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The aim of the research was to monitor the influence of neonatal thymectomy on the reactivity of chicken spleen structures. The investigations were carried out on chickens with the thymus removed on the 1st day after hatching and with antigen administered in the form of sheep erythrocytes (SRBC) at the age of 12 weeks. On days 6, 14 and 21 after antigen administration the spleen was taken out and histological slides were prepared. On the established area of HE slides germinal centres were counted and mature centres of type I and immature ones of type II were distinguished. Reaction to the acid phosphatase (APh) was done by the Gomori method. The results of the reaction was read on the established area within the periarterial lymphatic tissue (PAL) using the Thomson point method. It was found that neonatal thymectomy leads to a decrease in the number of germinal centres and simultaneously to the reduction of the intensity of APh reaction within the spleen PAL. Following immunization of the thymectomized chickens, in comparison to the control (group) a change of kinetics of germinal centers formation was discovered, mainly of type I. It was ascertained that in the succeeding days after the antigen administration the intensity of APh reaction within PAL was directly proportional to the number of immature germinal centres of type II. The results demonstrated a distinct association between the bursodependent and thymodependent spleen structures reactivity and confirmed the importance of the functional state of the thymus.
The experiments were performed on 3 sheep which had bi-polar platinic electrodes subserously implanted to the uterus shank and horns during an operation. The registration of uterus myoelectrical activity was conducted by the use of Reega Duplex XVI electroencephalograph. The tests were carried out on sheep in uterus, sensitised and non-sensitised with Stilboestrol-Polfa (0.04 mg kg-1 i.m.). After recording the output uterus activity, the sheep were administered serotonin in continuous infusion in the quantity of: 12-24 g kg-1 min-1. The obtained results revealed that serotonin in sheep in anestrus, non-sensitised with stilboestrol, does not cause (within the scope of action potentials) any changes in the electrouterographic record. In sheep sensitised with stilboestrol, serotonin increases the uterus myoelectrical activity.
Experiments were carried out on seven anestrus ewes. The animals were implanted with bipolar electrodes in the horns and corpus uteri. The electrical activity was registered by Reega Duplex electroencephalograph. The experiments were carried out on non-sensitized ewes and ewes sensitized by stilboestrol. The sheep were first fitted with an external jugular vein catheter and histamine (Histaminům hydrochloricum - Polfa) - 3 μg . kg-1 • min-1 - or H1 -receptors blocker (Clemastinum-Polfa) - 50 μg • kg-1 - were introduced. It was found that in sheep sensitized by stilboestrol histamine caused an increase in myoelectrical activity of the uterus by stimulation of H1 - histaminergic receptors.
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