The aim of the study was to evaluate the fatty acids composition of storage fat of slaughter horses. Samples of cervical and peri-intestinal fat taken from horses of different genders and weights in a slaughterhouse were used as the study material. The fat was esterified by the method described by Peisker. Separation and analysis of the fatty acids was conducted by gas chromatography with a flame ionisation detector. The samples were found to contain fatty acids with chains of 14 to 20 carbon atoms, mainly palmitic, myristic, oleinic, palmitoleic and linolenic acids. Small differences were found between groups of horses classed by body weight. The experiment confirmed that the composition of horse fat in terms of mono- and polyunsaturated acid content is interesting from a nutritional point of view. Cervical fat contained average 49% of monounsaturated fatty acids and polyunsaturated fatty acids in horses in mass to 550 kg and mass above 550 kg 12.57% and 10.79%, respectively.
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