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Salicylic acid (SA), a key signaling molecule in higher plants, has been found to play a role in the response to a diverse range of phytopathogens and is essential for the establishment of both local and systemic-acquired resistance. Recent studies have indicated that SA also plays an important role in abiotic stress-induced signaling, and studies on SA-modulated abiotic tolerance have mainly focused on the antioxidant capacity of plants by altering the activity of anti-oxidative enzymes. However, little information is available about the molecular mechanisms of SA-induced abiotic stress tolerance. Here, we review recent progress toward characterizing the SA-regulated genes and proteins, the SA signaling pathway, the connections and differences between SA-induced tolerances to biotic and abiotic stresses, and the interaction of SA with other plant hormones under conditions of abiotic stress. The future prospects related to molecular tolerance of SA in response to abiotic stresses are also further summarized.
In 2011 year research project on breeding spring barley genotypes tolerant to temporary drought stresses has started. The authors’ responsibility was to test the productivity of genotypes derived from different parental forms obtained in scope of the project for a drought tolerance in pot experiment. In the years 2011–2013 three consequtive series of experiments with approximately seventy genotypes each year have been carried on. Two reference Polish varieties were included into each set. At the control treatment, plants were grown at the optimal soil moisture level of 13–15% weight by weight for the whole vegetation period. Drought stresses were introduced at the tillering stage (BBCH 23) for the period of 11 days or at full fl ag leaf stage (BBCH 45-47) for the period of 14 days. At the both stress treatments, the moisture was maintained at the level of 5–6% weight by weight. Plants were harvested at full maturity stage and the grain and straw yields, and yield components i.e. number of productive tillers, number of grains per spike and weight of 1000 grains were determined. Spring barley showed a higher tolerance to the drought stress at tillering stage than at fl ag leaf stage. Barley genotypes differed in their response to temporary drought stresses due to diverse ability for regenerating after the stress removal. The tolerance of the genotypes to drought stress imposed at tillering stage resulted from their ability to produce additional fertile tillers. The tolerance of the studied spring barley genotypes to temporal drought stress at fl ag leaf stage can be explained by compensation of the reduced grain number per spike through increasing the weight of 1000 grains.
Two methylotrophic strains of Bina coalmine spoil BNV7b and BRV25 were identified based on physiological traits and 16S rDNA sequence as Methylophilus and Methylobacterium species. The strains exhibited similar carbon utilization but differed in N utilization and their response to the metabolic inhibitors. Methylophilus sp. was less tolerant to salt stress and it viability declined to one tenth within 4 h of incubation in 2M NaCl due to membrane damage and leakage of the intracellular electrolytes as evident from malondiaaldehyde (MDA) assay. In 200 mM NaCl, they exhibited increased superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activity while in 500 mM NaCl, enzyme activities declined in Methylophilus sp. and increased in Methylobacterium sp. Among exogenously applied osmoprotectants proline was most efficient; however, polyols (mannitol, sorbitol and glycerol) also supported growth under lethal NaCl concentration.
The hitherto studies on the effect of cadmium on plants have shown that it causes intensification of two types of unfavorable processes in plants: inactivation of macromolecules and cellular structures and induction of oxidative stress. In response, the plant organism activates processes restoring its homeostasis, i.e. those which remove reversible and irreversible changes. In removing the former a particular role is played by the antioxidative system containing enzymatic and nonenzymatic antioxidants, and removing the active forms of oxygen. The strategy of stress tolerance plays an important role in the plant resistance to cadmium and other toxic metals.
Polluted air is a stress factor that contributes to the decline of urban trees. Air pollution may cause short-term (acute) damage, which is immediately visible and long-term (chronic) damage, which can lead to gradual tree decline. Long-term damage may predispose trees to other disorders. The impact of technogenic factors on the leaf’s anatomical structure of Acer negundo L. (Box elder) was studied. The thickness of the upper cuticle is increased when compared to those from an ecologically pure area. A change in the rate of mesophyll tissue is due to the enlargement of the palisade parenchyma. The thickness of lower epidermal cells is decreased. All changes of the leaf blade structure are significant and are in direction of increasing the xerophyte characteristics of the leaves. Box elder is native to much of temperate North America. This is a tree of lowlands and wet hardwood forests. As such, we can assume that the registered changes are adaptive responses of the tree to the contaminated environment and that the tree can be considered to be relatively tolerant.
