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1

An investigation of the date of sooty blotch primary infection and duration of incubation period for selected apple cultivars

100%
Grabowski M., Wrona B.
Folia Horticulturae
|
2004
|
tom 16
|
nr 1
73-77
2

The study of new fungus species causing apple sooty blotch

100%
Grabowski M.
Folia Horticulturae
|
2007
|
tom 19
|
nr 2
3

Influence of fructose and glucose occurring on fruit surface on the growth of fungi that cause sooty blotch of apple

100%
Wrona B., Grabowski M.
Journal of Plant Protection Research
|
2004
|
tom 44
|
nr 4
The fungi that cause sooty blotch grow only on the apple skin, so they use appropriate nutrients which are present on the fruit surface. It has been shown that when the first symptoms of sooty blotch occur a noticeable increase of glucose and fructose content both on the apple skin and in juice is observed. Such increase occurs at pH 4.4. An effect of surface glucose and fructose on the growth of pathogens responsible for the disease was also confirmed by evaluating the germination of conidia of Phialophora sessilis de Hoog and Peltaster fructicola Jonhson in solution of above mentioned sugars, distilled water and standard d-glucose solutions.
4

Etiology of apple sooty blotch in Poland

100%
Wrona B., Grabowski M.
Journal of Plant Protection Research
|
2004
|
tom 44
|
nr 4
Investigations have been undertaken to determine which fungi species are responsible for occurrence of sooty blotch disease in Poland. It was found that disease complex is caused by Tripospermum myrti (Lind) Hughes, Phialophora sessilis de Hoog and Peltaster fructicola Jonhson. There was no evidence of the presence fungus described as Gloedes pomigena which was previosly considered as a casual agent of apple sooty blotch disease in Poland.
5

Effect of surface amino acids on the growth of Peltaster fructicola - fungus associated with sooty blotch complex

80%
Wrona B., Gleason M.
Journal of Plant Protection Research
|
2005
|
tom 45
|
nr 4
273-278
Studies undertaken in 2002–2004 on ‘Golden Delicious’ apple fruits showed the presence of amino acids on the surface of their skin. Amount of total free amino acids ranged from 2.5 to 3.0 mg/L. In in vitro bioassays it was found that amino acids as a basic source of nitrogen did not activate germination of conidia of Peltaster fructicola (Johnson). However amino acids stimulated elongation of germ tubes and early hyphal growth. It was confirmed that fruit washings also contained sugars that stimulated both conidial germination and germ tube elongation. We proved also that different carbon to nitrogen proportions significantly affected P. fructicola growth. Proportions of C:N were evaluated according to newly introduced amino acid sugar index (ASI).
6
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Characteristics of Aureobasidium pullulans (de Bary et Lowenthal) G. Arnaud isolated from apples and pears with symptoms of sooty blotch in Poland

80%
Mirzwa-Mroz E., Winska-Krysiak M., Dzieciol R., Miekus A.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2014
|
tom 13
|
nr 3
Sooty blotch is a disease of apple and pear caused by a complex of fungi that blemish the fruit surface. Results of molecular studies indicated approximately 30 different fungi species associated with this disease. Apples and pears with symptoms of sooty blotch were collected in summer and early autumn 2006–2010 from trees grown in fungicide non-treated orchards and small gardens located in various regions of Poland. Fungi causing sooty blotch were isolated from fruits and the isolates were divided into six groups, according to their morphological characters. Growth of the fungi colonies were tested on different agar media (PDA, CMA, MEA and Czapek). The ITS region of rDNA from 16 isolates from the first group was amplified by PCR technique and one representative sequence of this isolates was used to alignment in Gene Bank. This isolate was identified as Aureobasidium pullulans and isolates from this group were compared with it on the base of morphological features.
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