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Edible mushrooms (Agaricus bisporus) were enriched with an irrigation solution containing or not fortified with selenium in form of sodium selenite. Se in control mushrooms was 1.04 mcg/g D.M. and in mushrooms subjected irrigations with water solution of Se this increased to 15 mg of Se/dm) after the first flush and rose to with subsequent flushes to 30.14 mcg/g D.M. Peroxidase activity offresh mushrooms was reduced in Se-enriched Agaricus bisporus. Postharvest storage Peroxidase activity increase for 6 days and then a depression in activity .occutred. A positive correlation between peroxidase activity and fruit body mass of mushrooms was observed. Following fractionation on Sephadex G-100 column 4 peaks of peroxidases activity was observed in water extracts of mushrooms regardless of Se treatment. Molecular mass of these protein fractions were estimated by molecular mass markers as 82, 57, 45 and 22 kDa. Substantial part of peroxidase activity appears to originated from Se-dependent glutathione peroxidase.
The aim of the study was to determine the effect of linseed and rapeseed dietary supplementation on the fatty acids profiles of two ostrich fat depots: breast and subcutaneous (above the leg). The study was carried out on 40 ostriches raised in five groups – control (C) or with 4% (L4) or 8% (L8) linseed, or 5% (R5) or 10% (R10) rapeseed in the diet, from hatching to 12 months of age. Fat samples of breast (BF) and leg fat (LF) were taken for fatty acids analysis. Generally ostrich fat has high contents of PUFA (BF – 23.9, LF – 20.2 g/100 g FAME), especially linoleic acid (BF – 16.4, LF – 12.5 g/100 g FAME) and linolenic acid (BF – 5.7 and LF – 6.2 g/100 g FAME). Ostrich BF had a higher content of n-6 FA and total PUFA and lower n-3 FA than the LF. Both fat depots had desired PUFA/SFA ratios above 0.4, but not desirable n-6/n-3 ratios. BF had significantly higher (0.69)PUFA/SFA ratio than LF (0.55). Both L4 and L8 caused higher total PUFA content (27.8, 25.6 g/100 g FAME, respectively) and higher PUFA/SFA ratios (0.74, 0.75, respectively) and lower n-6/n-3 ratios (1.5, 1.8, respectively) compared to C. The rapeseed supplementation decreased the LA content in ostrich fats (R5- 14.1, R10-13.4g/100g FAME), causing a lower n-6/n-3 (4.1, 4.6, respectively) ratio compared to C (6.1). The supplementation of ostrich diets with linseed improved the nutritional value of ostrich fat by increasing the n-3 FA, total PUFA content and PUFA/SFA ratio. Although the leg fat had a lower PUFA content, both depots of ostrich fats can be recommended as valuable ingredients for value-added meat products fit for human consumption.
The effect of nettle extract supplement for fattening pig feed on meat quality was assessed on 42 pigs initially weighing about 60 kg and about 110 kg at the end of the experiment. All pigs were fed with a standard finisher feed mixture with no supplement in group I (control) and supplemented with 500 mg or 1000 mg of nettle extract per 1 kg of feed in groups II and III, respectively. Commercial water extract from common nettle containing 5.6 mg of tannins per 1 kg was used as a supplement. The meat of pigs receiving a higher dose of extract contained significantly more protein and less fat than those from both remaining groups. A supplement of nettle extract increased the lightness of meat and stabilized meat color for 6 months of storage at 20°C. Moreover, it slightly improved meat oxidative stability during frozen storage and raised polyunsaturated fatty acids (PUFA) content mainly due to diminishing monounsaturated fatty acids (MUFA) content. It was claimed that water extract from nettle had a positive effect on meat quality improving oxidative stability and the polyunsaturated / saturated fatty acids ratio.
