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Improvement of tannase production under submerged fermentation by Aspergillus niger FBT1 isolated from a mangrove forest

100%

George D. S., Ong C.-B.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2013

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tom 94

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nr 4

Aspergillus niger FBT1, a local extracellular strain for tannase production, was isolated from soil collected from Matang Mangrove Forest Reserve in Perak, Malaysia. This fungus strain was cultivated in an Erlenmeyer flask under a submerged fermentation system. Medium compositions play a very important role in enhancing enzyme production during fermentation. The production of tannase by A. niger FBT1 increased significantly (95%) when the medium compositions and various process parameters were optimized. Incubation for 72 hours (30EC, pH 7) in medium complemented with sodium nitrate was found optimal. Additional supplementation with tannic acid (2% w/v) as the sole carbon source strongly increased the yield of the enzyme.

2

Statistical optimization of alpha-amylase production by probiotic Lactobacillus plantarum MTCC 1407 in submerged fermentation

86%

Panda S. H., Swain M. R., Kar S., Ray R. C., Montet D.

Polish Journal of Microbiology

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2008

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tom 57

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nr 2

Production and purification of α-amylase by probiotic Lactobacillus plantarum MTCC 1407 has been investigated under submerged fennentation using Mann Rogassa Sharpe medium containing (1%) soluble starch in lieu of glucose (2%) as carbon source. Response Surface Methodology was used to evaluate the effect of main variables, i.e. incubation period, pH and temperature on enzyme production. A full factorial Central Composite Design was applied to study these main factors that affected α-amylase production. The experimental results showed that the optimum incubation period, pH and temperature were 36 h, 7.0 and 35°C, respectively. The purified enzyme (by ammonium sulphate precipitation) had a molecular mass of 75 450 Da in SDS-PAGE.

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Study on regulation of growth and biosynthesis of cellulolytic enzymes from newly isolated Aspergillus fumigatus ABK9

72%

Das A., Paul T., Halder S. K., Maity C., Das Mohapatra P. K., Pati B. R., Mondal K. C.

Polish Journal of Microbiology

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2013

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tom 62

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nr 1

This study was aimed to evaluate the pattern of cellulase biosynthesis from Aspergillus fumigatus ABK9 under submerged fermentation. Production was increased concomitantly with fungal growth up to 72 h and reached maximum (Xmax –6.72 g/l) with specific growth rate (µmax) of 0.126/h. Highest specific rate of enzyme production (qp) was found at initial medium pH of 5.0 and incubation temperature of 30°C. At the same time, in the presence of 2-deoxy-D-glucose concentration of 0.5 mg/ml, the production of cellulolytic enzymes, viz, carboxymethyl cellulase activity (CMCase), filter paper degrading activity (FPase) and β-glucosidase activity reached maximum of 132.2, 21.3 and 28.9 U/ml, respectively. Cellulase biosynthesis was induced in respect to higher volumetric production rate (Qp), specific rate of enzymes production (qp, U/g biomass/h) and enzyme/biomass yield (YE/X) when grown in carboxymethyl cellulose in comparison to other saccharides as sole carbon source. Induction ratios (IR) of cellulases were between 12.3 and 24.4 in the presence of 1.5% (w/v) CMC in the culture media. The strain was quite resistant to catabolic repression by glucose up to 0.4% (w/v). Cellulases production was greatly influenced in the presence of yeast extract and potassium dihydrogen phosphate (KH₂PO₄) as nitrogen and phosphate sources in the culture media. C/N ratio of 10.0 and C/P ratio of 4.0 proved to be the best for the production of enzyme cocktail. Along with the high production yield, the crude enzymes showed a promising cellulose hydrolyzing efficiency of rice straw, indicating the enzyme could be beneficial for its large scale industrial exploitation.

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Analysis and glycosyl composition of the exopolysaccharide isolated from submerged fermentation of Ganoderma lucidum CG144

72%

Rubel R., Freire dos Santos L., Stutz Dalla Santa H., Porto de Souza Vandenberghe L., Wojciechowski A. L., de Carvalho J. C., Habu S., Destefanis Vitola F. M., Augur C., Soccol C. R.

Acta Societatis Botanicorum Poloniae

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2014

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tom 83

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nr 3

The evaluation of glycosyl composition is an essential step to guide future research designs applied in bioactivity. In the same way, the unexplored potential bioactivity of exopolysaccharide from Ganoderma lucidum is huge. Therefore, this study investigated the glycosyl composition of the exopolysaccharide isolated from submerged fermentation of G. lucidum to serve as guide for future studies on bioactivity. Glycosyl content and composition were evaluated by combined GC/ MS of the TMS derivatives of the monosaccharide methyl glycosides produced from the sample by acidic methanolysis. Glycosyl composition analysis showed that the dominant carbohydrate component in all samples of exopolysaccharide isolated from submerged fermentation of G. lucidum CG 144 was glucose (58.1%), mannose (26.6%) and galactose (12.5%) which can be referred to as heteroglycan. These results suggest that this Ganoderma exopolysaccharide may be a new immunomodulatory agent.

5

Factors affecting acid protease production by Mucor circinelloides MG603064.1 through SmF process: characterization and fromage frais making

72%

Bensmail S., Boudjema K., Naimi-Fazouane F., Bensmail S., Djouahra-Fahem D., Ferhoum F., Bourfis N.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2023

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tom 104

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nr 4

6

Enhanced production of oxalic acid in Aspergillus niger by the addition of methanol

58%

Rymowicz W., Lenart D.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2004

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tom 07

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nr 2

7

Production of tannase through submerged fermentation of tannin-containing plant extracts by Bacillus licheniformis KBR6

58%

Das M. P. K., Mondal K. C., Pati B. R.

Polish Journal of Microbiology

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2006

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tom 55

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nr 4

Tannins are water-soluble polyphenolic compounds found in plants as secondary metabolites. The presence of these substances in the barks of eight different plants was initially examined and their crude extracts were used separately as a substrate for production of tannase through submerged fermentation by Bacillus licheniformis KBR6. Tannase production as well as biodégradation of the substrate reached a maximum within 15 to 18 h against crude tannin extract obtained from Anacardium occidentale. Among different concentrations of the crude tannin tested, 0.5% (w/v) induced maximum synthesis of enzyme. Tannase production was higher by almost two-fold in the presence of crude tannin compared to pure tannic acid used as a substrate. It seems that industrial production of tannase, using bark extract of A. occidentale can be a very simple and suitable alternative to presently used procedures.

Ograniczanie wyników

Improvement of tannase production under submerged fermentation by Aspergillus niger FBT1 isolated from a mangrove forest

Statistical optimization of alpha-amylase production by probiotic Lactobacillus plantarum MTCC 1407 in submerged fermentation

Study on regulation of growth and biosynthesis of cellulolytic enzymes from newly isolated Aspergillus fumigatus ABK9

Analysis and glycosyl composition of the exopolysaccharide isolated from submerged fermentation of Ganoderma lucidum CG144

Factors affecting acid protease production by Mucor circinelloides MG603064.1 through SmF process: characterization and fromage frais making

Enhanced production of oxalic acid in Aspergillus niger by the addition of methanol

Production of tannase through submerged fermentation of tannin-containing plant extracts by Bacillus licheniformis KBR6