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The experiments were carried out on mice (Balb/c, 6 weeks old) exposed to restraint stress. Animals were restrained for 12 h per day at nighttime and released at daytime for 2 consecutive days. Some mice were immunized i.p. immediately before the stress with 4xl08 sheep red blood cell (SRBC). Sodium diethyldithiocarbamate (DTC, 20 mg/kg) was administered i.p. twice e.g. 4 and 2 days prior to restraint stress. Calf thymus extract (TFX, 10 mg/kg) was injected i.p. four times at 24 h intervals prior to exposure to stress. It was found that restraint stress led to thymic atrophy which was reflected in the decreased total number of thymocytes, weight index of the thymus, and caused depletion of thymocytes. In addition, it was found that restraint stress reduced humoral response to SRBC which was reflected in the decreased number of splenocytes producing anti-SRBC antibodies (PFC) and serum haemagglutynin titres (19S+7S and 7S). The total number of spleen cells and weight index of the spleen in stressed mice were also diminished. The suppressing effect of stress was observed for 10 days. Pretreatment with DTC or TFX partially counteracted the immunosuppresive effects of restraint stress. Administration of DTC or TFX retarded the stress-induced thymic atrophy and promoted the restoration of the synthesis of anti-SRBC haemagglutinins and the number of PFC. Regeneration of the thymus gland occured more rapidly in stressed mice previously treated with TFX. On the other hand, the stronger effect of restoring the humoral response to SRBC was observed for DTC.
The studies were done on 50 male Wistar rats divided into five groups: 1 - controls, II - rats immobilized for 13 hr, III - animals immobilized after peritoneal infection with a pathogenic E. coli, IV - rats immobilized after the application of vitamin E (30 mg/kg bw) for six continous days, V - rats immobilized after the application of vitamin E and peritoneal injection of a pathogenic E. coli. Metabolic neutrophil activity (NBT-test) was determined spectrophotometrically in blood and the level of corticosterone was estimated in blood plasma by the radiocompetition method. It was found that an increase of corticosterone and decrease of metabolic neutrophil activity appeared in rats after immobilization (group II). However, a significant decrease of neutrophil activity and an inconsiderable increase of corticosterone were noted in group III and V. Immobilization of rats after the administration of witamin E did not affect the level of the examined parameters (group IV).
In 15 pigs subjected to the stress of isolation, immobilization and slaughter, the c-reactive protein, ceruloplasmine, total protein level, gamma-globulin fractions, as well as lysozyme activity in the sera were tested. It was determined that the stres connected with slaughter does not effect changes in the concentration of acute phase protein (APP), while total protein levels as well as gamma globulin fractions are lowered both during immobilization and stress. Lysozyme activity in the sera rises during the isolation of the pigs, which may indicate the mobilization of uncharacteristic defence mechanisms.
The level of adrenaline, noradrenaline and corticosterone in blood plasma of adult chickens immobilized by fastening of legs and wings was examined after 1, 3, 6, 12 and 24 h since the beginning of immobilization. The level of adrenaline and corticosterone increased significantly after 3 and 6 h since the beginning of immobilization and then steadily decreased. The concentration of noradrenaline significantly lowered during the whole period of immobilization. It was also noted a slight increase of metabolic activity of blood neutrophils measured in the NBT. These results point to decrease of the suprarenal glands activity (adaptation) just after a few hours of stress.
The studies were performed on 36 broiler chickens in 3 experimental groups, 12 animals of each; group I — control, group II and III — chckens immobilized everyday for 5 h. Moreover in the IIIrd group at the day 15 of the experiment after 2 h immobilization chickens were dipped into a water bath (16°C) for 15 min. with 1 min. breaks after each 5 minutes. In blood obtained by decapitation the following parameters were determined: level of adrenaline, noradrenaline and corticosterone, metabolic activity of neutrophiles. It was found that immobilization induces in chickens a significant increase of blood adrenaline, noradrenaline and corticosterone level. A new type of the stressor used, water bathing, increased further significantly the level of the examined hormones. The used stressors did not affect metabolic activity of neutrophiles.
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