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Sperm epigenetic profile and risk of cancer

100%

Wdowiak A.

Journal of Pre-Clinical and Clinical Research

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2014

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tom 08

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nr 2

Introduction and objective. The integrity, stability and composition of sperm chromatin are of great importance in the fertilizing potential of male gametes and their capacity to support normal embryonic development. In this study, the author presents the current state of knowledge about the sperm epigenetic profile and risk of cancer. Abbreviated description of the state of knowledge. The obtaining of pregnancy and the state of health of the baby depends on the quality of the genetic material of both the female and the male. Health behaviours and environmental factors directly affect the quality of sperm, as well as the human egg cell and, consequently, on the reproductive capabilities, the course of pregnancy and the state of the newborn. There exist two thoroughly investigated epigenetic modifications: DNA methylation and histone modifications. The process of DNA methylation can be also a fundamental factor contributing to the development of cancer, where epigenotype undergoes significant modifications. When considering numerous DNA aberrations in the male gamete, the most commonly encountered is DNA fragmentation, particularly in infertile subjects. Surprisingly, an intracytoplasmatic sperm injection study of mice oocytes, using spermatozoa with a high DNA Fragmentation Index (DFI), revealed that a considerable percentage of adults born as a result of this method, showed a significant increase in the incidence of abnormal behavioural tests, malformations, cancer and signs of premature aging. Summary. The issue of assisted procreation raises the need to look for an appropriate treatment for males with sperm chromatin abnormalities. As a result, the fight against smoking addiction becomes the obvious necessity. Moreover, the reasonable solution nowadays seems to be supplementation with micronutrients and folic acid. It has been proved that the process of DNA fragmentation is a phenomenon that intensifies over time. Therefore, there should be a pursuance for, as close as possible, to the moment of ejaculation, application of semen to reproductive techniques. Finally, epigenetic changes are suspected of being one of the factors responsible for the deterioration of male sperm parameters observed in recent decades.

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Evaluation of spermiogram in domestic pigs

100%

Kondracki S., Wysokinska A., Banaszewska D., Wozniak E.

Reproductive Biology

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2006

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tom 06

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nr Suppl.2

3

The effect of breed on freezability of semen of fancy fowl

100%

Siudzinska A., Lukaszewicz E.

Animal Science Papers and Reports

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2008

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tom 26

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nr 4

A simple cryopreservation method described in 1995 by Tselutin et al. was used for freezing the semen of four fancy fowl breeds: White Crested Black Polish (WCBP), Greenleg Partridge (GP),Italian Partridge (IP) and Black Minorca (BP). The differences in quality (ejaculate volume,osmotic pressure, sperm concentration and morphology) of fresh semen between evaluated breeds were observed, as well as the differences in semen freezability. The freezing-thawing process caused significant (P≤0.01) decrease in percentage of live, normal spermatozoa, with coincident increase in percentage of dead spermatozoa and spermatozoa with acrosome defect. In relation to the fresh semen, the number of live, normal spermatozoa that survived cryopreservation procedure constituted 18.1% in WCBP, 25.1% in GP, 26.2% in IP and 33.6% in BM semen.

4

Self-association of Chaetopterus variopedatus sperm histone H1-like. Relevance of arginine content and possible physiological role

100%

Salvati D., Conforti S., Conte M., Matassa D. S., Fucci L., Piscopo M.

Acta Biochimica Polonica

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2008

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tom 55

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nr 4

701-706

Self-association of histones H1 from calf thymus and from sperm of the marine worm Chaetopterus variopedatus was studied on native and glutaraldehyde cross-linked molecules by PAGE and by salt-induced turbidity measurements. Multiple polymers were generated by native sperm histone H1-like after glutaraldehyde cross-linking while the same treatment on its lysine- or arginine-modified derivatives and on somatic histone H1 failed to induce polymerization. This result suggests the relevance of arginine content in the formation of histone H1-like polymers particularly because Chaetopterus variopedatus and calf thymus histones H1 have similar content of lysine but different K/R ratio (2 and 15, respectively). Salt-induced turbidity experiments confirmed the high tendency of sperm histone H1-like to form oligomers, particularly in the presence of phosphate ions. Native PAGE analysis in the presence of phosphate supported this hypothesis. The reported results suggest that phosphate ions connecting lysine and arginine side chain groups contribute to the interaction of sperm histone H1-like with DNA in chromatin and mplay a key role in organization and stabilization of the chromatin higher order structures.

5

Assessment of the morphometry of heads of normal sperm and sperm with the Dag defect in the semen of Duroc boars

100%

Banaszewska D., Andraszek K.

Journal of Veterinary Research

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2021

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tom 65

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nr 2

6

Movements of fish sperm flagella studied by high speed videomicroscopy coupled to computer assisted image analysis

100%

Cosson J., Billard R., Cibert C., Dreanno C., Linhart O., Suquet M.

Polskie Archiwum Hydrobiologii

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1997

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tom 44

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nr 1-2

7

Molecular targets for immunocontraception

100%

Stoyka B., Domagala A., Kurpisz M.

