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The aim of the study was to determine the concentration of serum amyloid A (SAA) and the activity of ceruloplasmin (Cp) in milk from cows with clinical mastitis of various severities and with subclinical mastitis in aspect of their usefulness for the detection of mastitis in cows. The concentration of SAA was determined using the commercial ELISA kit. The activity of Cp was determined according to the Rice et al., method. The mean concentration of SAA in milk from cows with mastitis ranged from 4.47 to 322.26 µg/mL. The mean SAA concentration in milk from healthy cows was 11.67 (±7.40) µg/mL and was significantly lower (P<0.01) compared to that in milk from cows with the particular forms of mastitis. The activity of Cp in milk from cows with mastitis ranged from 3.00 to 18.83 U/g of protein. Both in clinical and subclinical mastitis the activity of Cp was significantly higher (P<0.001) compared to that of milk from cows with healthy mammary glands (1.20 ±0.42 U/g of protein). The findings revealed that both the SAA concentration and Cp activity were sensitive indicators of inflammatory processes in the udder, even those graded as mild. Their determination in milk may be a reliable and non-invasive diagnostic method to detect mastitis, particularly its subclinical form.
The investigation was conducted during 2005-2006 on 4010 dairy cows. Having performed statistical data analysis, we determined that the lowest somatic cell count (SCC) in Red and Red-White cow population was obtained when the milking time was 5-6 min., milking speed was higher than 1.5 kg/min., high milk flow was from 2.51 to 4 kg/min., and in Black-White cow population having a milking time was higher than 7 min., milking speed was from 1.01 to 2 kg/min., a high milk flow – from 2.01 to 4 kg/min. (p<0.001). In Red and Red-White cow population with subclinical mastitis, milking time was longer and milking speed was slower than in healthy cows. High milk flow values were least in healthy Black-White cow population. This determines a more equal milk flow which is desired in milking cows mechanically. Most sensitive to udder infection are 1st lactation cows which have a higher milk flow. A larger phenotype correlation coefficient in Red and Red-White cow population was between the SCC and milking time (-0.089, p<0.01) and between high milk flow (0.086, p<0.01) and milk yield (-0.071, p<0.05). However in Black-White cow population, correlation was found between SCC and milk yield (-0.117, p<0.01) and milking speed (-0.110, p<0.01). Contagious mastitis pathogens were identified in Red and Red-White cow milk samples primarily from productive cows having a milking speed of 1.01-1.5 kg/min., and in Black-White cow population having a milking speed of 1.51-2.0 kg/min.
Milk samples were taken from cows with acute, subacute, chronic, and subclinical mastitis and from healthy cows. The mean activity of lactoferrin (LF) in milk from mastitic cows ranged from 8.9 ±3.0 to 12.1 ±6.9 mU/g protein and was significantly lower than that in milk from healthy cows (29.5 ±15.0 mU/g protein). In group of mastitic cows the highest LF activity was found in cows with chronic mastitis, and the lowest in those with subclinical mastitis. The lactoperoxidase activity in cows with clinical and subclinical mastitis was significantly higher in comparison with healthy cows (1.3 ±1.1 mU/g protein) ranging from 5.5 ±2.6 mU/g protein in subclinical mastitic cows to 8.4 ±5.0 mU/g protein in chronic mastitic cows. Lower LF activities in cows with mastitis than in healthy animals may lead to a decreased antioxidant defence system in mastitic cows.
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