Vicia faba plants were grown under drought conditions and variously supplemented with calcium. Drought stress markedly inhibited the growth of Vicia faba plants. Ca²⁺ ameliorated to a large extent this inhibition; fresh weight, dry mass, chlorophyll and water contents were variably improved. Membranes were, also, negatively affected by drought stress and percentage leakage was elevated. Concomitantly, the efflux of K⁺ and Ca²⁺ was enhanced by drought but lowered by supplemental Ca²⁺. In addition, membranes of droughted plants were sensitive to the Ca²⁺ channel blockers lanthanum, nifedipine or verapamil more than those of control plants. These blockers significantly increased the efflux of K⁺ and Ca²⁺ as well as percentage leakage particularly in those of droughted plants. The above results indicated that the functioning of the calcium channels was negatively affected when Vicia faba was grown under drought conditions. However, much of the drought-induced disorders including sensitivity towards the applied calcium channel blockers could be ameliorated by supplemental Ca²⁺.
A total of 150 one-day-old male broiler chicks (Ross 308) and 120 one-day-old female Muscovy ducklings were distributed over 15 and 12 pens, respectively. All birds received the same diet during the first period of life. Throughout the second period (36-56 days for broiler chickens and 43-69 days for Muscovy ducks) different source plant extracts were supplemented to the basal diet for each species; dietary treatments were assigned to three pens each. In the chicken (CK) trial the following dry extracts were tested: tomato (Solanum lycopersicum) skin (200 mg lycopene kg-1 feed; CK-L200 group), orange (Citrus aurantium) peel (200 mg hesperidin kg-1 feed; CK-O200 group), and green tea (Camellia sinensis) leaves (200 mg catechins kg-1 feed; CK-T200 group). For the Muscovy duck (DK) trial the tested extracts were produced from rosemary (Rosmarinus officinalis) leaves (200 mg carnosic acid kg-1 feed; DK-R200 group) and orange (Citrus aurantium) peel (200 mg hesperidin kg-1 feed; DK-O200 group). The effects in both species were compared with those for the unsupplemented diet (CK-C and DK-C) and the diet supplemented with 200 mg of alpha-tocopheryl acetate (CK-E200 and DK-E200). At the end of each trial three birds per pen were slaughtered. Growth performance,pH and meat proximate composition in both species were not affected by dietary treatments. The TBARS value of chicken leg meat from the unsupplemented group was 3.86, while on average in CK-E200, CK-L200 and CK-O200 it was by 60, 55 and 63% lower (P<0.05), whereas in CK-T200 it was by 25% higher (P<0.05). Dietary treatments did not exert any antioxidant effects on chicken breast meat. The TBARS value of duck breast meat and leg meat from the control was 1.39 and 4.51,respectively, while on average in the DK-E200, DK-O200 and DK-R200 groups it was by 82 and 71%, 33 and 46%, and 66 and 47% lower (P<0.05), respectively. The magnitude of the antioxidant action of vegetable dry extract in this trial was lower than that of alpha-tocopheryl acetate.
The aim of this study was to investigate the effect of platelet activating factor (PAF) on the quality characteristics of cryopreserved canine spermatozoa. Cryopreserved semen of 5 mixed-breed dogs was treated with different concentrations of exogenous PAF (1 × 10⁻³M, 1 × 10⁻⁴M, 1 × 10⁻⁵M and 1 × 10-6M) and examined at different time intervals (0, 30, 60 and 120 min). Cryopreserved semen treated without PAF was used as the control. Sperm quality was evaluated for motility (computer-assisted semen analysis, CASA), mitochondrial function (JC-1/PI assay) and plasma membrane integrity (SYBR-14/PI assay and Hoechst 33258). Also, ATP content of spermatozoa was determined using a bioluminescence assay. Treatment of cryopreserved semen with 1 × 10⁻³ M PAF at 120 min of incubation resulted in significantly higher total sperm motility compared with the control. It was observed that PAF-improved total sperm motility was concurrent with enhanced sperm motility patterns after treatment of cryopreserved semen. Treatment of cryopreserved semen with PAF did not improve either sperm mitochondrial function or plasma membrane integrity, as monitored by different fluorescent membrane markers. Furthermore, ATP content of cryopreserved spermatozoa was significantly higher when PAF was used at a concentration of 1 × 10⁻³ M compared with the control and other PAF treatments, regardless of the incubation time. The findings of this study indicated that treatment with 1 × 10⁻³ M PAF at 120 min of incubation rendered better quality of cryopreserved canine semen, which was associated with improved sperm motility parameters and ATP content. It can be suggested that exogenous PAF addition is beneficial as a supplement for canine semen extender used for cryopreservation.
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