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Bioparameters of selected herb species were studied on five control (undamaged) and five bark beetles damaged parallel plots established in form of two vertical transects in the spruce ecosystems of Bielovodská valley (High Tatra Mts.). The most abundant life forms in the studied phytocoenoses were hemicryptophytes (65%), the least abundant geophytes (5%). According to significance of differences in the average length of shoots the sensitivity of herb species to different ecological conditions and calamitous changes of the spruce stands density is as follows: Homogyne alpina > Luzula sylvatica = Dryopteris dilatata > Oxalis acetosella. According to significance of differences in energy content the sensitivity of species was following: Luzula sylvatica > Oxalis acetosella > Dryopteris dilatata > Homogyne alpina > Vaccinium myrtillus. However, the mean values calculated for undamaged and for damaged plots were significantly different (P <0.05) only in the case of weight of Luzula sylvatica shoots.
Selection of nest sites by the common dormouse Muscardinus avellanarius L. depends on habitat type and suitable plants that can support and hide dormouse nests properly. Lithuania is situated in the northern part of the distribution rage of M. avellanarius. Some peculiarities of nest site selection in these dormice could be expected here compared to the regions situated further south because of differences in composition of the woody vegetation. Searches for nests of M. avellanarius were carried out in different young woodlands of Lithuania at 16 study sites, and detailed study of nest site selection in this species was carried out in an overgrown clearing in 2005–2006. In comparison to other parts of its distribution range, significant prevalence of Norway spruce as a nest supporting plant was observed in Lithuania. More than 70% of nests of M. avellanarius (n = 120) found in different young woodlands were situated in young spruce trees. In habitats where suitable spruce trees were absent or scarce, young deciduous tress (e.g. oak, ash, aspen, lime, hornbeam) and shrubs (e.g. hazel, bramble, raspberry, willow, honeysuckle) were selected for nesting sites. In overgrown mid-forest clearing, young spruce trees were evidently preferred by M. avellanarius as nest supporting plants despite their comparatively scarcity. Planted oak trees were selected by M. avellanarius for nesting in the plot of the clearing where young spruce trees were almost absent. The average height of dormouse nests was 1.0 ± 0.6 m above ground in young woodlands, and it was related to the age and height of young trees and shrubs.
The aim of this study was to analyse the frequency and extent of mechanical damage of tree stems in mountain spruce stands of varying forest cover index and ground slope, without taking into account the technology used in obtaining wood. The study was conducted in 17 spruce stands located in the Sudetes and the Carpathians. In the stands the 1 ha test surfaces were established. The damage on trees with dbh ≥ 18 cm was measured. As a result of the analysis it was found that the most damaged trees grew in the stand with forest cover ratio of 0.7 and 1.0, while taking into account the decline of the area most damage was found in the stands with a slope of 16 to 25°. The study, however, showed no clear relationship between the position of necrosis on tree stems (its size and height) and forest cover and a decrease of the area.
The paper provides some guidelines on the choice of the target species compositions for plantations that would replace monocultural spruce stands growing on the sites of the Silesian Beskid Mts. Part of the guidelines relates to the rules governing the sequence in which the monocultures will be converted into mixed stands. It is suggested that those rules should be combined with the principles of the establishment of progenyplantations. The seed stands of the Istebna spruce are experimentally divided into four categories according to their abilityto perform the function of a seed stand. The following features of the stands are analysed: vitality, density, stage of regeneration, protective and cultural functions. The paper contains also an outline of the methods of species conversion and the relevant references.
The study analyses the transfer of F-, Cl-, NO3-, SO42-, Na+, K+, Ca2+, Mg2+, Fe2+, Mn2+ and Zn2+ from bulkprecipitation to throughfall, soil surface flow, vertical flow and intercover flow of water (gravity lysimeters at 20 cm soil depth) in spruce stands of different age classes (1st, 2nd, 5th, 6th), and the amount of these ions flowing out from the Potok Dupniański catchment in the Silesian Beskid Mts. The results cover the year 2000. The concentration and amount of SO4 2- in throughfall systematically increased and the pH value decreased with the age of the stands and with successive elements of the ecosystem. The NH4+ ion was probably absorbed in the canopy, which caused a washout of K+, Mn2+, F- and SO42- in the vegetation season in older stands. In the winter season, Fe2+, Mn2+, Na+ and K+ were washed out from the youngest stands (1st age class), whereas F-, NO3 -, SO42--, K+, Ca2+, Mg2+, Mn2+ and Fe2+ – from older canopies. Decomposition of the organic matter caused a rise in water acidity and an increase in the concentrations of all the analysed ions; their leaching, however, was low (under 1%). Water from intercover flow contributed to an increase in the amount of water and the concentration and amount of ions and to a further decrease in water reaction at a soil depth of 20 cm. All the anions and cations flowed away with water penetrating vertically and horizontally. Considerable amounts of ions, especially SO42-, Na+, K+, Ca2+ and Mg2+, were carried beyond the reach of the main mass of the root system and then out of the catchment, which may adversely affect the development and health of spruce stands.
