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In this study, the biocontrol abilities of water-soluble and volatile metabolites of three different isolates of Trichoderma (T. asperellum,T. harzianum and Trichoderma spp.) against soil borne plant pathogen Rhizoctonia solani were investigated both in vitro and in vivo. The results showed for the first time that mycelial growth inhibition of the pathogen was 74.4–67.8% with water-soluble metabolites as compared to 15.3–10.6% with volatile metabolites in vitro. In vivo antagonistic activity of Trichoderma isolates against R. solani was evaluated on bean plants under laboratory and greenhouse conditions. We observed that T. asperellum was more effective and consistent, lowering disease incidence up to 19.3% in laboratory and 30.5% in green house conditions. These results showed that three isolates of Trichoderma could be used as effective biocontrol agents against R. solani.
Phytophthora plurivora was the most often detected species from water using rhododendron baits. The species was isolated from water of two rivers, Jasieniec and Korabiewka, a water pond and a drainage canal from March to November, 2008 (in Korabiewka river also in December). The highest population density of P. plurivora was observed in March and April in water pond and canal, and in May in both analysed rivers. In laboratory trials all tested isolates colonized rhododendron and poplar leaves. Isolates from drainage canal were the most pathogenic for rhododendron. Isolates detected in March from water pond and two rivers caused the quickest spread of necrosis on leaf blades. On poplar leaves the fastest development of necrotic spots was observed when isolates obtained in June and November were used for inoculation, while the isolate from September sample was less pathogenic.
Bioproduct on the basis of aurine applied in vitro decreased the growth and spore germination of 10 fungal species by 25 to 99%. Application of Trichodermin biopreparate in the control of cucumber Fusarium root rot significantly decreased the disease development.
Genotypic differentiation among 10 isolates of Phytophthora cinnamomi Rands and 24 isolates of Phytophthora citricola Sawada from 12 different plant species grown in Polish ornamental nurseries was determined. DNA was extracted from pure pathogen cultures and amplified by the PCR technique using ISSR and RAPD primers. 9 primers were used to amplify P. cinnamomi and 8 to amplify P. citricola DNA. The analyzed amplification products were between 300 and 2300 bp. The genotypical differentiation was from 17 to 35% in P. cinnamomi and from 10 to 60% in P. citricola. Isolates from host plants of the same family showed, with some exceptions, similar levels of differentiation.
Fusarium oxysporum and Rhizoctonia solani are the major soil-borne pathogens causing growth and yield depression. The present study focused on the ability of fluorescent Pseudomonas and Glomus fasciculatum on growth performance of Vigna radiata in pathogen-infested soil. The percent colonization by G. fasciculatum indicated an increase of the presence of fluorescent Pseudomonas and a decrease of the presence of Fusarium oxysporum or Rhizoctonia solani. However, the reduction of colonization induced pathogen in percent was alleviated by fluorescent Pseudomonas. Inoculation with either fluorescent Pseudomonas or G. fasciculatum or both induced a significant increase in root and shoot length, plant vigour index, dry weight and total N and P content in V. radiata as compared to uninoculated control. The impact of inoculation was much pronounced in dual inoculated plants in comparison with those inoculated with either G. fasciculatum or fluorescent Pseudomonas. In contrast, treatment of plants with either F. oxysporum or R. solani decreased the root and shoot length, plant vigour index, dry weight and total N and P content in the test legume. However, in the presence of fluorescent Pseudomonas and G. fasciculatum, the adverse effect on the pathogens on growth of V. radiata was alleviated.
The efficacy of applying biocontrol agents, chemical fungicide and nematicide as protective treatments against the soilborne parasites, Fusarium spp. (Fusaria) and citrus nematode Tylenchulus semipenetrans Cobb was evaluated. The experiment took place under field conditions in a citrus orchard cultivated with 16-year-old sweet orange (Citrus sinensis L.) osbech cv. Valencia trees grafted on sour orange (C. aurantium L.) rootstock during the growing season November 2006/ October 2007. This orchard is located at Bader district, Behera governorate, Egypt. The populations of soil fauna and flora under trees canopy were examined just before treatment, and 1, 3, 6, 9 and 12 months after the treatment application. A visual inspection for the appearance of symptoms related to Fusarium or nematode infection on treated and untreated citrus trees was carried out periodically every two weeks throughout the experimental period. The populations of Fusarium spp. were gradually decreased throughout the experimental period. However, the antagonistic bacterial isolates showed drastic effect for reducing the Fusaria population from 38.5% before treatments to a range of 1.2–4.0% after one month of such a bacterial application followed by the Kocide (fungicide) treatment which recorded 6.6%. Meanwhile, Fusaria populations of 18.4 and 16.3% were recorded as Fusaria population in treatments of the nematicide Carbofuran and entompathogenic nematode, respectively. Also, the population density of T. semipenetrans juveniles drastically decreased soon after all the treatment applications. Then after the nematode population build up during the growing season followed a natural distribution decline shape starting from the third month of application up to the ninth month, then it decreased. The citrus nematode increased steadily in the untreated check till September 2007 then its population level decreased. Treatments of Bacillus subtilis – B (20 ml) and Pseudomonas fluorescens (20 ml) gave the highest citrus yield followed by B. subtilis A (10 ml); B. subtilis B (10 ml) and Kocide (fungicide). Moreover, citrus trees treated with B. subtilis A (10 ml); P. fluorescens (10 ml) and Carbofuran (nematicide) had a higher yield production than trees treated with entomopathogenic nematodes Heterorhabditis egyptii (Abd-Elgawad and Ameen 2005). Yet, visual monitoring for disease incidence throughout the citrus orchard during the whole period of the study revealed no disease symptoms of any fusaria or nematode infection in treated trees. Untreated trees had a 1.9 and 3.1% fusaria and nematode infection, respectively. The importance of the present work, therefore, is based on the proposed bioagents as protective applications that are able to inhibit the citrus pathogens and prevent them from causing citrus damage.
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