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Alternaria spores are known to be potent aeroallergens and their concentrations in the air are strongly dependent on meteorological factors. There are many articles from different parts of the world about relationships between Alternaria spore count and weather parameters. The aim of the study was to review all available publications about airborne Alternaria spores and compare the results in short, useful form.
A multiple linear regression analyses were performed to screen for the significant factors simultaneously influencing production of delta-endotoxin, proteolytic activities and spore formation by a Bacillus thuringiensis kurstaki strain. Investigated factors included: pH of the medium, available oxygen and inoculum size. It was observed that oxygen availability was the most influencing setting on both deltaendotoxins production and spores counts, followed by initial pH of the medium and inoculum size. On other hand, pH of medium was found to be the most significant parameter for proteolytic activity, followed by inoculum size and dissolved oxygen. Our results suggested that the first order with two-factor interaction model seemed to be more satisfactory than simple first order model for optimization of delta-endotoxin overproduction. The coefficients of determination (R²) indicated a better adequacy of the second order models to justify the obtained data. Based on results, relationships between delta-endotoxins production, proteolytic activities and spores counts were established. Our results can help to balance delta-endotoxins production and its stability.
Two new species of sphenophyllalean strobili with in situ spores are proposed from the Radnice Basin of the western and central Bohemian Carboniferous continental basins of the Czech Republic. Bowmanites brasensis sp. nov. from Břasy (Matylda Mine) and B. pseudoaquensis sp. nov. from Ovčín locality are determined mainly on the basis of their spores, which are about 100 μm in diameter. The thick−walled exine of the miospores is laevigate or sometimes very finely scabrate on the proximal contact area. Spores resemble the dispersed species Punctatisporites obesus. Cones of B. brasensis and B. pseudoaquensis are organically connected with stems having prominent blade leaves and represent a new group of sphenophyllalean strobili.
According to a recent study, Ganoderma may be the third genus, after Alternaria and Cladosporium, whose spores cause symptoms of allergy and whose levels are directly related to meteorological factors. There are only few articles from different parts of the world about the relationships between Ganoderma spore count and meteorological factors. The aim of the study was to review all available publications about airborne Ganoderma spores and to compare the results in a short useful form.
Together with Alternaria, Cladosporium spores are known to be potent aeroallergens and its concentrations in the air are strongly dependent on meteorological factors. There are many articles from different parts of the world about relationships between Cladosporium spore count and weather parameters. The aim of the study was to review all available publications about Cladosporium spores in the air and compare the results in a short, useful form.
Aerobiological measurements were made by the volumetric method (VPPS Lanzoni and Burkard instruments). Concentrations of microscopic fungi were measured from April 16th to July 2nd, 2013, in two rooms of the Chair of Botany and Natural Environment Protection, Faculty of Biology, Szczecin University. The study was undertaken to perform mycological analysis of the air in selected rooms. Mycological contamination of the air in the surveyed areas was not diverse in terms of species composition. Nearly three times higher concentrations of fungal spores were recorded in the seminar room. The most abundant were spores of fungi from the genus Cladosporium. The concentration of fungal spores of Cladosporium, Botrytis and Aspergillus/Penicillium exceeds the limits.
Meriderma represents a recently described genus of nivicolous myxomycetes with high morphological variability. Due to many complications in its taxonomy and species recognition in the past, the group was considered a morphologically variable complex. Recent clarifications and recognition of morphological boundaries into species and morphotypes has fostered a classification revision of specimens found in the Carpathians. Material used in this study was systematically collected in the Polish part of the Carpathians from 2004 to 2009. As a result of micro- and macroscopic observations of 54 collections, we recorded nine taxa of Meriderma. Seven of these (all but M. carestiae and M. cribrarioides) are the first records for Poland and for the Carpathians overall. Our observations based on analysis of spore ornamentation by SEM are in accordance with recently proposed classification and confirm segregation of taxa based on spore ornamentation pattern.
