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The study was carried out in 2002 and 2003. The experimental material was basil plants cultivated in the field for a bunch harvest, using for plant covering perforated foil and polypropylene unwoven cloth. The control object was basil grown without covering. The covers tested in the experiment had a significant influence on the yielding of basil cultivated for a bunch harvest. The highest yield was obtained when polypropylene unwoven cloth was used. Basil plants grown under polypropylene unwoven cloth were the highest (18.2 cm), had the biggest diameter (13.4 cm) and the largest leaves (5.9 cm long and 3.3 cm wide). Using covers (perforated foil and polypropylene unwoven cloth) caused a decrease of dry matter content in the yield of basil, in comparison with the plants grown without covering.The covers used in the study caused a decrease of nitrogen, potassium, calcium and magnesium in the basil yield, but did not decrease phosphorus and sodium content.
It was demonstrated that the content of essential oil in sweet basil depends on the level of nitrogen nutrition and on the term of herb harvest. The greatest content of the essential oil was found in herb harvested at the beginning of blooming using 0.9 g N/plant (0.6 g N pre-vegetation + 0.3 g N after the first harvest). After the fertilization levels: 0.9 g N and 1.2 g N/plant, the leaves of basil cv. Wala were characterized by the most desired aromatic discriminants: basil-like, sharp, spicy and herb-like.
Safe production of leafy vegetables is more important than other horticultural crops due to their significant contribution in health promotion of consumers. In present study, the growth and nutritional quality of sweet basil plants (Ocimum basilicum L.) were evaluated under some organic compared to chemical NPK fertilizing treatments. A non cultivated soil was used in the experiment, and treatments were arranged in completely randomized design with four replications. Manure in 20 and 40% of pot volume, vermicompost in 20 and 40% of pot volume, biophosphate, NPK fertilizer and a no fertilizing control were applied to plants. The results showed that plant growth and biochemical characteristics were differently affected by organic and chemical fertilization treatments. Vermicompost particularly in 20% of pot volume resulted in highest growth and quality parameters, while the lowest values recorded in 40% manure treatment. The leaf content of ascorbic acid, essential oil, protein and minerals (P, K, Mn, Cu) were highest in 20% vermicompost, whereas leaf content of Mg, Fe and Zn was highest in 40% vermicompost treatment. Leaf nitrate content was significantly reduced in all organic treatments than NPK fertilization. Biophosphate also improved the yield and quality traits compared to unfertilized plants, while manure in both quantities reduced most of the traits (leaf vitamin C, oil yield and protein content) except the content of phenols. This indicates that processing as vermicompost can be a better way of manure application for basil production.
The aromatic and curative properties of basil are connected with the presence of essential oil, mainly in the leaves and flowers. Herb yield and the quality of basil oil are related to the effect of genetic, ontogenetic and environmental factors. In the studies conducted in the years 2008-2009, the effect of basil cultivar and foliar feeding upon selected flowering features was examined. In an unheated plastic tunnel, plants of Kasia, Wala, Genua Star and Opal cultivars were grown. Foliar feeding was done by the application of 0.5% urea solution or by spraying the plants with water. During harvest the characteristic features of flowering were determined, i.e. the number and length of inflorescences, as well as the number of verticils and flowers per inflorescence. A vast differentiation was demonstrated in flowering of sweet basil plants of the examined cultivars. The most inflorescences were formed by the plants of the Polish cultivar Kasia, compared to the remaining ones. The longest inflorescences with the highest number of flowers in the main inflorescence were found in ‘Genua Star’. Foliar feeding of the plants with nitrogen caused a significant increase of inflorescence number, but it did not significantly affect the remaining features of basil flowering.
The experiment was conducted in the Research Institute of Medicinal Plants in Poznań, Poland in 2005–2006 and concerned a collection of ten sweet basil cultivars such as: ‘Minette’, ‘Mittelgrossblättriges Grünes’, Piccolino’, ‘Rubin’, ‘Red Rubin’, ‘Genoveser’, ’Martina’ and two Polish cultivars: ‘Wala’ and ‘Kasia’. Moreover, ‘Lemonette’ cultivar of Ocimum americanum L. was also included in the collection. The content of essential oil in the dried herb was estimated and its composition was analyzed. The content of essential oil in the dried herb without stems ranged from 0.23% (‘Red Rubin’) to 1.67% (‘Kasia’). The main component of the oils that came from nine basil cultivars was linalool, (from 37.07% for ‘Piccolino’ to 76.22% for ‘Minette’). Considerable amounts of 1,8-cineol and eugenol, next β-pinene, α-terpinene and myrcene were also found. The content of 16 identified components in oil represented from 69.34% (‘Piccolino’) to 96.11% (‘Wala’) of the GC profiles. Both Polish cultivars were distinguished by high content of geraniol in essential oil.