Archivum Immunologiae et Therapiae Experimentalis

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2003

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tom 51

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nr 1

8

Studies on sperm biological value in cases of men infertility

100%

Kotwicka M., Butowska W., Filipiak K., Skonieczna D., Warchol J. B.

Folia Morphologica

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1998

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tom 57

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nr 1

9

Evidence for aromatase localization in cytoplasmic droplet of human immature ejaculated spermatozoa

100%

Rago V., Bilinska B., Palma A., Ando S., Carpino A.

Folia Histochemica et Cytobiologica

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2003

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tom 41

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nr 1

10

Intracytoplasmic injection of cytometrically sorted bull sperm

100%

Skrzyszowska M., Bochenek M., Katska L., Smorag Z.

Roczniki Naukowe Zootechniki

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2000

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tom 27

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nr 2

11

Changes in the qualities of stallion semen after incubation with Streptococcus zooepidemicus

100%

Danek J., Wisniewski E., Krumrych W., Dabrowska J.

Bulletin of the Veterinary Institute in Puławy

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1996

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tom 40

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nr 2

The influence of ß-haemolytic Streptococcus organisms at concentrations of 100 x, 200 x, and 500 x 10³ cells/ml sperm incubated at 37°C for 60 min with stallion semen suspension on the motility, viability and morphology of the semen, and AspAT activity in seminal plasma was examined. S. zooepidemicus at a concentration of 500 x 10³ cells/ml exhibited a particularly unfavourable effect on semen. All parameters measured altered significantly after a 60 min incubation. The activity of AspAT also changed during a prolonged incubation of sperm with a suspension containing 500 x 10³ S. zooepidemicus/ml semen; after a 24 h incubation the activity of AspAT was enhanced by more than 38% in comparison to the initial value.

12

Variation in sperm formation patterns in jumping plant-lice (Hemiptera: Psylloidea): a light microscopic study

84%

Labina E. S., Maryanska-Nadachowska A., Burckhardt D., Kuznetsova V. G.

Folia Biologica

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2014

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tom 62

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nr 4

13

Oocyte-sperm interactions in the fertilization of birds

84%

Krawczyk A., Jaworska-Adamu J.

Medycyna Weterynaryjna

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2019

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tom 75

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nr 05

A thorough understanding of the mechanisms leading to the interaction between the sperm and the ovum in the process of fertilization in birds can facilitate more effective programming and control of the reproduction of these animals in breeding farms. In addition, it may allow the introduction of extracorporeal fertilization techniques, which may be important in the creation of transgenic animals and the reproduction of endangered species. In birds, the process of fertilization is not well known. It is conditioned by a series of interactions between mature reproductive cells. Oocytes are formed in the ovarian follicles of the left ovary. After ovulation, an ovum in the metaphase of the second meiotic division enters the oviduct along with the inner perivitelline layer (IPVL). It gets fertilized in this infundibulum. Male gametes are formed in paired testes located in the abdominal cavity. Sperm cells in the female reproductive tract do not require capacitation and are already fully capable of fertilization. As a result of internal insemination, male reproductive cells enter the oviduct. In this organ, they are selected and stored in the primary and secondary sperm storage tubules of the mucous membrane. They are released in batches shortly before ovulation. After reaching the oocyte, the sperm binds to the IPVL. This induces an acrosomal reaction that allows the male reproductive cells to penetrate to the surface of the oocyte, especially at the germinal pole. Next, as a result of physiological polyspermy, many sperm cells reach the ooplasm where they form haploid male pronucleus. This phenomenon is necessary to activate an polylecithal egg and produce a haploid female pronucleus. In the final stage, the female pronucleus merges with the single male pronucleus, which leads to the formation of a diploid zygote. The excess male pronuclei present in ooplasm are broken down by endonucleases (DNases). Understanding the mechanisms leading to the interaction between sperm and oocyte in birds may allow for more accurate programming and breeding of these animals in poultry farms and the introduction of extracorporeal fertilization techniques. In addition, it could be useful for the reproduction of endangered bird species.

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Boar variability affects sperm metabolism activity in liquid stored semen at 5 degrees Celsius

84%

Dziekonska A., Strzezek J.

Polish Journal of Veterinary Sciences

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2011

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tom 14

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nr 1

Metabolic activity of boar spermatozoa, liquid stored for three days at 5℃, was measured using bioluminescence for ATP content, fluorescent assay (JC fluorochrome) of mitochondrial activity and oxygen consumption. Sperm motility and plasma membrane integrity (PMI) were simultaneously analyzed. Apart from the statistically significant effect (P < 0.001) of semen storage time, the importance of the individual source of the ejaculate for the analyzed parameters of metabolic efficiency of spermatozoa was shown. This phenomenon was manifested in the interaction of the individual source of the ejaculate with spermatozoa motility, integrity of their membranes and metabolic activity with the passing time of semen preservation. Recorded results indicate that the individual factor may have a significant influence on the technological usefulness of boar spermatozoa for liquid storage. Quality analyses conducted on boar semen stored at 5℃ may be used for pre-selection of boars producing sperm with an enhanced tolerance to cold shock.

