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Molecular cloning and characterization of a novel human gene containing 4 ankyrin repeat domains

100%

Li J., Ji C., Zheng H., Fei X., Zheng M., Dai J., Gu S., Xie Y., Mao Y.

Cellular and Molecular Biology Letters

2005

tom 10

nr 1

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Cloning and characterization of a pathogenesis-related gene (ThPR10) from Tamarix hispida

86%

Zhang R., Wang Y., Liu G., Li H.

Acta Biologica Cracoviensia. Series Botanica

2010

tom 52

nr 2

A PR10 gene (ThPR10) was cloned from Tamarix hispida and characterized. Real-time RT-PCR was employed to evaluate gene expression levels. ThPR10 was expressed in both leaves and roots of T. hispida under normal growth conditions, and can be highly induced in both leaf and root tissues by abiotic stresses including NaCl, PEG, cold, CdCl2, and ABA (abscisic acid) treatments. Our results indicated that ThPR10 is involved in the abiotic stress response, and regulated by an ABA-dependent signaling pathway. Subsequently, ThPR10 was localized at the subcellular level. The gene was fused with the GFP N-terminal driven by CaMV35S promoter and transiently expressed in onion epidermal cells. This strategy localized the ThPR10 protein to the nucleus of onion epidermal cells, suggesting that the pathogenesis-related proteins play a functional role in the cell nucleus.

Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori

72%

Pan Y., Xia H., Lu P., Chen K., Yao Q., Chen H., Gao L., He Y., Wang L.

Acta Biochimica Polonica

2009

tom 56

nr 4

671-677

Serpins are a broadly distributed family of protease inhibitors. In this study, the gene encoding Bombyx mori serpin-2 (Bmserpin-2) was cloned and expressed in E. coli. The Bmserpin-2 cDNA contains a 1125 bp open reading frame (ORF). The deduced protein has 374 amino-acid residues, contains a conserved SERPIN domain and shares extensive homology with other invertebrate serpins. RT-PCR analysis showed that Bmserpin-2 was expressed in all developmental stages of B. mori larvae and various larval tissues. Subcellular localization analysis indicated that Bmserpin-2 protein was located in the cytoplasm. Interestingly, real-time quantitative PCR revealed that the expression of Bmserpin-2 in the midgut of susceptible B. mori strain 306 significantly increased at 72 hours post inoculation (hpi) when infected with BmNPV. However, there was no significant increase of the Bmserpin-2 expression in resistant strain NB infected with BmNPV. Thus, our data indicates that Bmserpin-2 may be involved in B. mori antiviral response.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Acta Biologica Cracoviensia. Series Botanica

1 Cellular and Molecular Biology Letters

1 Chen H.

1 Chen K.

1 Dai J.

1 Fei X.

1 Gao L.

1 Gu S.

1 He Y.

1 Ji C.

1 Li H.

1 Li J.

1 Liu G.

1 Lu P.

1 Mao Y.

1 Pan Y.

1 Wang L.

1 Wang Y.

1 Xia H.

1 Xie Y.

1 Yao Q.

1 Zhang R.

1 Zheng H.

1 Zheng M.

1 2010

1 2009

1 2005

Wyniki wyszukiwania

Molecular cloning and characterization of a novel human gene containing 4 ankyrin repeat domains

Cloning and characterization of a pathogenesis-related gene (ThPR10) from Tamarix hispida

Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori