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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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The optimal TTC dose and its chemical reduction level during soil dehydrogenase activity assay

100%
Wolinska A., Zapasek M., Stepniewska Z.
Acta Agrophysica
|
2016
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tom 23
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nr 2
2,3,5-triphenyltetrazolium chloride (TTC) is a dye largely used for determination of microbiological activity in soil samples by dehydrogenase assay. TTC is colourless in the oxidised form and red when reduced, due to formation of the product 1,3,5-triphenyl formazan (TPF). In this study, different doses of TTC ranging from 0.2 to 3.0% were added to six different soil types with the aim to verify the effect of chemical TTC reduction and to optimise the substrate dose in order to prevent a toxic effect on microorganisms. Regarding the technical simplicity and cost-effectiveness, the TTC assay is commonly recommended as an efficient method for determination of soil biological activity, provided that an appropriate concentration is applied, depending on the soil type. The lowest TTC levels of 0.2 and 0.5% are recommended for Albic Luvisol and Eutric Fluvisol, respectively, and the highest (2%) for Brunic Arenosol and Haplic Luvisol soils. It has also been demonstrated that DHA has biological origin in more than 90%, whilst chemical TTC reduction accounts for less than 10%, and therefore does not interfere with the correctness of the results obtained.
2

Response of poplar roots to heavy metal pollution: ‘vitality’ and secondary metabolism

84%
Michalak A., Lorenc-Plucinska G., Stobrawa K.
Acta Physiologiae Plantarum
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2007
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tom 29
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nr 1 Suppl.
3

Spatial variability of soil dehydrogenases and cellulases activities in a field scale

67%
Smolinski S., Dlugosz J., Piotrowska A., Zamorski R.
Polish Journal of Soil Science
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2008
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tom 41
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nr 1
73-80
Spatial field-scale variability of soil dehydrogenases and cellulases activities and their relationship with organic carbon and total nitrogen content at the surface horizon of eutric, gleic Cambisols of the field, under winter wheat localised in the southern part of the Sępopolska Plain was assessed. Twenty soil samples were collected in September in the system defined by the method of diffused points localised by GPS. The activity of dehydrogenases ranged between 0.8-1.55 µM TPF g⁻¹ DM 24 h⁻¹, while cellulolytic activity ranged between 0.2-1.0 µM glucose g⁻¹ DM h⁻¹ x 10⁻³. A high nugget effect observed for both enzymatic activities was probably caused by a nest (local) occurrence of the enzymes, especially dehydrogenase. No statistically significant correlation coefficients were calculated for the relationship between the enzymatic activities and Corg, Ntot and clay fraction content.
4
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Links between root carbohydrates and seasonal pattern of soil microbial activity of diverse European populations of Pinus sylvestris grown in a provenance plantation

59%
Kieliszewska-Rokicka B., Oleksyn J., Zytkowiak R., Reich P. B.
Acta Societatis Botanicorum Poloniae
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2003
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tom 72
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nr 2
Activity of soil dehydrogenase (DHA) was measured in the mineral soil in a forest stand of 15 to 16-year-old Scots pine (Pinus sylvestris L.) from geographically diverse populations, as an indicator of biological activity of soil microorganisms, in a provenance experiment in Poland. The pine populations originated from six European countries (Sweden, Russia, Latvia, Poland, Germany, France) and differed widely in aboveground biomass and productivity. Soil DHA during two growing seasons showed pronounced seasonal variability, which was significantly related to the fine root concentration of nonstructural carbohydrates. Higher DHA was found in soil under canopies of the central and southern European populations than in those from more northern parts of the Scots pine range. Significant positive correlation between soil DHA and aboveground tree biomass suggest that these patterns most likely resulted from differences in carbon dynamics and productivity among populations.
5

Changes of dehydrogenase inhibition in soil induced by various concentrations of selected heavy metal salts

59%
Nowak J., Snieg B.
Electronic Journal of Polish Agricultural Universities. Series Environmental Development
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2002
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tom 05
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nr 2
The influence of various concentrations of salts of lead (II), copper (II), manganese (II), cobalt (II), molybdenum (IV) and iron (III) upon the soil dehydrogenase has been studied. Ecological areas featuring the contamination degree to which the studied soil environment had been exposed have been determined. Particular attention has been paid to high heavy metal doses causing significant disturbance to soil metabolism.
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