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1

Sterols in fruits and leaves from Pyracantha coccinea [L.] Roem

100%

Sokolowska-Wozniak A., Sadlak-Galewska M., Gawdzik J., Mardarowicz M.

Herba Polonica

|

2004

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tom 50

|

nr 3-4

The fraction of sterols acetates from fruits and leaves of P. coccínea have been isolated in a typical way from a petroleum ether extract. Besides, by means of the weight method the total amounts of sterols were determined in fruits and leaves (148.2 mg and 75.36 mg per 100 g, respectively), ß-sitosterol, cholesterol, stigmasterol and campesteryl were identified in both fractions by GC, GC/MS and TLC techniques, ß-sitosterol was the predominant sterol component in analysed raw materials obtained from P. coccínea.

2

Sterols in the roots and herb from Hieracium pilosella L.

88%

Krzaczek T., Lukasiewicz E., Gawronska-Grzywacz M.

Herba Polonica

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2002

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tom 48

|

nr 4

A comparative study of the sterol components of roots and herb of H. pilosella was carried out using GCMS techniques, β-sitosterol, stigmasterol, campesterol and α-saccharostenon were identified in both fractions. Stigmast-7-en-3-ol was identified only in herb and stigmast-4-en-3-on only in roots. Sitosterol and stigmasterol were the predominant sterol components in analysed raw materials. Besides, by means of the weight method the total amounts of sterols was determined: in herb 0.24% and in roots 0.16%.

3

Sterols in leaves and roots of Jovibarba sobolifera [Sims.] Opiz [Hen and Chickens Houseleek]

88%

Szewczyk K., Krzaczek T.

Herba Polonica

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2004

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tom 50

|

nr 1

GC MS methods were used to analyse sterol components of leaves and roots of Jovibarba sobolifera (Sims.) Opiz. ß-sitosterol, campesterol, 24-ethylcholestanol, 5ot-ergostan-3a-ol, -y-ergosteryl and 5a-stigmast-7-en-3a-ol have been identified in both fractions, ß-sitosterol and campesterol have been the predominant sterol components in the analysed leaves, whereas ß-sitosterol and 24-ethylcholestanol have been the main sterol components in the roots. Besides, by means of the weight method a total amount of sterols has been determined: 0.04% in leaves and 0.02% in roots.

4

Fenugreek as a source of plant sterols

75%

Rudzinska M., Przybylski R., Ciftci O. N.

Polish Journal of Food and Nutrition Sciences

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2011

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tom 61

|

nr Suppl.1

5

Sterol and acids of horseradish roots [Cochlearia armoracia L.] - short report

75%

Kroszczynski W., Tretowska J., Bal K.

Annals of Warsaw Agricultural University. Horticulture

|

1999

|

tom 20

6

Sterols and fatty acids in the seeds of roselle [Hibiscus sabdariffa L.] cultivated in Egypt

75%

Kosakowska O., Weglarz Z., Sidky M., Przybyl J., Geszprych A.

Herba Polonica

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2005

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tom 51

|

nr 1-2

The comparative study on the composition of fatty acids and sterols in the seed oil of four forms of Roselle (Hibiscus sabdariffa L.) cultivated in Egypt was carried out. The total oil content in the seeds of the investigated forms ranged from 15.31 to 18.99?á. As a result of the GC analysis five fatty acids were identified: palmitic, stearic, linolic, y-linoleic, and a-linoleic acid. The forms differed both in respect of the presence and the content of particular fatty acids. The HPLC analysis indicated the presence of four free sterols in the Roselle seed oil: brassicasterol, campesterol, stigmasterol and sitosterol. Sitosterol appeared to be the main sterol and its content depended on the form of Roselle.

7

Sterols in the roots and fruits from Angelica sylvestris L.

75%

Krzaczek T., Nowak R.

