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Suckling mice and immunosuppressed adult mice were used as model hosts to compare the endogenous development of two isolates of C. parvum obtained from naturally infected calves in different seasons of the year. There were no differences in these two isolates as to their location, time of appearance, intensity of infection and antigenicity. Present study indicates that Cryptosporidium infection may be easily established in 5-7 day old outbred Swiss mice. The peak of infection is shown on day 8 after inoculation and shortly after that a self-limiting process of infection takes place. However, C. parvum does not develop endogenously in adult immunosuppressed mice. The lack of establishment of infection in these animals, even in latent state, indicates that age dependent mechanisms of immunity, developed in mice, are not impaired by prednisolone or cyclophosphamide.There was no difference in biological characteristics of two isolates C. parvum. Moreover, the present study illustrates that oocysts, spread by naturally infected calves in autumn and winter, are infective for suckling mice, which might be associated in transmissibility of C. parvum.
Comparison of the results of rearing the suckling piglets, receiving different Fe preparations. The aim of the studies was determine the results of rearing the piglets which received three different preparations of iron (Fe) by injection. The sows, the progeny of which was covered with the observations, were at random assigned before parturition, to one from three experimental groups – E1, E2 and E3 (5 sows in each group). The rate of growth, feed utilization and health state of the piglets from birth until weaning (35 day of life) was controlled. The highest body weight of 5-week old piglets was recorded in group E3; the difference in relation to body weight of the piglets from group E1 and 2 was equal to 3.47% and 13.15%, respectively (p>0.05). The best equalization of body weight of 5-week old piglets was found in groups E3 and E1. The piglets from group E3 consumed the greatest quantity of fixed feed until weaning; in the mentioned group, the body weight gain in total was the highest one (8.44 kg/head). The mean daily gains of the piglets from group E3 were equal to 241 g and they were higher as compared to group E1 and E2 by 5.24% and 12.62%, respectively. Consumption of solid feed per gain of 1kg of body weight of the litter amounted in groups E1, E2 and E3 to 4.80, 3.49 and 4.65 kg, respectively. The mentioned index was low what indicates a good milk performance of the sows. All three employed preparations fulfilled their prophylactic function and prevented incidence of anaemia symptoms.
Nowak J.: Insulin and glucose concentration changes in newborn piglets after sucking the colostrum from insulin administered sows. Acta Physiol. Pol. Immuno reactive insulin (IRI) concentration in sows colostrum has been previously proven to be much higher than that in blood. The experiment was carried out to show the influence of endogenous and added insulin in sows colostrum on insulinaemia and glycaemia of newborn piglets. In colostrum collected from 3 control and 5 experimental sows before loading, basal insulin concentration were 1.595 and 1.365 nM⁻¹ , respectively, and calculated for all 8 sows together were 1.451 nM⁻¹ (SEM ± 0.289). Basal plasma insulin concentrations calculated for 68 healthy piglets before sucking were little differentiated (mean 0.318 ± 0.044 nM I⁻¹ ), whereas glucose initial concentrations for those piglets (mean 3.581 ± 0.275 nM I⁻¹ ) were highly differentiated. Intramuscular loading of 5 experimental sows with insulin (80 I.U. per animal) caused an increase in the concentration of insulin in colostrum from 1.365 to 3.449 nM I⁻¹ (0.01, P < 0.02). The mean insulin level (0.313 + 0.04 nM I⁻¹ ) in experimental piglets blood plasma (n = 42) increased significantly to 1.234 + 0.07 (P < 0.001) after suckling by sows loaded with exogenous insulin. Glycaemic response of those two piglets litters was poor but showed a statistically significant increase (P < 0.001). The glucose concentrations in blood plasma samples of the other three litters did not changes after sucking. The experiment excluded the hypothesis that high level of insulin in colostrum could be the cause of hypoglycaemia in healthy piglets after sucking.
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