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1

Optimization of growth conditions for xylanase production by Aspergillus niger in solid state fermentation

100%
Kavya V., Padmavathi T.
Polish Journal of Microbiology
|
2009
|
tom 58
|
nr 2
125-130
The objectives of the present study were isolation, identification and characterization of xylanase producing fungi, optimization of medium composition and cultural conditions for xylanase enzyme production using cheaper sources. The fungal strains were isolated from garden soil by serial dilution technique and Aspergillus niger was identified and isolated in pure form. In conformation screening by congo red test, based on the reddish zone of enzyme activity formation in oat spelt xylan agar plates, A. niger was selected and optimized for xylanase enzyme production in solid state fermentation using cheaper sources like wheat bran, rice bran, soya bran, ragi bran and saw dust. Maximum enzyme activity was observed in wheat bran (9.87 U/ml). The use of wheat bran as a major carbon source is particularly valuable because oat spelt xylan or birch wood xylan are more expensive. The effects of time course, incubation substrate, inoculum size, moisturizing agent, moisture content, temperature and volume of fermentation medium on the production of xylanase were studied. The maximum xylanase production (12.65 U/ml) was observed at optimized condition, incubation temperature of 28°C after 6 days of incubation period while minimum production (9.38 U/ml) at unoptimized condition. The maximum production of enzyme was found to be in wheat bran when the volume of fermentation medium was kept as 10 g/250 ml conical flasks, with mineral solution as moisturizing agent and moisture ratio 1:0.7. Thus the present study proved that the fungal strain A. niger used is highly potential and useful for xylanase production.
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Production and applications of xylanases – an overview

86%
Malhotra G., Chapadgaonkar S. S.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2018
|
tom 99
|
nr 1
3

The influence of heat and mass transfer on solid state fermentation

86%
Szewczyk K. W.
Acta Biochimica Polonica
|
1993
|
tom 40
|
nr 1
4
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Content available

Improving the monacolin K to citrinin production ratio in red yeast rice by an X-ray-induced mutant strain of Monascus purpureus

72%
Kanpiengjai A., Mahawan R., Pengnoi P., Lumyong S., Khanongnuch C.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2018
|
tom 99
|
nr 2
5
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Content available

Comparative studies on the production of statins using different microbial strains

72%
Syed M. B.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2019
|
tom 100
|
nr 3
6

Production and characterization of alpha-amylase from Aspergillus niger JGI 24 isolated in Bangalore

72%
Varalakshmi K. N., Kumudini B. S., Nandini B. N., Solomon J., Suhas R., Mahesh B., Kavitha A. P.
Polish Journal of Microbiology
|
2009
|
tom 58
|
nr 1
29-36
Five fungal isolates were screened for the production of α-amylase using both solid-state and submerged fermentations. The best amylase producer among them, Aspergillus niger JGI 24, was selected for enzyme production by solid-state fermentation (SSF) on wheat bran. Different carbon and nitrogen supplements were used to enhance enzyme production and maximum amount of enzyme was obtained when SSF was carried out with soluble starch and beef extract (1 % each) as supplements. Further attempts to enhance enzyme production by UV induced mutagenesis were carried out. Survival rate decreased with increase in duration of UV exposure. Partial purification of the enzyme using ammonium sulphate fractionation resulted in 1.49 fold increase in the enzyme activity. The enzyme showed a molecular weight of 43 kDa by SDS-PAGE. Metal ions Ca²⁺ and Co²⁺ increased the enzyme activity. The enzyme was optimally active at 30°C and pH 9.5.
7

Aspergillus-fermented Jatropha curcas seed cake: proximate composition and effects on biochemical indices in Wistar rats

72%
Faoziyat S. A., Amina A. E.-I. M., Adeyemo A. A., Muhammed R. B., Sulaiman A. M., Aliyu A. O., Adeyemi O. S.
Biological Letters
|
2014
|
tom 51
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nr 1
8

Physiological properties and enzymatic activities of Rhizopus oligosporus in solid state fermentations

72%
Miszkiewicz H., Bizukojc M., Rozwandowicz A., Bielecki S.
Electronic Journal of Polish Agricultural Universities. Series: Biotechnology
|
2004
|
tom 07
|
nr 1
9

