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Herbs and spices are commonly used in cuisine all over the world. They may contain many various substances beneficial for health, but also harmful (e.g. metallic) elements. Spices and herbal plants contain metal ions over a wide range of concentrations. Metals can have important positive or negative roles in human life. The aim of this study was to estimate the trace metals content in select popular Polish herbs and spices available on the Polish market. Thirty samples of various herbs and spices (fennel, sage, oak bark, St. John's wort, linden inflorescence, mint, black pepper, garlic, marjoram, paprika, cinnamon, basil, oregano, herbs de Provence, and parsley) were analyzed. The contents of Cu, Ni, Fe, Zn, and Mn were determined using AAS method after sample mineralization with 65% nitric acid and 30% hydrogen peroxide. Metals contents were compared in spices from different manufacturers. The chemometric techniques were used to evaluate similarity with respect to herb and spice metal contents.
Drying is the most common and also the simplest method of preserving fresh plant materials. This process greatly extends the life of the product by the removal of water, which decreases the rate of chemical and enzymatic reactions, or even inhibits them. The drying step can cause a change in appearance, taste, color and consistency, as well as reduce the quantity of essential oils, polyphenols, pigments and vitamins. These changes can be significantly reduced by using suitable drying techniques, depending on the material. The choice of the technique should be based on knowledge of the biological, physical and chemical characteristics of the raw material. The paper presents the effects of drying on the quantitative loss of essential oils and compounds with antioxidant properties, as well as changes in the antioxidant properties of selected herbs and spices dried by different methods. Both, the food industry and the cosmetics or pharmaceuticals industry generates a demand for high-quality raw materials, and hence research is being conducted both on preservation methods and on their impact on the quality of the obtained material. It seems that the new technology of drying material in a fluidized bed, greatly reduces or even eliminates the disadvantages of current procedures.
The present study was under taken in order to analyze the chemical mutagenesis on Chilli germplasm. In this regard, K1 variety of chilli was subjected to different mutagenic concentration for inducing mutagenesis. The M3 plants exposed to EMS and DES to produce clear difference from the untreated control, thus indicating that mutagenic treatment produce polymorphic regions in the chilli. For extraction of genomic DNA was adopted an improved protocol of CTAB method with slight modification. A total of ten primers were used to screen the polymorphism among the treated populations line tall, tall with chlorophyll deficient, leaf, flower, GMS and DNA damages in maturity mutants were analyzed with control. Out of ten primers, four primers (PGF02, PGF03, PGF04 AND OP107) were successfully amplified in all the samples used for this study. The successful primers were amplified in to 93 products showing an average of 9.3 bands.
The antimicrobial activity of some food additives used in meat products such as cumin, cinnamon, cloves, crushed red pepper, fennel, and anise against some microorganisms was investigated. For this purpose, the diethyl ether-treated extracts of spice samples were tested in vitro with Staphylococcus aureus ATCC 25923, Klebsiella pneumoniae FML 5, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 15753, Mycobacterium smegmatis CCM 2067, Micrococcus luteus A 2971, and Candida albicans ATCC 60192 as test strains. The disc diffusion method was applied in the trial. Cinnamon was found to be the most effective spice against tested microorganisms. The weakest antimicrobial activity was displayed by fennel. Crushed red pepper and anise were found to be ineffective against the test strains.
The composition of the hop extract obtained by extraction with liquid C02 under differ­ent conditions has been investigated. The highest yield of extract (9.7%) is obtained at temperature of 18-22°C, pressure of 54-58 atm. and time of 210 min. The extract contains 44.6% of a- and 19.3% of ß-acids and it can be used in brewing.
Thanks to the research it was possible to evaluate how useful is the automated TEMPO® system (bioMerieux) for determining the total number of mesophilic microorganisms in spices and food additives, characterised by a high concentration of dyes. Statistical analysis of the results by linear regression proves the equivalence of the TEMPO® method and the standard count plate method, at a correlation coefficient of 0.99. According to the findings, the dye of the examined matrices does not influence the result of the fluorescent signal at sample dilution of 1/4 000, which results in microbiological matrices contamination, not lower than 1.0 x 103 CFU/g. In the case of colourful food matrices demonstrating a high level of contamination with mesophilic microflora, such as whole black peppercorns (the level of microflora above 4.9 x 106 CFU/g), sample dilutions of 1/40 000 should be applied.
The composition of the extract from coriander fruits (Coriandrum sativum L.) obtained by extraction with liquid C02 has been investigated. The extract yield is 0.8%. Yield of vola­tile components and glyceride oil is 0.43% and 0.13% (with respect to the raw material), respectively and 55% and 16.5% (with respect to the extract), respectively. The main vola­tile components were linalool (71.6%) and a-pinene (6.3%). Many fatty acids of glyceride oil are petroselinic+oleic (C,8:1 66.9%) and palmitic (C16:0 8.6%).
