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Beta-glucuronidase and beta-glucosidase activity in stool specimens of children with inflammatory bowel disease

100%

Mroczynska M., Galecka M., Szachta P., Kamoda D., Libudzisz Z., Roszak D.

Polish Journal of Microbiology

2013

tom 62

nr 3

The aim of the study was to analyze the differences in the activity of β-glucuronidase and β-glucosidase in stool specimens of children with Inflammatory Bowel Diseases (IBD) and healthy subjects. The disease activity was determined according to the PCDAI scale (Crohn disease) and Truelove-Witts scale (Ulcerative colitis). Enzyme activity was determined by spectrophotometry. There was a correlation between the level of β - glucosidase activity in stool and patient’s age in the group of healthy controls, but not in the IBD group. β-glucosidase activity in IBD and healthy subjects stool specimens did not differ significantly. The activity of β-glucuronidase in children with IBD was two times lower than in the healthy group and was correlated with age in children with IBD, but not in the group of healthy ones.

Detection of Clostridium difficile and its toxin A [TcdA] in stool specimens from hospitalised patients

100%

Wroblewska M. M., Swoboda-Kopec E., Rokosz A., Nurzynska G., Bednarska A., Luczak M.

Polish Journal of Microbiology

2005

tom 54

nr 2

The study has been carried out to determine the frequency of C. difficile recovery in stool cultures and the rate of C. difficile toxin A detection in faecal specimens of patients with nosocomial diarrhoea. Clinical specimens comprised 4414 stool samples collected from 1998 to 2002 from adult patients hospitalised in different wards of a university-affiliated hospital (1200 beds) and suspected of C. difficile-associated disease (CDAD). There have been 1308 (29.6%) specimens positive for C. difficile culture (15.1% in 1998, 29.5% in 1999, 33.8% in 2000, 31.2% in 2001 and 32.0% in 2002). The highest number of C. difficile strains was cultured from stool samples of patients hospitalised in the haematology/oncology ward (51.1% of all isolates), neurology (8.3%), nephrology (8.0%), gastrointestinal surgery (7.0%) and neurosurgery (6.2%) wards. The testing for C. difficile toxin A yielded 847 (19.2%) positive samples and 3567 (80.8%) toxin A-negative results. The percentage of C. difficile toxin A-positive samples was 29.4% in 1998, 17.5% in 1999, 23.2% in 2000, 17.1% in 2001 and 15.0% in 2002. In the analysed period we observed an increase in the number of stool specimens tested for C. difficile and an increase in the number of C. difficile culture-positive samples. A decrease in the number of C. difficile toxin A-positive samples was noted in the last 2 years of the study. This phenomenon may be due to an improved antibiotic policy of the hospital.

Ograniczanie wyników

2 Polish Journal of Microbiology

1 Bednarska A.

1 Galecka M.

1 Kamoda D.

1 Libudzisz Z.

1 Luczak M.

1 Mroczynska M.

1 Nurzynska G.

1 Rokosz A.

1 Roszak D.

1 Swoboda-Kopec E.

1 Szachta P.

1 Wroblewska M. M.

1 2013

1 2005

Wyniki wyszukiwania

Beta-glucuronidase and beta-glucosidase activity in stool specimens of children with inflammatory bowel disease

Detection of Clostridium difficile and its toxin A [TcdA] in stool specimens from hospitalised patients