Herba Polonica

|

2000

|

tom 46

|

nr 4

Metodą GCMS przeprowadzono porównawcze badania obecności steroli w korzeniach i owocach A. sylvestris. W obu badanych frakcjach zidentyfikowano sitosterol, stigmasterol, kampeste- rol, cholesterol oraz stigmast-7-en-3-ol. Ergosta-5,24-dien-3a-ol i a-saccharostenon stwierdzono tylko w owocach. Sitosterol i stigmasterol byly związkami dominującymi w obu surowcach. Metodą wagową określono całkowitą ilość steroli w owocach (0,12%), w korzeniach (0,06%), a także ilość oleju w owocach (12,6%).

8

Immunological functions of steryl glycosides

63%

Shimamura M.

Archivum Immunologiae et Therapiae Experimentalis

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2012

|

tom 60

|

nr 5

9

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Wydzielanie fitosteroli z oleju talowego lub paku oleju talowego

63%

Jakubowski A., Braczko M.

Rośliny Oleiste - Oilseed Crops

|

2000

|

tom 21

|

nr 1

187-213

10

Characterization of bioactive substances and composition of selected nuts available on Polish market

63%

Derewiaka D., Welenc A., Druzynska B., Ciecierska M., Majewska E., Kowalska J., Wolosiak R.

Towaroznawcze Problemy Jakości. Polskie Towarzystwo Towaroznawcze

|

2017

|

nr 2

11

Sterole i ich utlenione pochodne w olejach roslinnych rafinowanych i tloczonych na zimno.

63%

Rudzinska M., Kazus T., Wasowicz E.

Rośliny Oleiste - Oilseed Crops

|

2001

|

tom 22

|

nr 2

477-494

12

Intraspecific variability of great burnet (Sanguisorba officinalis L.) in respect of sterols content

63%

Mirgos M., Przybyszewska E., Capecka E., Angielczyk M., Przybyl J. L., Baczek K., Weglarz Z.

Herba Polonica

|

2012

|

tom 58

|

nr 3

The content and composition of sterol compounds in wild growing great burnet (Sanguisorba officinalis L.) populations were determined. The herb and underground organs of this plant were collected from 10 natural sites in Poland. The HPLC analysis revealed the presence of 5 sterol compounds, namely β-sitosterol, β-sitosterol D-glucoside, campesterol, brassicasterol and stigmasterol in these raw materials. Among them, β-sitosterol Dglucoside was dominant one. The herb was richer in sterol compounds than underground organs. There were significant differences between the content of above mentioned compounds in these populations.

13

Accumulation of phenolic and sterol compounds in Euphorbia hirta (L.)

63%

Pioro-Jabrucka E., Pawelczak A., Przybyl J. L., Baczek K., Weglarz Z.

Herba Polonica

|

2011

|

tom 57

|

nr 2

Euphorbia hirta is an annual plant grownig commonly in the tropical and subtropical regions of Asia, Africa as well as Central and South America. Extracts from herb reveal diastolic, anti-inflammatory, diuretic and anthelminthic effects. Biologically active compounds of this plant are sterols, saponins, flavonoids and phenolic acids. The results of this study indicate that obtaining relatively high yield of herb and well germinating seeds which retain their germinability after two years of storage is possible in Poladn. Chemical analysis of the herb and callus obtained in vitro has shown that these raw materials accumulate phenolic compounds and sterols in high quantities.

14

Secondary metabolites in cell culture of clary sage [Salvia sclarea L.]

63%

Kuzma L., Skrzypek Z., Wysokinska H.

Herba Polonica

|

2004

|

tom 50

|

nr 3-4

Suspension culture was established from the roots of Salvia sclarea L. The maximum suspension cell growth was 14.3 g/flasl< (fresh mass) and 0.9 g/flasl< (dry mass), on the 18"' and 15"' day after inoculation, respectively. The isolation and characterisation of abietane type diterpenoids and phenolics, including flavones and rosmarinic acid from the cell culture was reported. A GC-MS analysis also showed the presence of pentacyclic triterpenoids and sterols in in vitro cultured cells of S. sclarea.

