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Background. Tobacco use is the foremost preventable cause of death and disease in the world today and work strain might be related with nicotine dependence by its provoking effect. Objective. To assess the association between occupational stress and nicotine dependence. Material and methods. A cross sectional survey was conducted among 200 subjects visiting a satellite dental clinic of Swami Devi Dyal Hospital and Dental College, Barwala, Panchkula, India. Nicotine dependence was measured using the Fagerstrom Test for Nicotine Dependence (FTND). The extent of the stress factors experienced at work was assessed using the Effort-Reward Imbalance scale (ERI). Chi-square test and multiple logistic regression analysis were employed for statistical analysis. Confidence level and level of significance were set at 95% and 5% respectively. Results. The results of bivariate analysis revealed association of occupational stress with age, marital status, educational status, level of nicotine dependence, frequency of cigarette smoking and religious attendance. However, multivariate analysis elicited the significant association of occupational stress with only two variables, level of nicotine dependence and frequency of smoking. Conclusion. Occupational stress was found to be associated with nicotine dependence in the present study implying a need of other tranquil measures to be incorporated for the relief of work related stress.
Effects of salting, pickling and smoking processing methods on the mineral composition in fish products were investigated. The concentrations of Ca, P, Mg, K, Na, Cu, Fe, Zn, Mn and Se in fish products were determined during subsequent stages of processing in raw fish, semi-processed products and ready products. Fresh fish contained macro-elements in the following order: K > P > Na > Ca > Mg. The tendency was similar in semi-processed and ready products, except for the dominance of sodium. The sodium content in marinated products was three-fold higher than in fresh fish and twelve-fold higher in salted herring and cold-smoked fish. The sodium content in a 100 g portion of salted herring exceeded by six-fold the recommended daily allowance and by two-fold the tolerated amount. The content of K, Mg and P in marinated and salted herring tended to decrease throughout processing. No significant differences were observed in concentrations of Ca. The order of the concentrations of microelements was as follows: Zn > Fe > Cu > Se > Mn. The content of these elements in fish products was similar to their levels in fresh fish and corresponded to the following shares of the recommended allowances: Se up to 20%; Cu up to 11%; Zn up to 6%; Mn up to 1%; Fe up to 10%. Disadvantageous changes in the mineral composition in fish during processing result from water losses during technological processes, but the primary cause of mineral loss was leaching when the products were soaked in brine solutions with a high sodium content. Significant changes in the content of minerals in salted and pickled herring decreased the nutritional value of these products. Smaller mineral losses that occurred during smoking meant that these products were more valuable nutritionally in relation to minerals.
Background. The aim of the study was to compare chosen aspects of lifestyle of women aged 45-59 and 60-74. Material and methods. The study, carried out between 2013 and 2015, embraced 120 women who engaged in physical activity. Respondents were students of the University of the Third Age in Gorzów Wlkp. and the Elderly and Disabled Activation Center in Piła. Two age categories were distinguished: 45-59 and 60-74 years old. In the study a diagnostic survey was employed, with the use of the techniques of questionnaire, interview and observation. The qualitative and quantitative analysis was carried out with the employment of: frequency of characteristics, chi-squared independence test and multiple correspondence analysis. Results. Older women far more often chose positive health behaviors than those under 60 years of age. There were more current nonsmokers among them, as well as nondrinkers or those who drank less frequently and controlled their health more often. Younger women assessed their own health and physical fitness higher. There were no obese people among the subjects. Conclusions. There is a need for t he dissemination of healthy lifestyle among people aged 60+, especially in the situation of an aging population with unstable retirement age. Activation of people belonging to this age group is a chance to improve their life quality.
The aim of our study was to evaluate cellular content in induced sputum in chronic obstructive pulmonary disease (COPD) in relation to the degree of airway obstruction, macrophage count, and phenotype. We compared the proportion of macrophages and cells expressing the following markers: CD11b, CD14, CD54, and CD71 in induced sputum obtained from patients with mild-to-moderate and severe COPD (n=29)], asymptomatic smokers (n=18), and nonsmokers (n=18). The differential cell count and macrophage phenotypes were examined in induced sputum by immunocytochemistry. We observed a greater proportion of neutrophils and eosinophils and an elevated macrophage count in patients with COPD and in smokers in comparison with nonsmokers. Macrophages in patients with severe airway obstruction were characterized by a significantly elevated expression of CD11b and CD14 markers. There were higher proportions of macrophages with expression of CD11b, CD14, CD54, and CD71 in induced sputum of smokers in comparison with nonsmokers. We concluded that macrophages are the cells involved in the inflammatory process caused by smoking in COPD. The macrophage phenotype with elevated CD11b and CD14 expressions was associated with severe airflow limitation.
