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EF-1alpha is a target site for inhibitory effect of quercetin in the peptide elongation process

100%

Marcinkiewicz C., Galasinski W., Gindzienski A.

Acta Biochimica Polonica

1995

tom 42

nr 3

The effect of quercetin (3,3',4',5,7-pentahydroxyflavone) on the polypeptide elongation system isolated from rat liver cells, was investigated. Quercetin inhibited [ 4C]leucine incorporation into proteins in vitro and the inhibitory effect is being directed towards the elongation factor eEF-1, but not to eEF-2 and ribosomes. Quercetin was found to form a complex with EF-la, which was inactive in GTP-dependent binding to ribosomes. It can be suggested that quercetin can block the total or the part of the domain of EF-la structure that is responsible for formation of the ternary complex EF-la-GTP-i14C]Phe-tRNA and therefore preventing formation of the quaternary complex with ribosomes.

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Effects of specific inhibitors on the gene expression of a digestive trypsin in Pieris brassicae L. (Lepidoptera: Pieridae)

63%

Sharifloo A., Zibaee A., Sendi J. J., Jahromi K. T.

Journal of Plant Protection Research

2018

tom 58

nr 1

Protein digestion in insects relies on several groups of proteases, among which trypsin plays a prominent role. In the current study, larvae of Pieris brassicae L. were fed radish leaves treated with 1 mM concentrations of three specific inhibitors of trypsin: AEBSF.HCl [4-(2- -aminoethyl)-benzenesulfonyl fluoride, monohydrochloride], TLCK (N-a-tosyl-l-lysine chloromethyl ketone) and SBTI (Soybean Trypsin Inhibitor) to find their potential effects on gene expression of trypsin. Initially, RT-PCR analysis revealed a gene of 748 bp responsible for synthesizing the digestive trypsin in P. brassicae larvae. Also, qRT-PCR data indicated a statistically greater expression of trypsin gene in the larvae fed 1 mM concentrations of AEBSF.HCl, TLCK and SBTI than the control. Results of the current study indicated that synthetic inhibitors can not only negatively affect the gene expression of P. brassicae trypsin, but also the insect can activate a compensatory mechanism against interruption of protein digestion by inducing more expression of the gene and producing more trypsin into the midgut lumen.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Journal of Plant Protection Research

1 Galasinski W.

1 Gindzienski A.

1 Jahromi K. T.

1 Marcinkiewicz C.

1 Sendi J. J.

1 Sharifloo A.

1 Zibaee A.

1 2018

1 1995

Wyniki wyszukiwania

EF-1alpha is a target site for inhibitory effect of quercetin in the peptide elongation process

Effects of specific inhibitors on the gene expression of a digestive trypsin in Pieris brassicae L. (Lepidoptera: Pieridae)