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The aim of this study was to analyze data collected by the SENTINEL influenza surveillance system in Poland in the first post-pandemic season 2010/2011. The results include weeks 35/2010–17/2011. Physicians registered weekly number of influenza-like illnesses and collected specimens. Laboratory tests were done using PCR and/or real-time PCR or immunofluorescence. Laboratories also isolated the influenza virus. Influenza-like illness incidence amounted to 2802.7/100000. Weekly incidence ranged from 11.3/100000 to 232/100000. The most affected group was children 0–4 years. Influenza-like illness peak occurred between weeks 02/2011 and 11/2011. Influenza infections were confirmed in 34.9% of specimens. More cases were caused by influenza A, including A(H1N1)pdm09 than influenza B (59.9% vs. 40.1%). The isolated strains were similar to A/California/7/2009 or B/Brisbane/60/2008. Season 2010/2011 in Poland did not differ from the rest of Europe. Further improvement is necessary, especially in the area of specimen collection at the beginning of an epidemic season and carrying out the isolation of the influenza virus.
Birth defects (BD) surveillance using international standards was introduced in Ukraine by a network of five BD centers located in northwestern, central and southern regions. BD centers provide resources to access current and comprehensive information and to nurture partnerships with physicians, administrators, parental support groups, educators, and humanitarian assistance organizations. One outcome was the vigorous and popular website International BD Information Systems (IBIS). The network is now incorporated as OMNI-Net Ukraine. The program has documented high prevalence rates of neural tube defects (NTD); fetal alcohol effects (FAE); and idiopathic developmental retardation among orphans that prompted prevention and amelioration initiatives. Further program objectives include: universal folic acid flour fortification, as recommended by the Ukrainian Academy of Medicine; continued research on methods to reduce FAE in collaboration with partners from California; opening other early infant stimulation centers funded by local authorities, modeled on those in Rivne and Lutsk; and linking BD prevention with bioethical considerations, which is a topic of interest in Ukraine in part enhanced by the effects of Chornobyl.
Patients with malignancy typically exhibit abnormal dendritic cell profiles. Interstitial tumor pressure is increased 20-50mmHg over that in normal tissue. We hypothesized that elevated pressure in the tumor microenvironment may influence dendritic cell (DC) phenotype and function. Monocyte-derived immature and mature DC isolated from healthy human donors were exposed to either ambient or 40 mmHg increased pressure at 37°C for 12 hours, then assessed for expression of CD80, CD86, CD83, CD40, MHC-I and MHC-II. IL-12 production and phagocytosis of CFSE-labeled tumor lysate were assessed in parallel. Elevated pressure significantly increased expression of all co-stimulatory and MHC molecules on mature DC. Immature DC significantly increased expression of CD80, CD86, CD83 and MHC-II, but not MHC-I and CD40, versus ambient pressure controls. Pressure-treated immature DC phenotypically resembled mature DC controls, but produced low IL-12. Phenotypic maturation correlated with decreased phagocytic capacity. These results suggest increased extracellular pressure may cause aberrant DC maturation and impair tumor immunosurveillance.
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