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Characterization of a nucleopolyhedrovirus isolated from the laboratory rearing of the beet armyworm Spodoptera exigua [Hbn.] in Poland

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Jakubowska A., Vlak J. M., Ziemnicka J.

Journal of Plant Protection Research

2005

tom 45

nr 4

279-286

A nucleopolyhedrovirus isolated from the beet armyworm Spodoptera exigua (Polish laboratory culture), SeMNPV (P), morphologically similar to the viral bioinsecticide virus Spod-XR , was characterized molecularly and biologically. Phylogenetic analysis based on three conserved baculovirus genes, polh, lef-8 and pif-2, showed the highest homology of SeMNPV (P) to Mamestra brassicae (Mb) MNPV and M. configurata (Maco) MNPV, and much less to SeMNPV (Spod-XR). These findings were confirmed by genomic DNA restriction profile analyses. Bioassays revealed that SeMNPV isolated from the commercial bioinsecticide Spod-XR was themost infectious for S. exigua, while the infectivity of SeMNPV (P) and MbMNPV was significantly lower. These data suggest that SeMNPV (P) is a variant of MbMNPV.

Ograniczanie wyników

1 Journal of Plant Protection Research

1 Jakubowska A.

1 Vlak J. M.

1 Ziemnicka J.

1 2005

Wyniki wyszukiwania

Characterization of a nucleopolyhedrovirus isolated from the laboratory rearing of the beet armyworm Spodoptera exigua [Hbn.] in Poland