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  1. 3 Folia Histochemica et Cytobiologica

  2. 1 Acta Biochimica Polonica

  3. 1 Advances in Agricultural Sciences

  4. 1 Animal Science Papers and Reports

  5. 1 Annals of Agricultural and Environmental Medicine

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Autorzy help

  1. 2 Derezinska E.

  2. 2 Dobrzynska M. M.

  3. 2 Ludwicki J. K.

  4. 2 Tyrkiel E. J.

  5. 1 Ak K.

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  1. 2 2017

  2. 2 2015

  3. 1 2010

  4. 3 2009

  5. 1 2006

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1

Evaluation of two prototype directional freezing methods and a 2ml flattened straw for cryopreservation of boar semen

100%
Puglisi R., Bornaghi V., Severgnini A., Vanni R., Montedoro M., Galli A.
Animal Science Papers and Reports
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2017
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tom 35
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nr 4
Based on previous assessments on stallions, 40 ejaculates of 20 Duroc boars were split and evenly frozen with a conventional vapour freezing method and two directional, drum and directional, prototype methods using commercial extenders and relative standard procedures. The directional prototype was provided with a double internal setup that allowed the positioning of experimental 2ml flat straws with 1 billion sperm (Flat) in a fixed support, or both classical 0.5 ml paillettes with 250 million spermatozoa and flats in a rotating drum designed so as to ensure a more uniform heat exchange. Preliminary tests for individuation of the most appropriate thawing rate showed beneficial effects (P≤0.05) of thawing the sperm at 50°C for 13 s when compared to 42°C for 20 s, in terms of total motility (42.8±8.4% and 35.6±6.8%, respectively). With regard to freezing/packaging methods, major improvements (P≤0.05) were shown for the drum method with paillettes for total motility (38.6±14.2%) assessed immediately after thawing, when compared with the conventional (29.4±13.3%) and the directional methods with flats (30.2±12.8%), and for total motility (P≤0.01) assessed following incubation for 120 min at 37°C after thawing (24.8±11.6%) with respect to the conventional method (15.6±10.9%). Despite the statistical non-significance of results, both the prototype freezing approaches using the experimental flat straw showed some improvements in functional parameters assessed by cytofluorometry when compared to the conventional method.
2

The influence of selected factors on the beef bulls sperm quality used for artificial insemination

100%
Kunowska-Slosarz M., Przysucha T., Golebiewski M., Slosarz J., Balcerek M.
Electronic Journal of Polish Agricultural Universities. Series Animal Husbandry
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2015
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tom 18
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nr 1
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Is concentration and motility of male gametes related to DNA damage measured by comet assay?

100%
Dobrzynska M. M., Tyrkiel E. J., Derezinska E., Ludwicki J. K.
Annals of Agricultural and Environmental Medicine
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2010
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tom 17
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nr 1
73-77
4

High levels of bulky DNA adducts in human sperm correlate with impaired fertility

100%
Horak S., Polanska J., Widlak P.
Acta Biochimica Polonica
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2003
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tom 50
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nr 1
Progressive decline in fertility and sperm quality has been reported over the last few decades, especially in industrialized nations. It has been proposed that exposure to factors that induce damage in DNA of spermatogenic cells may significantly contribute to impaired fertility. Here, the 32P-postlabelling method was used to analyze the levels of bulky DNA adducts in sperm cells in a group of 179 volunteers, either healthy subjects or patients with an impaired fertility. The levels of DNA adducts were 1.35-fold higher in the infertile group as compared to healthy individuals (P = 0.012). Similarly, a significant negative correlation between the levels of DNA adducts and measures of semen quality (sperm concentration and motility) has been observed (P < 0.001). In addition, the levels of bulky DNA adducts in sperm cells positively corre­lates with amounts of leukocytes in semen, which were significantly higher in semen of infertile subjects.
5

Effects of litter standardization on boar sperm quality and testes size

84%
Owsianny J., Czarnecki R., Wejksza D.
Advances in Agricultural Sciences
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1993
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tom 02
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nr 1
6

Female infertility - effect of perinatal xenoestrogen exposure on reproductive functions in animals and humans

84%
Gotz F., Dorner G.
Folia Histochemica et Cytobiologica. Supplement
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2001
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tom 39
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nr 1
7

Effect of low temperature thawing procedure and post-thaw cold shock on frozen bull semen

84%
Nur Z., Segirkaya H., Dogan I., Soylu M. K., Ak K., Ileri I. K.
Medycyna Weterynaryjna
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2005
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tom 61
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nr 09
8

KIR, LILRB and their ligands’ genes as potential biomarkers in recurrent implantation failure

67%
Nowak I., Wilczynska K., Wilczynski J. R., Malinowski A., Radwan P., Radwan M., Kusnierczyk P.
Archivum Immunologiae et Therapiae Experimentalis
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2017
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tom 65
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nr 5
9

FSHbeta gene mutation in a female with delayed puberty and hypogonadism: response to recombinant human FSH

67%
Kottler M. L., Richard N., Chabre O., Alain S., Young J.
Folia Histochemica et Cytobiologica
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2009
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tom 47
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nr 5
10

New insights about the evaluation of human sperm quality: the aromatase example

67%
Galeraud-Denis I., Travert C., De Vienne C., Said L., Saad A., Carreau S.
Folia Histochemica et Cytobiologica
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2009
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tom 47
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nr 5
11

Mechanisms of the harmful effects of bacterial semen infection on ejaculated human spermatozoa: potential inflammatory markers in semen

67%
Fraczek M., Kurpisz M.
Folia Histochemica et Cytobiologica
|
2015
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tom 53
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nr 3
12
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

The effects of di-n-butyl phthalate on the germ cells of laboratory mice

67%
Dobrzynska M. M., Tyrkiel E. J., Hernik A., Derezinska E., Goralczyk K., Ludwicki J. K.
Roczniki Państwowego Zakładu Higieny
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2009
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tom 60
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nr 4
Phthalate are found in the environmental samples due to their wide use in the industry as plasticizers. Di-n-butyl phthalate (DBP) is mainly used in nitrocellulose and polyvinyl acetate products as well as in personal-care products. This study was performed to investigate the influence of exposure to DBP on the quantity and quality (motility, morphology) and DNA damage (induction of micronuclei and DNA strand breaks) of male mice gametes. The estimation of DBP residues was also done. Eight weeks exposure to DBP (500 mg/kg bw and 2000 mg/kg bw) did not significantly affect testes and epididymes weights as well as sperm count. DBP clearly diminished sperm motility, enhanced frequency of abnormal sperm heads and not significantly increased DNA strand breaks in germ cells as well as frequency of micronuclei in spermatids. There were no bioacumulation of DBP in mice. Results suggest that DBP may affect the male mice germ cells.
13

Influence of heavy metals and 4-nonylphenol on reproductive function in fish

59%
Popek W., Dietrich G., Glogowski J., Demska-Zakes K., Drag-Kozak E., Sionkowski J., Luszczek-Trojan E., Epler P., Demianowicz W., Sarosiek B., Kowalski R., Jankun M., Zakes Z., Krol J., Czerniak S., Szczepkowski M.
Reproductive Biology
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2006
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tom 06
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nr Suppl.1
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