Wyniki wyszukiwania

1

Cytotoxicity of anticancer aziridinyl-substituted benzoquinones in primary mice splenocytes

100%

Miliukiene V., Nivinskas H., Cenas N.

Acta Biochimica Polonica

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2014

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tom 61

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nr 4

2

Thymocyte and splenocyte subpopulations in normal and leukemia - bearing mice after adrenalectomy

100%

Kawiak J., Hoser G., Malendowicz L. K., Miks B., Skurzak H.

Folia Histochemica et Cytobiologica

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1996

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tom 34

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nr 2

3

Inhibitory effect of antipsychotic drugs on the CON A- and LPS- induced proliferative activity of mouse splenocytes: a possible mechanism of action

84%

Basta-Kaim A., Budziszewska B., Jagla G., Nowak W., Kubera M., Lason W.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 2

Antipsychotic drugs are widely used to alleviate a number of psychic disorders and have been found to modulate some immune parameters, but the molecular mechanism of their action on the proliferative activity has been poorly recognized. In the present study, we investigated effects of various antipsychotics on the proliferative activity of lymphocytes stimulated by concanavalin A (Con A) and lipopolysaccharide (LPS). Chlorpromazine (3x10-6 - 10-4M) showed the most potent effect in inhibiting 3H-thymidine incorporation into C57BL/6 mouse spleen cells stimulated by Con A and LPS. Treatment of the cells with thioridazine (10-5 - 10-4M), promazine (10-5 - 10-4M), haloperidol (10-5 - 10-4M), risperidone (10-5 - 10-4M), raclopride (3x10-5 - 10-4M), remoxipride (3x10-5 - 10-4M) and clozapine ( 3x10-5 - 10-4M), but not with sulpiride (10-7 - 10-4M), suppressed proliferative activity of splenocytes after Con A stimulation. On the other hand, LPS-induced proliferation of splenocytes was inhibited by clozapine, promazine, thioridazine and haloperidol, but not by risperidone, remoxipride, sulpiride and raclopride. In the next part of the study, the influence of some kinase modulators on chlorpromazine- and clozapine-evoked inhibition of the proliferative activity of splenocytes was determined. Wortmannin, a selective phosphatidylinositol 3-kinase (PI3-K) inhibitor, blocked chlorpromazine and clozapine inhibitory effect on the mitogen-stimulated splenocyte proliferation. The involvement of PI 3-K /protein kinase B (PKB, Akt) pathway was confirmed by the results of the Western blot study, which showed that both drugs increased the level of active phospho-Ser-473 Akt, without changing the total Akt level, and decreased the level of active, nonphosphorylated glycogen synthase kinase-3 (GSK-3ß). Additionally, we have found that chlorpromazine action was also attenuated by a selective p-38-MAPK inhibitor, while clozapine effect was suppressed by a protein kinase C (PKC) activator. The obtained results indicated that atypical antipsychotic drugs markedly inhibited the proliferative activity of splenocytes only after ConA stimulation. Inhibition of the proliferative capability of splenocytes by chlorpromazine and clozapine resulted mainly from the activation of PI3-K/Akt pathway.

4

The effect of endotoxin administration on cytokine production in obstructive jaundiced rats

84%

Dawiskiba J., Zimecki M., Kwiatkowska D., Kuzminski A.

Archivum Immunologiae et Therapiae Experimentalis

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2001

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tom 49

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nr 5

5

In vitro IL-4, IL-12, and IFN-gamma production by splenocytes from Ancylostoma ceylanicum infected hamsters

67%

Dlugosz E., Wisniewski M., Wojcik L., Wedrychowicz H.

Bulletin of the Veterinary Institute in Puławy

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2010

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tom 54

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nr 3

The aim of the study was the production of chicken polyclonal antibodies recognising hamster IL-4, IL-12p35, and IFN-γ, which were further used to estimate cytokine concentrations in splenocyte culture supernatants, obtained from three age groups of Ancylostoma ceylanicum infected hamsters. Worm burdens differed significantly among age groups; the younger animals were the more heavily infected. Th1 and Th2 type cytokines were detected only in cultures containing spleen cells from the oldest hamsters, which were the most resistant to hookworm infection. In cultures of cells from young more susceptible hamsters, the presence of IL-4 and IL-12 but not IFN-γ were observed.

