Morphological and chemical diversity of three populations of sweet woodruff (Galium odo- ratum L.) was investigated in situ and ex situ. The herb was collected in two stages of plants development: in full blooming and after blooming. Content of coumaric compounds, flavonoids and phenolic acids were determined. Investigated populations didn't differ significantly as to the morphological features of plants but they did as to the content of the biologically active compounds.The content of investigated pharmacologically active compounds depended both on the location of population and the growth phases of plants.