Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: subunit composition

Sortuj według:

Ogranicz wyniki do:

Molecular characteristics of sucrose synthase isolated from bird cherry leaves

100%

Sytykiewicz H., Czerniewicz P., Leszczynski B.

Herba Polonica

2008

tom 54

nr 3

The molecular characteristics of partly purified sucrose synthase (NDP-glucose: D-fructose 2-α-D-glucosyltransferase, EC 2.4.1.13), extracted from the bird cherry (Prunus padus L.) leaves was elucidated. The sucrose synthase was successfully purified by using a four-step protocol including ammonium sulfate precipitation, dialysis, gel filtration on Sephadex G-150 and ion-exchange chromatography with the use of DEAE-cellulose. The analysed enzyme occurred in two isoforms (SuSyI and SuSyII). The relative molecular weight of native isoenzymes was estimated to be 200 and 180 kDa, respectively. Isoform SuSyI contained two different subunits of 57.5 and 52.8 kDa, whereas the structure of SuSyII was consisted of identical 63 kDa subunits. Experimental data indicated that the structure of both SuSy isoforms was composed of three subunits

Role of the non-catalytic subunits of the eukaryotic respiratory complexes. Why are these complexes much larger than their prokaryotic equivalents?

100%

Boumans H., Berden J. A., Grivell L. A.

Cellular and Molecular Biology Letters

1998

tom 03

nr 3

The eukaryotic respiratory complexes carry out conserved reactions compared to their prokaryotic equivalents and contain conserved catalytic subunits. Despite these resemblances, eukaryotic complexes are more complex, containing additional subunits. Various lines of evidence suggest that these additional subunits protect against proteolysis, modulate catalytic activity and/or play a role in assembly. We suggest, however, that none of the present-day functions was the original driving force for their acquisition by the complex during evolution of the mitochondrial respiratory chain and that the incorporation was largely accidental, the small accessory subunits possibly being derived from imported presequences.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Herba Polonica

1 Berden J. A.

1 Boumans H.

1 Czerniewicz P.

1 Grivell L. A.

1 Leszczynski B.

1 Sytykiewicz H.

1 2008

1 1998

Wyniki wyszukiwania

Molecular characteristics of sucrose synthase isolated from bird cherry leaves

Role of the non-catalytic subunits of the eukaryotic respiratory complexes. Why are these complexes much larger than their prokaryotic equivalents?