Wzrastające nawożenie azotowe miało istotny wpływ na plon ziela bazylii wonnej (Ocimum basilicum L.). Najwyższy plon liści, łodyg oraz ziela uzyskano stosując dawkę 1.8 g N/roślinę (1.2 g przedwegetacyjnie + 0.6 g N/roślinę po I zbiorze). Plony zebrane w II terminie były wyższe od plonów z I terminu.
Sweet basil (Ocimum basilicum L.) is an annual plant belonging to the plant family Lamiaceae. There are many varieties of basil, with leaves of colour from green to purple and with small white or pink flower. In field experiment conducted in the years 2007–2008 there was investigated the effect of nitrogen fertilization in the doses of 50, 150 and 250 kg N·ha-1and irrigation on yielding and nutritional status of basil of ‘Red Rubin’ cultivar grown from transplants. Basil positively responded to irrigation and the increase in its yield under the influence of irrigation amounted from 97.37% to 25.36% in subsequent years of cultivation. Also the dose of nitrogen did advantageously affect basil herb yield. The highest yields were recorded at the dose of 150–250 kg N·ha-1. The highest amount of nitrates ranged from 697.00 to 938.00 mg·kg-1 f.m. was determined in plants fertilized with nitrogen dose of 250 kg N·ha-1. The level of macroelements depended both on irrigation and on nitrogen doses. Irrigation caused decreased amounts of phosphorus, potassium and calcium, while content of magnesium increased. The highest quantities of phosphorus and magnesium were obtained at nitrogen dose of 150 kg N·ha-1, while for the highest values of potassium and calcium nitrogen dose was 250 kg N·ha-1.
The aim of this study was to examine the effects of management practices and biofertilization on microbial activity in rhizosphere and yield of medicinal and aromatic plants. Field experiment was performed using four plant species: peppermint (Mentha × piperita L.), pot marigold (Calendula officinalis L.), sweet basil (Ocimum basilicum L.), and dill (Anethum graveolens L.). Treatments were arranged in a split-plot layout in four replicates using basic plots under conventional and organic management, and subplots with and without biofertilizer (Azotobacter chroococcum). Organic management positively affected the microbial number and activity. Biofertilization increased the total microbial number (13–21%), number of ammonifiers (13–60%), nitrogen-fixing bacteria (7–36%), actinomycetes (36–50%), fungi (60–100%), cellulolytic microorganisms (57–217%), dehydrogenase (28–52%) and ß-glucosidase activity (15–39%). The effects of management practices and biofertilization were highly significant for the yield of examined plants. The yields were higher on inoculated treatments both in conventional (5–26%) and organic (7–15%) growing system.
A field experiment in growing sweet basil was carried out in the period 2008-2010 in Fajsławice (Lublin region), on podzolic soil. The study evaluated the biometric traits of the plants, yield, the qualitative parameters of herbal raw material and weed infestation of the crop in dependence on growth simulators (Asahi SL, Bio-algeen, Titanit) and the forecrop (winter wheat or spring barley + white mustard cover crop). Plots without foliar application of the growth stimulators were the control treatment. Tillage, mineral NPK fertilization as well as mechanical and chemical weed control were typical for this plant species and consistent with the recommendations for herbal plant protection. A hypothesis was made that the application of growth stimulators would have a positive effect on basil productivity, raw material quality and weed infestation of the basil crop. It was also assumed that the phytosanitary and fertilizing effects of the cover crop would result in higher and qualitatively better yield compared to the cereal forecrop alone (winter wheat). The best quantitative parameters of sweet basil raw material and the highest reduction in air-dry weight of weeds in the crop were observed after the application of the growth stimulators. The forecrop – spring barley + a white mustard cover crop that is ploughed in – also had a beneficial effect on yield and weed infestation of the plant in question. The traditional crop protection method used in the basil crop, without the application of the growth stimulators, resulted in a lower plant height and a smaller number of shoots per plant. This caused higher weed infestation of the crop and a decrease in yield. The positive side of the non-application of growth stimulators was a better chemical composition of basil raw material. Asahi SL and Tytanit yielded the best growth and productivity of the basil plants.
