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The purpose of presented study was determination of the impact of selected physicochemical parameters, strongly influencing the soil biological life: water potential (pF), oxygen availability for microorganisms (ODR), redox potential (Eh), content of Mg, Ca and total organic carbon (TOC) on soil DNA concentration. Undisturbed loess soil material was taken to metal cylin-ders (100 cm3) from four depths (0-20; 20-40; 40-60 and 60-80 cm), what make possibility for description of DNA content and its distribution in the whole soil profile. Our results revealed signif-icant (p<0.05) positive relationships between soil DNA content and measured values of ODR (r = 0.94***), Eh (r = 0.52**) and TOC (r = 0.98***), what were confirmed by high values of correla-tion coefficients (r). Whereas, significant negative interrelationships between soil DNA and pF (r = –0.57*) or Mg content (r = –0.79***) were determined. However, in the current experiment condi-tions, we did not found significant correlation between Ca presence and DNA content (p>0.05). Significant (p<0.05) decrease of DNA concentration by 62.8% with an increase of soil depth was noted, what was undoubtedly connected with spatial distribution of microorganisms in the soil profile and its likes for surface layers colonization.
The field experiment was carried out between 1994-1998 at the Agricultural Experimental Station of the Academy of Podlasie, Siedlce on the influence of the means of inoculation with Rhizobium galegae and Nostoc of the Galega orientalis (Lam.) seed variety Gale on the content of dry matter, nitrogen, total protein, ash and crude fiber as well as on the yield of this plant. The climatic conditions during the experiment (1994-1998) were not suitable (very low rainfall) for growing this plant which significantly decreased the yield of the dry mass. The highest yield (10.8 t ha-1) was harvested from the object in which the seeds were inoculated by Rhizobium galegae and Nostoc. Plants harvested from this object also had the highest content of nitrogen (30 g kg-1 d.m.) and total protein (187.5 g kg-1 d.m.). The low content of ash 59 g kg-1 d.m. high in crude fiber (385 g . kg-1 d.m.) were caused by the later than normal harvesting of Galega orientalis.
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