Extraction of milk-clotting enzyme produced by solid state fermentation of Aspergillus oryzae

72%
Shata H. M. A.
Polish Journal of Microbiology
|
2005
|
tom 54
|
nr 3
Studies on the extraction of milk-clotting enzyme after solid-state fermentation (SSF) of wheat bran by a local strain of Aspergillus oryzae LSI were done. The extraction of the enzyme was found to be depended on different parameters like nature of extractant, soaking time, temperature etc. From different inorganic and organic extractants, calcium chloride (0.05%) and glycerol (40%) were found to be the best solvents for leaching out milk-clotting enzyme. The optimum volume of calcium chloride was 5 ml/g biomass. An extraction time of 180 min. at 30°C and 100 rpm was sufficient to extract out nearly 28% of the enzyme (2666.7 U/g biomass). Most of the enzyme (about 98.71%) was recovered in four repeated extractions.
10

Production of cellulases during citric acid biosynthesis by solid-state fermentation

72%
Gasiorek E., Lesniak W.
Polish Journal of Food and Nutrition Sciences
|
2002
|
tom 11/52
|
nr 1
The studies on optimisation of citric acid biosynthesis by solid-state fermentation of sugar-beet pulp as the major material have shown that not only mono- and disaccharides initially present in the material but also the products of cellulose and hemicellulose hydrolyses were the carbon sources. Therefore, in parallel with optimisation of citric acid production, the studies were undertaken on the activity of ccllulases and dynamics of their synthesis by Aspergillus niger, the strain used in the process. The fermentations were carried out by non-mixed system using 1 L beakers and by mixed system using 4.5 L rotating drum bioreactor. The activity of CMC-ase and xylanases for the non-mixed system increased to about 22.0 IU/g and that of FP-ase stayed on the level of about 4 IU/g dry matter, whereas for the mixed system the CMC-ase activity increased to about 35 IU/g dry matter on day five and that of FP-ase stayed on a similar level of about 5 IU/g dry matter. The content of reducing compounds increased by almost four times (to 390 g/kg dry matter) on the second day of fermentation and decreased to about 140 g/kg dry matter on the fifth day of fermentation. Glucose was utilised most rapidly among monosaccharides, being followed by arabinose and in part by mannose.
11

Extracellular xylanase production by Fusarium species in solid state fermentation

58%
Arabi M. I. E., Bakri Y., Jawhar M.
Polish Journal of Microbiology
|
2011
|
tom 60
|
nr 3
Fusarium sp. has been shown to be a promising organism for enhanced production of xylanases. In the present study, xylanase production by 21 Fusarium sp. isolates (8 Fusarium culmorum, 4 Fusarium solani, 6 Fusarium verticillioides and 3 Fusarium equiseti) was evaluated under solid state fermentation (SSF). The fungal isolate Fusarium solani SYRN7 was the best xylanase producer among the tested isolates. The effects of some agriculture wastes (like wheat straw, wheat bran, beet pulp and cotton seed cake) and incubation period on xylanase production by F.solani were optimized. High xylanase production (1465.8 U/g) was observed in wheat bran after 96 h of incubation. Optimum pH and temperature for xylanase activity were found to be 5 and 50°C, respectively.
12

Alpha-amylase production by Streptomyces erumpens MTCC 7317 in solid state fermentation using response surface methodology [RSM]

58%
Kar S., Ray R. C., Mohapatra U. B.
Polish Journal of Microbiology
|
2008
|
tom 57
|
nr 4
ProductiomnftFamylase under solid state fermentation by Streptomyces erumpens MTCC 7317 has been investigated using different agro-industrial residues, i.e. cassava bagasse, sugarcane bagasse and wheat bran; wheat bran was found to be the best substrate. Among different nitrogen source supplemented to wheat bran, beef extract or peptone (1 %) showed maximum enzyme production. Response surface methodology was used to evaluate the effect of main process parameters as incubation period (48 h), moisture holding capacity (70%), pH (7.0) and temperature (50°C) on enzyme production by applying a full factorial central composite design. The maximum hydrolysis of soluble starch (90%) and cassava starch (75%) was obtained with the application of 4 ml (~12096 U) of S. erumpens crude enzyme after 5 h of incubation.
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