Herbs and spices are sources of many bioactive compounds that can improve the taste of foods as well as influence digestion and metabolism processes. They can also contain some undesirable components that can be harmful, e.g. micotoxins, pesticides, heavy metals, and polycyclic carbohydrates residues. The objective of this study was to determine Pb, Cd, Zn, and Cu contents in the most popular spices and herbs used in Polish cuisine. The content of these metals was assayed by the AAS method. The results were compared with the safety standards established by the National Ministry of Health. Excessive amounts of Pb were found in 40% of basil, 42% of cinnamon, 25% of savory and 6% of dried onion samples. Increased levels of Cd were detected in 20% of basil, 25% of savory and 42% of cinnamon samples. Zn and Cu levels in all herbs and spices were within the safe limits.
The antifungal effect of twenty powdered spice plants and their extracts at concentrations of 2, 4,8 and 1, 3, 6%, respectively was evaluated in relation to the radial mycelial growth of various soilbome fungi causing damping-off disease. The spice powder or extract were added to the culture medium PDA to obtain the proposed concentrations. Concentration of 8% of powdered spices and 6% of their extracts were able to cause complete growth inhibition of major tested fungi. High significant inhibitory effect on radial fungal growth was observed for different concentrations of carnation (Dianthus caryophyllus), cinnamon (Cinnamomum burmannil), garlic (Allium sativum) and thyme (Thymus vulgaris). Meanwhile, fennel (Foeniculum vulgare), marjoram (Origanum majorana) and chamomile (Matricaria hamomilla) showed a low inhibitory effect on tested fungi. Moderate inhibitory effect was observed with the other tested spices. In the greenhouse, efficacy of spice plants as powder or their extracts in addition to the fungicide Rizolex-T used as seed dressings against faba bean damping-off incidence was evaluated in pot experiment using soil artificially infested with the disease agents (Fusarium solani and Rhizoctonia solani). Spice extracts showed superior reducing effect on damping-off disease incidence at pre-emergence growth stage to that of powder treatments and Rizolex-T as well, while an opposite effect was observed at post-emergence growth stage. Carnation and cinnamon spices showed the highest protecting effect against disease incidence when applied as powder or extracts. It is interesting to note that spice plants as powder or extracts gave a similar effect to the fungicide Rhizolex-T in reducing damping-off incidence either at pre- or post-emergence stages of faba bean growth. Promising applicable technique could be suggested in the light of the results obtained. The use of spice plants as powder or extract for seed dressing might be considered as safe, cheep and easily applied method for controlling soilbome plant pathogens considering the avoidance of environmental pollution and the side effect of pesticide application.
The carried out investigations evaluated ovicidal activity of mahlab, Prunus mahaleb L. kernel oil against cotton leafworm, Spodoptera littoralis (Boisd.). The chemical constituents of the fixed oil of mahlab were analyzed using gas-liquid chromatography (GLC). Timnodonic (33.07%), oleic (28.71%) and linoleic (24.35%) were the basic fatty acids, while the major hydrocarbon and sterol were found to be heneicosane (62.57%) and β-sitosterol (10.57%). The LC50 values for the one-day-old egg masses were found to be more susceptible than 3-day-old ones. Moreover, the leaf dip technique occurred to be more efficient than spraying technique. The results also showed abnormalities in the external morphology of egg shell, chorion surface, shell imprints and aeropyles of S. littoralis eggs treated with mahlab and KZ oils as compared to a control by using scanning electron microscope. Generally, the tested oils significantly reduced the activities of transaminase enzymes (AST and ALT), acid and alkaline phosphatases and total soluble protein except mahlab oil on acid phosphatase as compared to a control. Additionally, the oils of both mahlab and KZ oil affected some biological aspects such as incubation period, larval duration, larval mortality and pupal weight comparing to a control.
The purpose of the study was to determine antioxidant properties of acetone extracts from selected fresh and dried spices. Three spices have been examined: garlic, oregano and rosemary. Preparations were obtained by the extraction of polyphenols with a 70% acetone solution. The contents of total polyphenols and total catechin were determined in the study. The ability of total polyphenols contained in the obtained extracts to chelate Fe(II) ions was examined, too. The antioxidant properties of the examined extracts were assayed with three methods: against stable, synthetic DPPH radicals, cation-radicals ABTS and against hydroxyl radicals. On the basis of findings it turned out that condensed tannins are a dominant group of polyphenols in oregano, rosemary and fresh garlic. Granular garlic contained a higher level of catechins than tannins. All preparations of fresh spices dispalyed the ability to chelate iron ions. All extracts had antioxidant properties against synthetic radicals, with the exception of garlic preparation which did not inhibit hydroxyl radicals. Extracts of fresh spices demonstrated better antioxidant properties.
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