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The aim of the work was to determine the influence of initial sucrose concentration in fermentation medium on oxalic acid synthesis by Aspergillus niger W78C in submerged batch culture. Cultivations were conducted in synthetic medium containing sucrose in the concentration of 100, 125, 150, 175 or 200 g·dm⁻³. As the result of the present investigation, the optimal sucrose concentration was found at the level of 125 g·dm⁻³. In medium containing this amount of carbon source, 70.2 g·dm⁻³ of oxalic acid was obtained. When substrate concentration was the highest, 200 g dm⁻³, the amount of product was only 5.2 g·dm⁻³ higher than in medium with 125 g·dm⁻³ of sucrose. Moreover, an increase of the initial sucrose concentration resulted in process time extension, an increase of citric and gluconic acids concentration and a decrease of productivity and oxalic acid yield.
Six seedling hypocotyl anatomical characters of sugar beet diploid lines and triploid hybrids were measured. Root yield and sugar content of these lines and hybrids were evaluated in replicated field trials. Some of the studied hypocotyl characters: the diameter of the central core, the diameter of parenchymatic cells outside the central core and the width of xylem, correlated negatively with sugar content and positively with root yield. This suggests that these parameters can be used in preliminary selection of sugar-beet breeding material. Introducing such criteria into the breeding process could speed up the selection and reduce the number of expensive field trials.
The aim of this study was to evaluate the influence of sucrose concentration in the preculture medium, as well as the duration of osmotic dehydration on the efficiency of chrysanthemum ‘Richmond’, ‘Lady Orange’ and ‘Lady Salmon’ cryopreservation by encapsulation-dehydration technique. For all cultivars tested, the best regrowth of cryopreserved shot tips was recorded with 0.25 M sucrose concentration and 10 µM ABA during a two-week preculture, followed by a 4- or 7-day osmotic dehydration. The survival rate ranged from 56.8–58.0% (the Lady group) to 63.6% (‘Richmond’). However, the ability to grow was smaller and reached 18.2–50.7%. It was found that higher sucrose concentration during the preculture slowed the growth of chrysanthemum shoot tips and led to an increased formation of multiple shoots (by activating axillary buds) or deformed adventitious shoots (incapable of further growth). The frequency of tissue hyperhydricity also increased, while the rhizogenesis efficiency decreased when higher sucrose concentration in the preculture medium was applied. The influence of osmotic dehydration duration on the explants morphogenetic response was cultivar-dependent.
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