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Selected clinical features fail to predict inflammatory gene expressions for TNF‑alpha, TNFR1, NSMAF, Casp3 and IL‑8 in tendons of patients with rotator cuff tendinopathy

100%

Struzik S., Czarkowska‑Paczek B., Wyczalkowska‑Tomasik A., Maldyk P., Paczek L.

Archivum Immunologiae et Therapiae Experimentalis

2021

tom 69

nr 1

Effect of endurance training on the sphingomyelin-signalling pathway activity in the skeletal muscles of the rat

100%

Dobrzyn A., Zendzian-Piotrowska M., Gorski J.

Journal of Physiology and Pharmacology

2004

tom 55

nr 2

The sphingomyelin signalling pathway has been shown to function in different skeletal muscle types. The aim of the present study was to examine the effect of endurance training on the functioning of the pathway in the muscles. The experiments were carried out on two groups of male Wistar rats: sedentary and trained for six weeks. 24h after cessation of the training rats were anaesthetized and samples of the soleus, red and white section of the gastrocnemius were taken. The content and composition of sphingomyelin-fatty acids and ceramide - fatty acids was determined by means of gas-liquid chromatography. The content of sphingosine and sphinganine was determined by means of high-pressure liquid chromatography. The activity of neutral Mg++-dependent sphingomyelinase was determined spectophotometrically using trinitrophenylaminolauroyl-sphingomyelin as the substrate. It has been found that training reduces the total content of sphingomyelin- and ceramide-fatty acids, increases the content of sphinganine and does not affect the content of sphingosine in individual muscle types. The activity of the enzyme in the muscles is also elevated. It is concluded that training affects functioning of the sphingomyelin -signalling pathway in skeletal muscles. The reduction in the content of ceramide may contribute to elevation in glucose uptake in skeletal muscles observed after training.

Effects of pioglitazone and high-fat diet on ceramide metabolism in rat skeletal muscles

80%

Zendzian-Piotrowska M., Baranowski M., Zabielski P., Gorski J.

Journal of Physiology and Pharmacology. Supplement

2006

tom 57

nr 10

101-114

Ceramide is involved in the pathogenesis of insulin resistance in skeletal muscles of humans and rodents. However, there are conflicting reports in the literature on the effect of thiazolidinediones (a new class of insulin sensitizing drugs) on skeletal muscle ceramide content. Therefore, the aim of our study was to examine the effect of pioglitazone on the level of ceramide and its metabolites and on the activity of the key enzymes of ceramide metabolism in different skeletal muscle types of the rat. The experiments were carried out on rats fed either a standard chow or a high-fat diet for 21 days. Each group was divided into two subgroups: control and treated with pioglitazone for 14 days. High-fat diet increased the content of ceramide in the soleus and in the red section of the gastrocnemius, but not in the white section of the latter. The activity of neutral Mg2+-dependent sphingomyelinase and acid sphingomyelinase was simultaneously reduced in all examined muscles. Administration of pioglitazone decreased ceramide level in the soleus and in the red section of the gastrocnemius in rats fed either diet. This effect could not be attributed to decreased rate of ceramide formation from sphingomyelin or to its augmented deacylation to sphingosine. Pioglitazone treatment reduced the concentration of plasma free fatty acids in rats fed on either diet. Therefore, we conclude that the drug decreased the muscle content of ceramide by reducing its de novo synthesis. The results of our study indicate that reduction in ceramide level may be one of the mechanisms by which pioglitazone improves skeletal muscle insulin sensitivity.

Ograniczanie wyników

1 Archivum Immunologiae et Therapiae Experimentalis

1 Journal of Physiology and Pharmacology

1 Journal of Physiology and Pharmacology. Supplement

2 Gorski J.

2 Zendzian-Piotrowska M.

1 Baranowski M.

1 Czarkowska‑Paczek B.

1 Dobrzyn A.

1 Maldyk P.

1 Paczek L.

1 Struzik S.

1 Wyczalkowska‑Tomasik A.

1 Zabielski P.

1 2021

1 2006

1 2004

Wyniki wyszukiwania

Selected clinical features fail to predict inflammatory gene expressions for TNF‑alpha, TNFR1, NSMAF, Casp3 and IL‑8 in tendons of patients with rotator cuff tendinopathy

Effect of endurance training on the sphingomyelin-signalling pathway activity in the skeletal muscles of the rat

Effects of pioglitazone and high-fat diet on ceramide metabolism in rat skeletal muscles