestine of the eel under study was found to house Raphidascaris acus and Paraquimperia tennerima, each present as a single larva. This is the first record of the latter in Poland. The infection of eel from the Radew river that stayed in the fresh water for a longer time was higher than that of the eel found in the Rega and Wieprza.
An experiment was conducted to test the effect of the infection by the swimbladder nematode Anguillicola crassus on the survival of the European eel Anguilla anguilla when exposed to hypoxic conditions. Forty-four wild caught and naturally infected eels were placed in an aquarium filled with water from the fishing site (Vaccarès Lagoon, French Mediterranean coast). In this confined environment (271), under decreasing oxygen resources, the first eel death occurred after 45 h (O2 = 0.98 mg l−1) and the last one after 96 h (O2 = 0.48 mg l−1). After dissection and parasite examination, analyses revealed significant negative correlations between the time to death and various parameters of parasite pressure (e.g., number of lumen worms, parasite mass, health state of the infected organ). It was shown that the severity of damage to the swimbladder rather than the count of living parasites was the major contributing factor in explaining the variation in time to death. These semi-experimental data demonstrating an increased eel mortality rate under severe oxygen stress are discussed for their relevance under field conditions, especially during hot summer months.
Carp were cultivated (from day 3 to 30 after hatching) in water with a nitrite concentration of 7 mg NO₂-N dm³. The results of histological studies confirmed pathological changes in the throat, gills, heart, blood, swim bladder, liver and kidneys. These changes mainly had an adverse impact on respiration and growth. The total length and weight of fish from water with nitrites was lower by 30 and 75%, respectively, and their survival rate was 62% (97% in the control group).
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