A pot study was conducted in net house to determine the influence of hormonal priming kinetin (50 mg·dm-3), salicylic acid (50 mg·dm-3), spermine (50 mg·dm-3) and spermidine (50 mg·dm-3) on emergence, yield, antioxidants and ionic content of two wheat (Triticum aestivum L.) cultivars SARC-1 (salt tolerant) and MH-97 (salt sensitive) under normal and saline conditions. The growth of wheat plants was severely affected by salinity stress. But the most of hormonal priming strategies were effective in alleviating the adverse effects of salt stress on wheat plants, however, hormonal priming with salicylic acid (SA) followed by priming with kinetin successfully enhanced seedling vigor by increasing emergence index, decreasing the uptake of Na+ and finally increasing grain yield of both cultivars under normal as well as saline conditions. Activities of SOD and CAT in both cultivars were significantly affected by priming in stressful environment. On the other hand, SARC-1 performed better as compared to MH-97 due to the increased antioxidant activities under salt stress. It can be concluded that priming with 50 mg·dm-3 SA followed by 50 mg·dm-3 kinetin for 12 h maximally improved salt tolerance in both wheat cultivars.
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A factorial experiment was performed to evaluate whether wild and primitive Aegilops and Triticum species may be used in wheat breeding as donors of an improved water use efficiency (WUE) and/or tolerance to nutrient shortage. Seventeen lines representative for Aegilops and Triticum species of different origin, ploidy level and genomic structure were compared with three local cultivars of hexaploid wheat T. aestivum. The genotypes were grown till maturity in experimental pots (9 dm3) under high and reduced NPK nutrition. There was a broad genetic variation in the response to nutrient shortage and efficiency of water use in the vegetative and grain mass formation. The variation was dependent upon species, ploidy level and genome present. Results suggest that a search for enhanced tolerance and novel variation in WUE among wild or primitive wheats may be essential for wheat breeders. The tetraploids T. carthlicum, T. dicoccoid.es and T. timopheevii and the hexaploid T. sphaerococcum were found to be the most promising potential sources of stress tolerance. However, only the primitive T. sphaerococcum appeared to be a valuable donor of improved WUE. Despite a high operative heritability of WUE, testing till plant maturity over diverse levels of soil nutrient status would rather be necessary for a precise discrimination of the most efficient genotypes as indicated by the genotype-stage and genotype-nutrition interactions.
The prediction of species response to human activity is of great interest in contemporary restoration ecology. The purpose of the article was to analyse which species life history traits and species habitat preferences are important during succession after the abandonment of mining activity in a sand-pit. During a 15-year period (1996–2010), 176 phytosociological relevés were placed within vegetation patches of different ages and divided according to soil moisture, thus forming two series of chronosequences that ranged from 0 to ca. 50 years. The datasets were analysed using both DCA/CCA ordinations and regression trees. The successional gradient, apart from the theoretically predicted replacement R by C strategists, revealed the occurrence of stresstolerant ruderals and competitive ruderals in the early successional stages. However, differences were observed between the wet and dry series. On dry soils the ruderals, anemochorous and windpollinated species dominated in the early phases. In the middle successional phases, a preponderance of anemochorous species (nanophanerophytes, light or semi-shade demanding species), nitrogen-poor and competitive ruderals or species typical for nitrogen-rich soils was recorded. In the late successional stages, species with both the ability of vegetative and generative reproduction appeared. On the other hand, on wet sites in the early successional phases, species with vegetative growth, hydrophytes, chamaephytes and stresscompetitors prevailed. Later in the succession, they were replaced by insect-pollinated species in nitrogen rich habitats and stress-tolerant ruderals on less fertile habitats. Finally, competitors started to prevail. Knowledge of the environmental conditions of a given site, the ecological processes and species biology can assist in achieving the desired goals or in initiating or enhancing succession on some disturbed sites.
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