Within the Ocimum genus there occur about 200 species in different varieties and forms. They differ in the essential oil content and quality, as well as in many morphological features. The studies conducted in the years 2008–2009 were aimed at defining the effect of basil cultivar (‘Kasia’ and ‘Wala’), as well as of the differentiated doses of CaCO3 (6 or 12 g·dm-3 of substratum) upon the biological value and chemical composition of the oil in the examined plants, grown in pots filled with transitory peat. A significant interaction was reported between the examined cultivar and CaCO3 dose upon the yield of fresh basil plant weight. The highest yield (172.3 g·plant-1) was obtained from plants of ‛Kasia’ cultivar, when a higher dose of CaCO3 was applied (12 g·dm-3 substratum). Lack of significant effect of calcium carbonate dose was reported upon the yield of fresh basil plant weight. In the examined objects similar fresh weight yield values were obtained. At a higher dose of calcium carbonate the unit plant weight equaled 165.9 g·plant-1, and at a lower dose of CaCO3 it was 160.2 g·plant-1. The percentage of dry matter significantly depended on the cultivar. Basil of ‘Wala’ cultivar had higher dry matter content, (32.0%) compared to ‘Kasia’ (25.6%). Both the cultivar and calcium carbonate dose significantly affected the vitamin C contents in fresh basil herb. Increasing the CaCO3 dose from 6 g·dm-3 to 12 g·dm-3 caused the increase of vitamin C concentration by 12.6%. Higher essential oil contents was characteristic of the herb of ‘Kasia’ plants – 1.33% compared to ‘Wala’ cultivar, in whose herb 1.03% of oil was determined. The qualitative composition of isolated oil depended on the examined factors. The predominating compounds were: linalool, 1.8-cineole, geranyl, D germacrene, γ-cadinene, Epi-α -cadinole. A certain differentiation was found in the contents of particular compounds in the essential oils of ‘Kasia’ and ‘Wala’ plants, as the effect of calcium nutrition.
Basil, cultivated as a medicinal and seasoning plant, has strict requirements as to climate, as well as soil fecundity and humidity. The herb harvested during flowering period, contains, besides valuable essential oil, plant dye, vitamins and mineral components. The undertaken studies were aimed at analyzing the contents of macro and microelements in the herbs of two basil cultivars (‘Kasia’ and ‘Wala’), depending on the doses of calcium carbonate – 6 and 12 g·dm-3 substratum. The total nitrogen contents was on quite an even level – from 5.23 to 5.43% d.m. A significant effect of the examined cultivar was reported upon the N-total contents, whereas the calcium carbonate dose did not differentiate the content of this component in a plant. Significant effects of calcium fertilizer dose and cultivar were reported upon potassium and calcium contents in basil herb. The double dose of CaCO3 caused the decrease of potassium and calcium amounts in the plant. Higher concentrations of K and Ca were found in the herb of ‘Kasia’ than ‘Wala’ cultivar. Higher zinc, manganese and copper contents were found in plants of ‘Wala’ cultivar, as compared to plants of ‘Kasia’ cultivar. Doubling the dose of calcium carbonate caused the decrease of manganese, copper and iron concentrations in basil herb, as compared to a single dose. Only as to zinc no such response was reported. The concentrations of nutrients in the examined substratum was differentiated depending on the analyzed factors.
In 2005-2007, in a field experiment, the yield and quality of the basil herb of the Polish cultivar Kasia in organic farming were tested. The experiment was established on six organic farms and one conventional farm in different location in Poland. The following features were evaluated: fresh and dried herb yield, stem fraction in herb, seed yield, weight of 1000 seeds, essential oil content, macro- and microelements content and microbiological purity. Only from one organic farm in Słońsk basil herb was yield higher compared with the yield from conventional cultivation, although it contained a high amount of stems. Organic basil herb was characterized by a high content of essential oil and an increased content of macro- and microelements. Evaluation of microbiological purity showed that herb contamination from both types of cultivation did not exceed the standard for raw materials treated with hot water.
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