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Steroid hormones may act through a rapid mechanism that does not require an intracellular steroid receptor and its effects on gene expression. In this study we have analysed this so-called non-genomic effect of testosterone on social anxiety in rats of both sexes using androgen and oestrogen receptor blockers. Male rats were divided into four groups: SHAM-CTRL (a sham operated group treated with oil as vehicle, n=10), SHAM-TST (a sham operated group treated with testosterone at a dose of 1 mg/kg, n=10), GDX-CTRL (a castrated group treated with oil, n=10) and GDX-TST (a castrated group treated with testosterone at a dose of 1 mg/kg, n=10). Female rats were divided into two groups: OVX-CTRL (an ovariectomized group treated with oil, n=10) and OVX-TST (an ovariectomized group treated with testosterone, n=10). The intracellular androgen receptor was blocked with flutamide and both intracellular oestrogen receptors were blocked with tamoxifen (a selective oestrogen receptor modulator). Rats were tested one hour after oil or testosterone administration in the social interaction test. Although the concentration of testosterone was higher in testosterone groups, no significant difference in social interaction was observed between the groups. In summary, in this first study focusing on the non-genomic effects of testosterone on social interaction no rapid effects of testosterone in adult rats were found. Further studies should analyse potential nongenomic effects of testosterone on other forms of social behaviour.
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Sense of social interaction in the elderly

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The study of social behaviour can give us important insights into the social and mating system of a species or population. We investigated male–female interactions in captive bushveld gerbils, Gerbilliscus leucogaster, to gain insight into the social behaviour and mating structure. We conducted two experiments. In experiment 1, we studied the interactions between two strangers for a week in a three-tank setup, which tested behavioural variation spatially and temporally. Although tolerance between strange males and females increased with time, it remained generally low throughout the experiment. Females appeared to be cautious of males initially, but they later became aggressive towards them. Males showed an increase in submissive behaviour over time. The ‘home’ cage did not appear to be defended by either sex. In experiment 2, we investigated the social interactions of male–female pairs during pregnancy and lactation. Aggression persisted throughout the study and amicable behaviour was low; females were much more aggressive than males. We did not detect changes in social behaviour with the progression of pregnancy and lactation. We suggest that pair bonding is unlikely and that promiscuity is the most probable mating system. Female aggression may be related to mate choice prior to mating and mate exclusion thereafter, while it may be a response to infanticide risk during pregnancy and lactation.
Adapted social behavior allows both individual and collective well‑being. At the individual level, it is a hallmark of health. Indeed, virtually all mental health disorders are associated with social deficits. We are interested in understanding the behavioral, neural, and neurochemical bases of social cognition and communication using mouse models. Here, we will review our recent data showing the crucial role of the prefrontal cortex in the organisation of adapted social interaction, the interplay between the cholinergic and the noradrenergic systems for the balance between affiliative interaction, dominance, and control of aggressiveness, and we will discuss the putative role of ultrasonic communication in social interactions in adult animals. We will see the role played by the environment of life and by the context in which interactions take place in healthy individuals and in pathological situations. Together, the data presented will offer a novel focus on the social brain – and social life – of rodents and provide some practical recommendations for future experiments.
Patrolling behavior plays an important role in resource defense and in shaping social interactions in territorial species. However, it is not clear whether and how resource deterioration affects patrolling and interactions between territorial males. We addressed this issue by studying the territorial patrolling of damselfly Calopteryx splendens males, which use riverine vegetation patches composed of floating rafts of Potamogeton natans as territories. Males can hold single territories established on one vegetation patch (solitary residents) or hold adjacent territories established on shared vegetation patch (contiguous residents). The study predicted that solitary males engage more in patrolling than contiguous residents and that patrolling intensity is proportional to patch quality. Two types of semi-natural vegetation patches were sunk: of high and low quality measured on the basis of the patch size (range 2–5 m2) and its attractiveness to damselflies (measured as number of residents, non-territorial males and contests observed at a given patch). Changes in number of patrolling flights were monitored for solitary and two contiguous residents: first which hold territory situated closer to the patch centre and secondary holding territory nearer to the edge of a patch. Results indicated that solitary residents patrolled more often than either of the two contiguous residents. Habitat deterioration significantly reduced the patrolling intensity of both single and first contiguous resident, however, their patrolling activity was not resumed at the same intensity after the original patch had been restored. The secondary resident of a contiguous pair did not respond to habitat deterioration, but increased its patrolling activity following restoration. Patch quality was found to have no impact on patrolling, which implies that social context can be more important in predicting changes in patrolling behavior in response to resource deterioration.