15

Respiration of bull spermatozoa in presence of exogenous glutathione (GSH)

84%

Slaweta R.

Acta Physiologica Polonica

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1987

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tom 38

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nr 1

16

Influence of selected extenders for liquid storage at 4 degrees Celsius of breeding chinchilla (Chinchilla lanigera) semen on sperm DNA integrity

84%

Niedbala P., Szeleszczuk O., Kuchta-Gladysz M., Joneczek M., Dobrzynska M., Maj D.

Folia Biologica

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2015

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tom 63

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nr 4

17

The effect of interaction between autosomal genes and genes on Y chromosome on sperm quality parameters in mice

84%

Marciniak M., Styrna J.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2008

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tom 50

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nr 1

18

Protective effects of L-carnitine on reproductive capacity in rats with diabetes

84%

Li X.-F., Shi Z.-D., Song H., Wang Y.-L., Li Q.-C., Diao X.-H., Pang Y.-W., Zhou S.-H., Liu H.-Y.

Journal of Physiology and Pharmacology

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2021

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tom 72

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nr 1

19

Effect of electromagnetic field on the sperm characteristics and histopathological status of testis in rats

84%

Aydin M., Turk G., Yuksel M., Cevik A., Apaydin A. M., Yilmaz S.

Medycyna Weterynaryjna

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2007

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tom 63

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nr 02

178-183

The aim of the study was to investigate the effects of electromagnetic fields ELF-EMFs generated by 170 kV (50 Hz) high power lines on the epididymal sperm characteristics, biochemical parameters testosterone levels and histopathology of testis. The experimental group consisted of 28 adult male rats placed in an cottage 7.5 m far from transfer lines transferring 170 kV (50 Hz) energy. They were randomly divided into four groups of 7 rats’ each. Group 1, 2 and 3 were exposed continuously (24 hr) to ELF-EMF (48.21 ± 1.58 mG) for 1, 2 and 3 months, respectively. The rats of group three served as the control and were placed in laboratory conditions without a magnetic field. Insignificant (p > 0.05) decreases were determined among the groups in terms of reproductive organ weights, testes dimensions and epididymal sperm concentration and sperm motility, and an insignificant increment was observed in abnormal sperm rates in relation to the varying periods of exposure to the ELF-EMF. Although marked reductions (p < 0.001) were observed among the groups in relation to plasma and testis catalase activity, depending on exposure time, no significant differences were found in terms of glutathione and malondialdehyde levels. In the light of Johnsen’s testicular biopsy score the mean scores of groups 1, 2, 3 and control were determined as 9.24 ± 0.08, 8.02 ± 0.12, 6.98 ± 0.11 and 9.88 ± 0.07, respectively. Histopathology examinations of testis revealed a deceleration of spermatogenesis and degeneration of germ cell order in relation to exposure time.

20

Effect of platelet activating factor (PAF) supplementation in semen extender on viability and ATP content of cryopreserved canine spermatozoa

84%

Kordan W., Lecewicz M., Strzezek R., Dziekonska A., Fraser L.

Polish Journal of Veterinary Sciences

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2010

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tom 13

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nr 4

The aim of this study was to investigate the effect of platelet activating factor (PAF) on the quality characteristics of cryopreserved canine spermatozoa. Cryopreserved semen of 5 mixed-breed dogs was treated with different concentrations of exogenous PAF (1 × 10⁻³M, 1 × 10⁻⁴M, 1 × 10⁻⁵M and 1 × 10-6M) and examined at different time intervals (0, 30, 60 and 120 min). Cryopreserved semen treated without PAF was used as the control. Sperm quality was evaluated for motility (computer-assisted semen analysis, CASA), mitochondrial function (JC-1/PI assay) and plasma membrane integrity (SYBR-14/PI assay and Hoechst 33258). Also, ATP content of spermatozoa was determined using a bioluminescence assay. Treatment of cryopreserved semen with 1 × 10⁻³ M PAF at 120 min of incubation resulted in significantly higher total sperm motility compared with the control. It was observed that PAF-improved total sperm motility was concurrent with enhanced sperm motility patterns after treatment of cryopreserved semen. Treatment of cryopreserved semen with PAF did not improve either sperm mitochondrial function or plasma membrane integrity, as monitored by different fluorescent membrane markers. Furthermore, ATP content of cryopreserved spermatozoa was significantly higher when PAF was used at a concentration of 1 × 10⁻³ M compared with the control and other PAF treatments, regardless of the incubation time. The findings of this study indicated that treatment with 1 × 10⁻³ M PAF at 120 min of incubation rendered better quality of cryopreserved canine semen, which was associated with improved sperm motility parameters and ATP content. It can be suggested that exogenous PAF addition is beneficial as a supplement for canine semen extender used for cryopreservation.

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