15

Sterols in the herb and rhizomes from Comarum palustre L.

63%

Sokolowska-Wozniak A., Krzaczek T., Bialopiotrowicz L., Gawdzik J., Mardarowicz M.

Herba Polonica

|

2002

|

tom 48

|

nr 4

The fraction of sterols acetates from the herb and rhizomes from C. palustre have been isolated in the typical way from petroleum ether extract. By means of the weight method the total amounts of sterols was determined in herb (0.1192%), in rhizomes (0.0517%). The mixture of sterols acetates was investigated by capillary gas chromatography GC MS techniques. Stigmasterol, β-sitosterol, campesterol and ergosterol were identified in both fractions. Stigmasterol and β-sitosterol were the predominant sterol components in analysed raw materials from C. palustre.

16

Metabolism of conjugated sterols in eggplant. Part 1. UDP-glucose: sterol glucosyltransferase

63%

Potocka A., Zimowski J.

Acta Biochimica Polonica

|

2008

|

tom 55

|

nr 1

A membrane-bound UDP-glucose:sterol glucosyltransferase from Solanum melongena (eggplant) leaves was partially purified and its specificity as well as molecular and kinetic properties were defined. Among a wide spectrum of 3-OH steroids (i.e. typical plant sterols, androstane, pregnane and cholestane derivatives, steroidal alkaloids and sapogenins) and triterpenic alcohols, the highest activity was found with 22-oxycholesterol. UDP-glucose appeared to be the best sugar donor. The enzyme preparation was also able to utilize UDP-galactose, TDP-glucose and CDP-glucose as a sugar source for sterol glucosylation, however, at distinctly lower rates. The investigated glucosyltrasferase was stimulated by 2-mercaptoethanol, Triton X-100 and negatively charged phospholipids, and inhibited in the presence of UDP, mono-, di- and triacylglycerols, divalent cations such as Zn 2+, Co 2+, high ionic strength, cholesteryl glucoside, galactoside and xyloside and some amino acid-modifying reagents (SITS, DIDS, PLP, DEPC, pCMBS, NEM, WRK and HNB). Our results suggest that unmodified residues of lysine, tryptophan, cysteine, histidine and dicarboxylic amino acids are essential for full enzymatic activity and indicate that a glutamic (or aspartic) acid residue is necessary for the binding of sugar donor, i.e. UDP-glucose in the active site of the GT-ase while histidine and cysteine residues are both important for the binding of the nucleotide-sugar as well as of the steroidal aglycone.

17

Sterols and fatty acids in the seeds of evening primrose [Oenothera sp.] and willow herb [Epilobium sp.]

63%

Pelc M., Kosakowska O., Weglarz Z., Przybyl J., Geszprych A.

Herba Polonica

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2005

|

tom 51

|

nr 3-4

The content and composition of fatty acids and sterols in the seed oil of two cultivated evening primrose species (Oenothera paradoxa Hudziok, 0. glazioviana Micheli in Mart.) and two willow herb species (Epilobium tetragonum L., E. hirsutum L.) were compared. A GC analysis of the evening primrose seed oil resulted in identifying five fatty acids (palmitic, stearic, oleic, linoleic, and y-linolenic acids), with linoleic and y-linolenic acids as dominant compounds. In the willow herb seed oil six fatty acids were identified (palmitic, stearic, oleic, linoleic, y-linolenic and a-linolenic acids) with palmitic and linoleic acids as dominant compounds. An HPLC analysis indicated the presence of four free sterols in the evening primrose seed oil and five in the willow herb seed oil. ß-sitosterol and brassicast- erol appeared to be the main sterols in seed oils of the investigated species.

18

Metabolism of conjugated sterols in eggplant. Part 2. Phospholipid: steryl glucoside acyltransferase

63%

Potocka A., Zimowski J.

