The goal of the research was to evaluate DNA disintegration in boar sperm, which had been diluted at 18ºC in a standard semen diluter to guarantee its usability for insemination purposes for a period of 72 hours from the moment of sampling and diluting. Integrity assessment was carried out twice (i.e. immediately after the sperm had been diluted and brought to 18ºC, and in the 72nd hour) in fall, winter, spring and summer, by means of the APO-BRDU test and a flow cytometer. The degree of defragmentation was follows: autumn – 0 hour: 14.08%, 72nd hour: 33.29%; winter – 0 hour: 22.78%, 72nd hour: 47.15%; spring – 0 hour: 26.13%, 72nd hour: 41.99%; summer – 0 hour: 20.61%, 72nd hour: 33.2%. The results obtained indicate unambiguously that DNA in sperm stored under standard conditions is subject to progressive disintegration. The lowest percentage of disintegration was observed in summer months i.e. in summer and in fall. The highest disintegration was observed in winter.
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