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The main aim of this study was to describe the variation between the populations of the dwarf mountain pine Pinus mugo Turra based on the morphological and anatomical traits of their needles, and to investigate the relationship between the observed variation and environmental conditions (altitude and substrate).Two-year-old needles were collected from 180 individuals of six populations of P. mugo growing in the Tatra Mts. Two populations were classified as dense, located at 1360–1450 m altitude, and the remaining four formed loose clusters and were situated at 1500–1650 m altitude. Four of the populations are growing on granite and two on a limestone substrate. The natural variation of 10 morpho-anatomical and 3 synthetic needle traits was measured. In addition to descriptive statistics, the analyses of variance (ANOVA) with a Tukey test and principal component analysis were computed. We also estimated Pearson correlation coefficients for the examined needle traits and altitude as well as substrate. Our results indicate that the P. mugo populations differ significantly with regard to the investigated traits for which the Trzydniowiański Wierch population was the most distinct. The observed pattern of variability is largely caused by differences in stomatal traits and these features are positive correlated with altitude. Additionally, populations growing on granite have larger values for most of the examined traits compared to populations growing on limestone.
Mountain pine (Pinus mugo) is the most important component of the sub−alpine and alpine zone in the Tatras, and other ranges of central Europe. In Poland, only population from the Karko− nosze Mts. has been studied in terms of phytopathological threats so far. There is not much information on the diseases caused by fungi in the mountain pine from other regions either. The mycological analyses of infected mountain pine needles were conducted for years 2013 and 2014 in subalpine zone of the Tatra Mts (Morskie Oko, Hala Gąsienicowa, Hala Kondratowa). Pine needles rust Coleosporium tussilaginis and yellow spots Lophodermium conigenum were found. To a lesser extent the needles were spotted with symptoms caused by Mycosphaerella dearnessi. Infection symptoms and fraction of the infected needles on one shoot were determined. The isolation of fungi was conducted in end of July, when the intensity of disease symptoms on needles was the highest. The main species isolated from the infected needles was Lophodermium spp., while the dominant one was L. conigenum. Except for this pathogen, L. pinastri, Botrytis cinerea and Epicoccum niger were identified together with several saprotrophic species, e.g. Aspergillus niger. The most frequent disease of mountain pine needles in the Tatra Mts. is pine needles rust. Observations in the Karokonosze and the Tatra Mts. indicate that both populations suffer from similar diseases. The differences show that pathogens adapt to different environmental conditions. Therefore constant monitoring of the health status of mountain pine growing in nature seems to be necessary.
Closely related Pinus species, mountain pine (Pinus mugo Turra) and Scots pine (Pinus sylvestris L.), belong to native woody species in the Tatra National Park (TPN, southern Poland). Their occurrence in close proximity can lead to the formation of natural hybrids known as Pinus × rhaetica, which is a woody, often polycormic form. Pinus×rhaetica is described in the TPN, but there has been a great deal of disagreement over its origin. The goal of the studies was to verify the taxonomic status of individuals identified as Pinus×rhaetica that grew in the eight stands together with P. mugo and P. sylvestris by SSAP (Sequence Specific Amplification Polymorphism) analysis of transposon insertional polymorphism. In total, 34 Pinus×rhaetica, 25 P. mugo and 27 P. sylvestris individuals were tested in addition to 20 individuals of P. uliginosa from ‘Torfowisko pod Węglińcem' and ‘Wielkie Torfowisko Batorowskie' as well as 25 individuals of P. uncinata from the Austrian Alps as the control groups. Four transposon sequences were employed: a DNA transposon from the CACTA family, Tpo and retrotransposons – two gypsy (Ogre, IFG7) and one copia like (Bare). All species belonging to the Pinus mugo complex are highly variable with 49−81% polymorphic loci and genetic diversity, HTequals 0.228−0.307 with the highest values in Pinus×rhaetica. Surprisingly, P. sylvestris proves to be the least variable species, likely because of a narrow gene pool in small, scattered stands in the Tatras. Very low Nei's genetic similarities between P. sylvestris and Pinus mugo complex, especially in comparison with P. uliginosa (I=0.548) and P. mugo (I=0.558) exclude unequivocally the possibility of spontaneous hybridization among these taxa. Thus, it undermines the hypothesis about hybrid origin of Pinus×rhaetica in the Tatras. It proves to be a morphological form of P. mugo as assessed from the Nei's coefficient, I=0.985 which is well within a range of conspecific populations. Finally, none of the studied individuals of Pinus×rhaetica are derived from seeds of Alpine P. uncinata.
The epiphytic, epixylic and terricolous lichens in the dwarf pine belt in the Babia Góra massif. Fragmenta Floristica et Geobotanica Polonica 11(2): 355–363. Kraków. PL ISSN 1640-629X. ABSTRACT: The paper contains a list of 69 lichens species, with their updated localities, which were found by the author in the dwarf pine belt in the Babia Góra Mt. in years: 1998–2001. Among the listed species, 20 are new to the Babia Góra Mt. The list contains 15 endangered species included in the “red list of endangered lichens in Poland”.
Mycological analyses of infected mountain pine needles were conducted for years 2011−2012. Yellow spots on needles were found in subalpine zone of the Karkonosze National Park. The isolation of fungi was conducted at the end of July in both years when the intensity of disease symptoms on needles was the highest. Infected needles were sampled from Kopa, Śląskie Kamienie, Kocioł Smogorni, Sokolnik, Łabski Szczyt, Szrenica, Kocioł Małego i Wielkiego Stawu study sites. The dominant species isolated from the infected needles was a probably new species of the genus Lophodermium. Except for this pathogen, Lophodermium pinastri, Botrytis cinerea and Epicoccum niger were identified together with several saprotrophic species, e.g. Aspergillus niger.
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