6

Immunotoxicity of airborne dust particles in young chickens of both sexes

67%

Bultowicz M. A., Buraczewska M., Markowska M., Skwarlo-Sonta K.

Acta Biologica Cracoviensia. Series Zoologia

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2001

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tom 43

7

Effect of aging on UVC-induced apoptosis of rat splenocytes

67%

Radziszewska E., Piwocka K., Bielak-Zmijewska A., Skierski J., Sikora E.

Acta Biochimica Polonica

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2000

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tom 47

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nr 2

UVC-induced apoptotic symptoms such as morphological changes, DNA fragmentation, Bcl-2 and Bax protein expression were examined in primary splenocyte cultures from young (3 months) and old (24 months) rats. The activities of AP-1 and CRE transcription factors in UVC-irradiated splenocytes were also assessed. At 24 h after UVC irradiation 40% of cells derived from young rats were found to be apoptotic, which was twice as much as in splenocytes from old rats. Apoptosis in cells from old rats did not give typical symptoms like a DNA ladder and Bcl-2 protein downregulation, in contrast to splenocytes from young rats. No AP-1 transcription factor activity was found in UVC-irradiated splenocytes from old animals and only a trace activity in splenocytes from young animals. This indicates that, UVC-induced apoptosis in rat splenocytes is practically AP-1 independent and that cells from old rats are less sensitive to UVC irradiation than splenocytes from young rats.

8

Fraction of spleen cells responsible for suppression of cellular immune response to SRBC in mice treated with outer membrane proteins of Shigella

67%

Czarny A., Witkowska D., Mulczyk M.

Archivum Immunologiae et Therapiae Experimentalis

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1992

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tom 40

|

nr 2

9

The effect of amphetamine sensitization on mouse immunoreactivity

67%

Kubera M., Filip M., Basta-Kaim A., Nowak E., Budziszewska B., Tetich M., Holan V., Korzeniak B., Przegalinski E.

Journal of Physiology and Pharmacology

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2002

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tom 53

|

nr 2

Recent studies indicate a role of the immune system in the behavioral effects of amphetamine in rodents. In the present study we attempted to find a connection between the behavioral changes induced by repeated, intermittent administration of amphetamine and some immunological consequences of sensitization to amphetamine in mice. Male Albino Swiss mice were treated repeatedly (for 5 days) with amphetamine (1 mg/kg, i.p.). On day 9, they received a challenge dose of amphetamine (1 mg/kg). Acute administration of amphetamine increased their locomotor activity by ca. 40%. In animals treated repeatedly with amphetamine, the challenge dose of the psychostimulant induced behavioral sensitization, i.e. the higher locomotor activation as compared with that after its first administration to mice. Immune functions were evaluated by the ability of splenocytes to proliferate and to produce cytokines such as interferongamma(IFN-gamma ), interleukin (IL)-4 and IL-10. Acute amphetamine administration significantly decreased, by ca. 30% and 25%, the proliferation of splenocytes in response to an optimal and a suboptimal dose of concanavalin A (Con A), respectively, and increased their ability to produce IL-4. Chronic intermittent treatment with amphetamine significantly decreased, by ca. 65% and 50%, the proliferative response of T cells to an optimal and a suboptimal dose of Con A, respectively, and diminished by 20% the metabolic activity of splenocytes. The above data showed that both acute and chronic amphetamine administration diminished some aspects of the cell-mediated immunity; nevertheless, immunosuppression was particularly evident in amphetamine-sensitized mice. Our findings seem to indicate possible importance of monitoring and correcting immune changes in the therapy of amphetamine addiction.

Ograniczanie wyników

Cytotoxicity of anticancer aziridinyl-substituted benzoquinones in primary mice splenocytes

Thymocyte and splenocyte subpopulations in normal and leukemia - bearing mice after adrenalectomy

Inhibitory effect of antipsychotic drugs on the CON A- and LPS- induced proliferative activity of mouse splenocytes: a possible mechanism of action

The effect of endotoxin administration on cytokine production in obstructive jaundiced rats

In vitro IL-4, IL-12, and IFN-gamma production by splenocytes from Ancylostoma ceylanicum infected hamsters

Immunotoxicity of airborne dust particles in young chickens of both sexes

Effect of aging on UVC-induced apoptosis of rat splenocytes

Fraction of spleen cells responsible for suppression of cellular immune response to SRBC in mice treated with outer membrane proteins of Shigella

The effect of amphetamine sensitization on mouse immunoreactivity