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Potential confounding factors in measurement of exhaled nitric oxide

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Nitric oxide is present in the exhaled air. Factors affecting the level of exhaled nitric oxide (exNO), except for smoking, are not well defined. In this study we seek to determine whether age, gender, body mass index (BMI), part of the day, or time after a meal could modulate exNO levels. exNO was examined by the use of a chemiluminescence method in 100 subjects - 31 women (19 nonsmokers and 12 smokers) and 69 males (55 nonsmokers and 14 smokers). Forty four subjects took medications due to stable coronary disease, 22 were after heart transplantation, and 34 did not take any drugs. We found that exNO levels did not differ either between the whole groups of women and men or between smokers and nonsmokers of either respective group (4.91 ±2.38 vs. 6.27 ±4.23 ppb; 3.21 ±1.16 vs. 3.71 ±1.55 ppb; 5.98 ±2.35 vs. 6.92 ±4.45 ppb). The correlation of exNO with age in the whole population was weak (r=0.23; P=0.02) and insignificant in the smoking and nonsmoking subgroups. Likewise, correlations of exNO with BMI, part of the day, or time after a meal were insignificant in whole population as well as the subgroups. We conclude that the aforementioned factors are not able to confound the measurement of exNO in the population studied.
Background. The transition from high school to college is an important milestone in the life of adolescents. In this stage of life, they are already aware of their responsibility for their health, which allows them to develop their health habits. The aim of this study was to increase the knowledge about selected aspects of female university students’ lifestyle, including sports, sleep, stress, alcohol and smoking. Material and methods. This cross-sectional research was carried out with 1,055 female university students in their first year of study at two universities in Slovakia as part of the VEGA grant project no. 1/0825/17 “Recommendations for physical activities in prevention and control of non-communicable diseases and their implementation in the Eastern part of Slovakia” implemented at P.J. Šafárik University in Košice. Data was collected on lifestyle factors, including frequency and regularity of participating in sports during the preceding half year, by means of a questionnaire. Results. Students of both universities reported low levels of engagement in sport activity in the preceding six months, in terms of frequency and regularity of its weekly performance. There appeared to be a statistically significant relationship between the frequency of sport activity and fatigue after waking. Other relationships were not found to be statistically significant. Conclusions. The scope of educational work by university education experts should include appropriate ways of influencing the lifestyle of first-year university students towards maintaining their good health. This can be carried out within the context of health education during compulsory and optional physical education classes.
The aim of the study was an evaluation of changes in protein level and activity of SOD isoenzymes, and the participation of AP-1 and NF-κB in subsequent stages of colorectal cancer development. Studies were conducted on 65 colorectal cancers. Controls were unchanged colon regions. Activity of SOD isoenzymes, lipid peroxidation level (TBARS), and protein level of SOD1, SOD2, AP-1 and NF-κB were determined. We found that the protein level and activity of SOD isoenzymes and protein level of AP-1 and NF-κB change in subsequent stages of clinical advancement of colorectal cancer, according to UICC (I-IV), and in grades of tumor cells differentiation (G1-G3). These results indicate adaptation of colorectal cancer cells to oxidative stress, and show that the observed changes of SOD activity and protein level depend on gradual progression of colorectal cancer, and suggest an impairment of processes regulated by AP-1 and NF-κB which are critical for tumor progression (proliferation, differentiation and apoptosis).
Background. Stability and high bioaccumulation coefficients of polychlorinated biphenyls (PCB) in aqueous organisms may constitute a health hazard for their consumers (it is really the potential toxicity of PCBs that determine their hazard—not stability). As the fish are mostly eaten in the form of processed products, the influence of technological processes and different methods of cooking on changes in the levels of toxic compounds in final products is of great importance and may be helpful for appropriate processing. Since smoked fish products are in great demand in Poland, the goal of this study was to determine changes in total PCB content in herring fillets during hot smoking and in hot and cold smoked mackerel fillets. Materials and Methods. Herring fillets were hot smoked under industrial conditions, whereas mackerel fillets were cold- and hot smoked. Analyses were carried out using a capillary gas chromatograph coupled with a mass spectrometer GC-MS (HP 6890/5973) with a HP-5 column. Results. The hot smoking process affected the loss of total PCB content in mackerel and herring fillets, with its highest value in the first hour of partial drying. The main factor determining the changes in the content of these compounds in wet matter and lipids was their escape in co-distillation with water vapour. The increase of their content, in the final stage of hot smoking and during the whole cold smoking process, was caused by contamination of fish fillets with polychlorinated biphenyls present in the curing smoke. Conclusion. From the toxicological point of view the process of hot smoking, is much healthier for people because it causes the reduction of total PCB content in final smoked products. Cold smoked mackerel is much more popular on the Polish market for its stronger taste and aromatic smell. However, lengthy exposure to smoke in low temperature increased the total PCB content in cold smoked fish. The consumption of these products should be limited based on these results.
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