Acta Biochimica Polonica

|

2008

|

tom 55

|

nr 1

A membrane-bound phospholipid:steryl glucoside acyltransferase from Solanum melongena leaves was partially purified and its specificity and molecular as well as kinetic properties were defined. Among the steryl glycosides tested (e.g. typical plant steryl glucosides, steryl galactosides and cholesteryl xyloside) the highest activity was found with cholesteryl glucoside, but some structurally related compounds such as sito- and stigmasteryl glucoside or galactoside as well as cholesteryl galactoside were also acylated, albeit at lower rates. The investigated enzyme was able to use all classes of phosphoglycerolipids (phosphatidylethanolamine, phosphatidylcholine, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol) as an acyl source for biosynthesis of acylated steryl glucoside. Among them 1,2-dimirystoylphosphatidylic acid appeared to be the best acyl donor. Apart from phosphoglycerolipids, 1,2-diacylglycerols were also used as acyl donor for steryl glucoside acylation, although at a distinctly lower rate. The acyl moiety was transferred from the C-1 position of phospholipid molecule. The investigated acyltrasferase activity was stimulated by 2-mercaptoethanol, Triton X-100, 1-monoacylglycerols and inhibited in the presence of divalent cations such as Ca 2+, Mn 2+, Zn 2+ or Co 2+, some lipids (MDGD, ceramide), detergents (Tween 20, 40, 60 and 80, Tyloxapol, sodium deoxycholate) and high ionic strength.

19

GC-MS determination of sterols from Rheum species cultivated in Poland

63%

Medynska E., Smolarz H. D.

Herba Polonica

|

2004

|

tom 50

|

nr 3-4

The sterols in petrol extracts from the underground part and petioles of Rheum rhaponticum L. and Rheum undulatum L., as well as from the underground part of Rheum palmatum L. were determined by the GC-MS method. The study enabled detecting in all the samples the following sterols, as sterol acetates: cholesteryl, (3ß,24R)-ergost-5-en-3ol, (3ß,22Z)-stigmasta-5,22-dien-3ol, and ß-sitosterol. The investigated species contain the same sterols, detected in similar amounts, while ß-sitosterol is the main component of the sterol fraction of the species Rheum cultivated in Poland. Besides, vitamin E were detected in investigated species.

20

Molecular modelling study of the role of cholesterol in the stimulation of the oxytocin receptor

63%

Politowska E., Kazmierkiewicz R., Wiegand V., Fahrenholz F., Ciarkowski J.

Acta Biochimica Polonica

|

2001

|

tom 48

|

nr 1

Cholesterol, an integral component of membranes in Eucaryota, is a modifier of membrane properties. In vivo studies have demonstrated that cholesterol can also modulate activities of some G protein-coupled receptors (GPCRs), which are integral membrane proteins. This can result either from an effect of cholesterol on the membrane fluidity or from specific interactions of the membrane cholesterol with the receptor, as recently demonstrated for the cholecystokinin type β (CCKRβ ) or the oxytocin receptor (OTR). Using molecular modelling, we studied conformational preferences of cholesterol and several of its analogues. Subsequently, we simulated the distributions of their preferred conformations around the surface of OTR, CCKR β and a chimeric oxytocin/cholecystokinin receptor. Consequently, we suggest residues on the surface of OTR which are potentially significant in the OTR/cholesterol interaction.

Ograniczanie wyników

Sterols in fruits and leaves from Pyracantha coccinea [L.] Roem

Sterols in the roots and herb from Hieracium pilosella L.

Sterols in leaves and roots of Jovibarba sobolifera [Sims.] Opiz [Hen and Chickens Houseleek]

Fenugreek as a source of plant sterols

Sterol and acids of horseradish roots [Cochlearia armoracia L.] - short report

Sterols and fatty acids in the seeds of roselle [Hibiscus sabdariffa L.] cultivated in Egypt

Sterols in the roots and fruits from Angelica sylvestris L.

Immunological functions of steryl glycosides