Wyniki wyszukiwania

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Molecular characteristics of sucrose synthase isolated from bird cherry leaves

100%

Sytykiewicz H., Czerniewicz P., Leszczynski B.

Herba Polonica

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2008

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tom 54

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nr 3

The molecular characteristics of partly purified sucrose synthase (NDP-glucose: D-fructose 2-α-D-glucosyltransferase, EC 2.4.1.13), extracted from the bird cherry (Prunus padus L.) leaves was elucidated. The sucrose synthase was successfully purified by using a four-step protocol including ammonium sulfate precipitation, dialysis, gel filtration on Sephadex G-150 and ion-exchange chromatography with the use of DEAE-cellulose. The analysed enzyme occurred in two isoforms (SuSyI and SuSyII). The relative molecular weight of native isoenzymes was estimated to be 200 and 180 kDa, respectively. Isoform SuSyI contained two different subunits of 57.5 and 52.8 kDa, whereas the structure of SuSyII was consisted of identical 63 kDa subunits. Experimental data indicated that the structure of both SuSy isoforms was composed of three subunits

2

Relationship between sucrose accumulation and activities of sucrose-phosphatase, sucrose synthase, nautral invertase and soluble acid invertase in micropropagated sugarcane plants

100%

Gutierrez-Miceli F. A., Rodriguez-Mendiola M. A., Ochoa-Alejo N., Mendez-Salas R., Dendooven L., Arias-Castro C.

Acta Physiologiae Plantarum

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2002

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tom 24

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nr 4

3

Variation in gene transcription and protein for key enzymes of carbohydrate metabolism in poplar xylem with respect to growth phase

84%

Alluvada J., Fokar M., Holaday A. S.

Acta Physiologiae Plantarum

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2014

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tom 36

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nr 09

We compared gene expression levels for enzymes of carbohydrate metabolism in the twig xylem of two Populus species with the seasonal levels of starch and soluble sugars (sucrose, glucose, and fructose) and relative levels of the enzymes. Plants of Populus deltoides Bartr. ex Marsh and P. balsamifera L., 3–4 years old, were grown outside in Lubbock, TX, USA in 43 L pots. The xylem in the middle portion of the twigs was sampled during the dormant period (November–February), at bud break (for P. balsamifera), and during the growth flush (April–July). The gene expression for ADP-glucose pyrophosphorylase (AGPase), sucrose synthase (SuSy), and sucrose-phosphate synthase (SPS) generally coincided with the levels of the carbohydrates in whose metabolism these enzymes are involved. Gene expression for AGPase and its protein levels were high when the xylem starch content was high (growing period). However, P. balsamifera maintained high AGPase levels in dormant and growing twigs, unlike P. deltoides whose dormant twigs had low AGPase and low gene expression. Compared to growing twigs, gene expression for SuSy and SPS and their protein levels were higher in dormant twigs when soluble sugar content was higher. No down-regulation of these genes appears to occur when pools of the associated carbohydrates are high. Contrary to our expectation, the gene expression for bamylase was highest in growing twigs when starch content was high. High β-amylase gene expression in growing twigs may be involved in maintaining a sufficient level of soluble sugars for growth through possibly controlling the extent of starch accumulation.

4

Regulation of sucrose storage by amino acid arginine in sugarcane cell suspension cultures

84%

Sachdeva M., Mann A. P. S., Batta S. K.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 1

Sugarcane cell cultures were obtained from callus formed on explants derived from young expanding leaves of two early maturing sugarcane varieties viz “CoJ83” and “CoJ86”. The cell cultures were varied with different arginine concentrations in the culture medium. For each cultivar, sucrose content with 20 μM arginine in the culture medium decreased from 3 to 5 days and then increased to 10 days after subculturing. Higher concentration of arginine in the culture medium (60 μM) decreased the sucrose content at different days after subculturing and thus significantly stimulated sucrose mobilization. The activity of sucrose synthase and sucrose phosphate synthase reached maximum while the activity of acid and neutral invertase was minimal in the culture medium with 20 μM arginine. Thus arginine at low concentration (20 μM) enables the cells to accumulate the higher level of sucrose. The optimum level of amino acids can be utilized to regulate the in vivo activity of sucrose synthase, sucrose phosphate synthase and invertase to achieve maximum sucrose accumulation in sugarcane storage tissue.

5

The effect of sulphite on chlorophyll fluorescence and sucrose metabolism in poplar leaves

84%

Lorenc-Plucinska G., Szadel A., Plucinski A., Matysiak R.

Acta Physiologiae Plantarum

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2002

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tom 24

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nr 2

6

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Sucrose uptake and transport are inhibited by okadaic acid during regeneration of the sugar-starved Vicia faba root meristem cells

67%

Polit J. T., Zabka A.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 3

7

Sink development, sucrose metabolising enzymes and carbohydrate status in turnip [Brassica rapa L.]

67%

Gupta A. K., Singh J., Kaur N.

Acta Physiologiae Plantarum

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2001

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tom 23

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nr 1

8

Sucrose degradation in sink and source poplar leaves treated with sulfite

67%

Lorenc-Plucinska G., Szadel A., Plucinski A., Pukacka S.

Acta Societatis Botanicorum Poloniae

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2001

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tom 70

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nr 3

9

Activities of sucrolytic enzymes in developing pods of lentil genotypes differing in seed size

67%

Chopra J., Kaur N., Gupta A. K.

Acta Physiologiae Plantarum

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2005

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tom 27

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nr 1

The activities of sucrolytic enzymes viz. sucrose synthase and invertases were compared in developing pods of two genotypes of lentil differing in seed weight. Biomass accumulation of both the podwall and seed of ‘large’ genotype was higher during development as compared to the ‘small’ genotype. High activity of acid invertase together with prolonged activity of alkaline invertase in podwall of ‘large’ genotype may lead to longer cell division phase resulting in its larger size and biomass. Greater biomass of podwall could be responsible for providing more reserves for the developing seed hence determining its size. Higher alkaline invertase activity in ‘large’ seed from 15-20 DAF can be correlated to the sustained sucrolytic conditions for producing more cells required for its larger size. In creased levels of sucrose synthase in ‘large’ seed especially during maturation phase suggest the role of this enzyme in enhancing the seed sink strength.

10

The influence of chilling on photosynthesis and activities of some enzymes of sucrose metabolism in Lycopersicon esculentum Mill.

67%

Artuso A., Guidi L., Soldatini G. F., Pardossi A., Tognoni F.

Acta Physiologiae Plantarum

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2000

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tom 22

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nr 2

Ograniczanie wyników

Molecular characteristics of sucrose synthase isolated from bird cherry leaves

Relationship between sucrose accumulation and activities of sucrose-phosphatase, sucrose synthase, nautral invertase and soluble acid invertase in micropropagated sugarcane plants

Variation in gene transcription and protein for key enzymes of carbohydrate metabolism in poplar xylem with respect to growth phase

Regulation of sucrose storage by amino acid arginine in sugarcane cell suspension cultures

The effect of sulphite on chlorophyll fluorescence and sucrose metabolism in poplar leaves

Sucrose uptake and transport are inhibited by okadaic acid during regeneration of the sugar-starved Vicia faba root meristem cells

Sink development, sucrose metabolising enzymes and carbohydrate status in turnip [Brassica rapa L.]

Sucrose degradation in sink and source poplar leaves treated with sulfite

Activities of sucrolytic enzymes in developing pods of lentil genotypes differing in seed size

The influence of chilling on photosynthesis and activities of some enzymes of sucrose metabolism in Lycopersicon esculentum Mill.