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The interaction of epibrassinolide (epiBL) with gibberellins (GA3 and GA4+7) in tulip stem growth were studied. When stem length was about 10.0 cm, excision of all leaves and flower bud almost fully inhibited the stem growth in tulips. Epibrassinolide (epiBL) applied at a concentration of 100 nM or 300 nM did not induce tulip stem growth. The application of GA3 or GA4+7 at the concentration of 250 mg·dm-3 alone at all the internodes, after excision of all leaves and flower bud, induced slightly the increase length of all internodes. GA4+7 stimulated stem growth more than GA3. The application of gibberellins in the mixture with epiBL stimulated the growth of tulip shoot much more than gibberellins alone.
In recent years extensive attention has been given to the Rubus genus, but knowledge on the ecology of most of its species, including rhythmic development, is still insuffi cient. Such data may have practical applications, since blackberry species are economically important. The goal of the study was to analyse phenology and growth rate of vegetative and generative stems in 10 Rubus species of different systematic affi nity (belonging to two subspecies and three sections), growing in the Dendrological Garden of the Poznań University of Life Sciences. Some habitat factors were also taken into consideration. Field investigations were carried out for three growing seasons. The plants were evaluated for phenology twice a week, while the length of their stems was measured once a week. Diff erences were observed in the seasonal rhythm of individual species. Weather factors distinctly infl uenced the course of development phases in all the Rubus species. Drought during the growing season caused fl owering disorders, withering of fruit, earlier autumn leaf coloration, and fall of leaves. Rubus idaeus and R. fabrimontanus ripened all of their fruits each year of observations and seemed to be the most adapted for fruit production. Rubus fabrimontanus, R. kuleszae and R. praecox were the most vigorous species with reference to shoot growth. This study revealed that only some species were fully acclimated to the conditions of the Dendrological Garden, since they successfully completed their entire fl owering and fruiting cycles.
The interaction of epibrassinolide (epi-BL) with auxin in tulip stem growth and ethylene production were studied. Excision of all leaves and flower bud in isolated shoots (about 5 cm long) of tulip almost totally inhibited stem growth. IAA at both concentrations (0.1% and 1.0%) greatly induced the growth of tulip shoot, but higher concentration at IAA in smaller degree stimulated the growth. Epibrassinolide at concentration 0.05 µM applied simultaneously with auxin did not affect tulip stem growth induced by IAA treatment alone. However, higher concentration of epi-BL (1.0 µM) stimulated tulip stem growth induced by IAA at both concentrations. IAA at both concentrations stimulated ethylene production in the stem internodes of tulips. Higher concentration of auxin stimulated ethylene production more than low concentration. Epibrassinolide applied simultaneously with auxin evidently enhanced ethylene production measured 3 and 5 days after treatment in comparison to IAA treatment alone.
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