Changes in behaviours of the two peccary species, Tayassu tajacu Linnaeus, 1758 and T. pecari Link, 1795 between non-receptive and receptive periods were followed by presenting females to males daily for 15 minutes. In T. tajacu, the rank order of behaviours, similar in both sexes during the non-receptive period, differs during receptivity. Contact behaviours decrease in males, whereas sexual ones progress. The same tendency appears in females. Inhibited bites replace markings of partner as the most common behaviour in both sexes. In T. pecari, the rank order of behaviours always differs between sexes. When females become receptive, the differences from the non-receptive period are neither numerous nor significant. The most common behaviour of males, previously markings of partner, becomes mounts, whereas in females agonistic behaviours reinforce their dominance. In this species, the only behaviours that increase are those leading directly to copulation or those of an agonistic nature. In both species, females show more agonistic behaviours than males (mainly inhibited bites in T. tajacu, truly aggressive ones in T. pecari). When females are receptive, males of T. pecari become less active, contrary to T. tajacu where both sexes double their activity. In T. tajacu, most behaviours vary significantly in relation to the progesterone level, contrary to the other species. These pecularities appear correlated to herd composition and organisation.
Antler traits (length of the main beam, circumference of the main beam, coronet circumference, and the number of antler points) in roe deer Capreolus capreolus (Linnaeus, 1758) from a population in Casentino (Northern Tuscany) were examined for associations with genotypes at loci coding for enzymes. Significant associations were found only in yearlings. Individuals homozygous for the allele Mpi120 had significantly larger antlers than carriers of other genotypes at Mpi. Individuals homozygous for Pep-2100 had significantly smaller antlers than carriers of other genotypes at Pep-2. In adults the results were essentially the same, but only as a statistically insignificant trend. The data were interpreted in terms of one or more genetic components having a major influence on antler development only in yearlings. This corresponds with behavioural data suggesting that in adults antler size is not related to reproductive success, but in yearlings it is correlated with sexual maturity and the rate of being expelled from the home range of their mothers by territory holders. The situation in the roe deer was compared with previous results on the genetics of antler development in the red deer, where males are social rather than solitary, yearlings do not participate in reproduction, and some antler characteristics are related to reproductive success of adult stags.
Use of resting sites by 14 radio-tracked pine martens Martes martes (Linnaeus, 1758) (6 males and 8 females) was studied in Białowieża National Park, northeastern Poland. Males used, on average, 191 different resting sites throughout the year while females used 159 sites. The reuse index varied from 0.07 to 0.94 and it was highest for females with young in spring and lowest for both males and females in summer, in autumn-winter, reuse of resting sites by males decreased significantly (p = 0.006) in deep snow cover and it increased (p = 0.021) when marten's ate more ungulate carcasses. In spring, the reuse index for males was negatively correlated with the mean ambient temperature during the tracking period (p = 0.019) and did not depend on rodent abundance. In spring and autumn-winter, most individuals used resting sites which were randomly distributed in their home ranges. In summer, resting sites of males were clumped and were located near the edges of their home ranges, close to females' home ranges. The average distance between consecutively used resting sites was significantly higher for males (690 m) compared to females (419 m, p < 0.001). In spring and autumn-winter (but not in summer) the average distance between the male resting sites depended on their body mass. For males, temperature had significant influence on the pattern of resting site choice for the following day, while for females this relationship was not found. Individuals of the same sex and overlapping home ranges did not use the same resting sites in the same season, except for a young male and an old female that were found in one resting site at the same time from mid-June to mid-August.
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