Height-diameter models define the general relationship between the tree height and diameter at each growth stage of the forest stand. This paper presents generalized height-diameter models for mixed-species forest stands consisting of Norway spruce (Picea abies Karst.), Silver fir (Abies alba L.), and European beech (Fagus sylvatica L.) from Slovakia. The models were derived using two growth functions from the exponential family: the two-parameter Michailoff and three-parameter Korf functions. Generalized height-diameter functions must normally be constrained to pass through the mean stand diameter and height, and then the final growth model has only one or two parameters to be estimated. These “free” parameters are then expressed over the quadratic mean diameter, height and stand age and the final mathematical form of the model is obtained. The study material included 50 long-term experimental plots located in the Western Carpathians. The plots were established 40–50 years ago and have been repeatedly measured at 5 to 10-year intervals. The dataset includes 7,950 height measurements of spruce, 21,661 of fir and 5,794 of beech. As many as 9 regression models were derived for each species. Although the “goodness of fit” of all models showed that they were generally well suited for the data, the best results were obtained for silver fir. The coefficient of determination ranged from 0.946 to 0.948, RMSE (m) was in the interval 1.94–1.97 and the bias (m) was –0.031 to 0.063. Although slightly imprecise parameter estimation was established for spruce, the estimations of the regression parameters obtained for beech were quite less precise. The coefficient of determination for beech was 0.854–0.860, RMSE (m) 2.67–2.72, and the bias (m) ranged from –0.144 to –0.056. The majority of models using Korf’s formula produced slightly better estimations than Michailoff’s, and it proved immaterial which estimated parameter was fixed and which parameters were free.
Embryogenic cultures of plants are exposed to various stress factors both in vitro and during cryostorage. In order to safely include the plant material obtained by somatic embryogenesis in combination with cryopreservation for breeding programs, it is necessary to monitor its genetic stability. The aim of the present study was the assessment of somaclonal variation in plant material obtained from embryogenic cultures of Picea abies (L.) Karst. and P. omorika (Pančić) Purk. maintained in vitro or stored in liquid nitrogen by the pregrowth-dehydration method. The analysis of genetic conformity with using microsatellite markers was performed on cotyledonary somatic embryos (CSE), germinating somatic embryos (GSE) and somatic seedlings (SS), obtained from tissues maintained in vitro or from recovered embryogenic tissues (ETc) and CSE obtained after cryopreservation. The analysis revealed changes in the DNA of somatic embryogenesis-derived plant material of both Picea spp. They were found in plant material from 8 out of 10 tested embryogenic lines of P. abies and in 10 out of 19 embryogenic lines of P. omorika after in vitro culture. Changes were also detected in plant material obtained after cryopreservation. Somaclonal variation was observed in ETc and CSE of P. omorika and at ETv stage of P. abies. However, most of the changes were induced at the stage of somatic embryogenesis initiation. These results confirm the need for monitoring the genetic stability of plants obtained by somatic embryogenesis and after cryopreservation for both spruce species.
The regeneration of callus was conducted on of Norway spruce (Picea abies (L.) Karst.) cotyledons from 5 to 19 days old seedlings. The best results (55% on average) were obtained from 5-days old seedlings. The highest percent of regeneration was ascertained on the 1/2 SH medium (on average for all ages 49.17%). The best medium for 5-days old seedlings turned out to be the 1/2 LP (77,50%), but the regeneration ability of cotyledons taken from older seedlings declined rapidly in that medium. On a medium with the concentration of both micro- and macroelements reduced to 50% of the fall level the average regeneration rate was 41.50%, while on a medium with full concentration of nutrients the regeneration amounted only to 25 75%. The best growth of callus after 6 weeks of cultivation was found on cotyledons taken from 5-days old seedlings on the 1/2 LM, MS arid 1/2 LP medium (the diameter of callus equal to 2.95, 2.86 and 2.21 mm respectively). Callus growing on medium with lower concentration of nutrients after 6 weeks of cultivation gave larger callus than that on medium with a full nutrient concentration {average diameters 1.86 mm and 1.52 mm, respectively).
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