The nested PCR has been used to evaluate the usefulness and efficiency of different Bacillus anthracis spore isolation methods in contaminated soil samples. The best results were obtained using two methods described by Beyer et al. [1] and Cheun et al. [9]. Outer and inner pairs of primers were designed from the protective antigen gene of plasmid pXO1 as well as from genes B and C of the capsule region of the plasmid pXO2. The influence of soil types on obtained results was also studied. The type of soil samples did not affect the nested PCR results. Furthermore, the sensitivity of nested PCR and PCR – ELISA was also examined.
The aim of the study was to investigate the effect of acidification and heat on the survival of Clostridium spores in third category animal by-products. Poultry minced meat and minced fish waste, contaminated with Clostridium sporogenes IW 1306 spore suspension, were subjected to the combined action of formic acid and high temperature of different values. To assess the efficiency of examined hygienization method, the spore number in biomass samples after acidification and heat treatment was estimated. For this purpose a 10-fold dilution series in peptone water was prepared and heat-treated at 80°C for 10 min. After cooling-down, one milliliter of each dilution was pour-plated onto DRCM medium solidified with agar. Statistical analyses were performed using the Statistica software. The results of the microbiological analysis show that lowering the pH to about 4 results in increasing the sensitivity of Clostridium sporogenes spores to a high – but not exceeding 100°C – temperature. The fastest inactivation of spores occurred after heating the acidified biomass at a temperature of 90°C. In the case of meat the 48-hour exposure to formic acid allowed the thermal inactivation of the spores within 15 minutes. The investigated method of hygienization may guarantee not only the inhibition of spores germination, but also enables their total elimination, reducing the epidemiological risk related to animal by-products handling.
The effect of nine fungicides and chitosan on the viability of Phomopsis viticola Sacc. spores occurring abundantly in pycnidia on carnation leaves, was tested in the laboratory conditions. The chemicals selected represent different mode of action on the pathogen and they were recognized as very effective in limiting P. viticola development within many viticultural regions. Fungicides were tested at six various concentrations of active ingredients, i.e. 10, 50, 100, 200, 500 mg. a.i./cm3 and at the concentrations that were recommended by the manufacturers. It was found that chlorothalonil, flusilazol and thiophanate – methyl caused the decay of all P. viticola spores in each studied concentration. After using mancozeb, cyprodinil, azoxystrobin and chitosan a strong inhibition of spore viability were also observed, especially at the concentrations of 100 mg of a.i. on cm3 and above. The other fungicides seem to be less effective compared with the above-metioned ones. Basing on the present studies and abundant information from literature, some of the most effective chemicals which should be tested on grapevine plantations in Poland were indicated.
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Experiments had been carried out on gametophytes and very young fronds of sporophytes with application of Murashige and Skoog (1962) medium. The paper described the results of 15 years in vitro experiments on 16 species of tree ferns belonging to various genera: Blechnum, Cibotium, Cyathea and Dicksonia. Genus Cyathea was represented by: C. australis (R.Br.) Domin., C. capensis (L.f.) Sm., C. cooperi (F.Muell.) Domin, C. brownii Domin, C. dealbata (G.Forest) Sw., C. dregei Kunze, C. leichhardtiana (F.Muell.) Copel., C. robertsiana (F.Muell.) Domin., C. schanschin Mart., C. smithii Hook.f. and Cyathea sp. In case of genus Dicksonia only two species were introduced into our experiments: D. fibrosa Colenso and D. sellowiana Hook.. Taxa Blechnum was presented by B. brasiliense Desv. and Cibotium by C. glaucum (Sm.) Hook. and Arn. and C. schiedei Schltdl. and Cham.. The studied species presented various responses on culture conditions depending on the level of stage of development. Time required for spores germination differed between species and took from only a few to 16 weeks. Prothalium formations showed various types of growth presented by marginal meristems. For all investigated species long term gametophyte in vitro cultures was established. Mature gametophyte possessed functional antheridia and archegonia. Spontaneous fertilization helped to establish the culture of young sporophytes. For all species the ex vitro culture in greenhouse collection was established. Manipulation of sucrose content in the medium stimulated the multiplication of gametophytes, but its lack induced formation of gemmae. Apospory was observed when culture of very young fronds was extended for 6 months and new generation of gametophytes was developed. Finally, sporophytes of 12 species were obtained and they have been growing in our greenhouse.
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