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Y. enterocolitica infection of pigs is concerned with a common carrier and shedding states in this species. The aim of the study was to determine the influence of the experimental immunization of pigs with a selected Y. enterocolitica strains suspension on the controled infection course, the antibody level formation, as well as the duration of pathogen shedding. The immunization of pigs was enforced with a suspension of Y. enterocolitica strains previously inactivated with formol, suspended in PBS and demonstrated in vitro high immunogenicity in Respiratory Burst Activity/Potential Killing Activity (RBA/PKA) and Mitogen Transformation Test (MTT). The study was performed on 15 pigs divided into 3 groups. The animals from groups I and II received an immunizate with a density of 2.7 × 10⁹ cfu/ml administered subcutaneously in doses 2 ml and 5 ml, respectively, twice in a 2-week interval. The third group (control) was administered PBS in an analogous scheme. The evaluation in vivo was conducted after a per os challenge with a pathogenic strain of Y. enterocolitica O:3. The first and booster immunization had no effect on the clinical picture and body weight gains of the immunized animals. The fastest and strongest immune response to the per os challenge with Y. enterocolitica O:3 was observed in the control group, already in the first week post infection (wpi). Higher antibody levels were found in the group of animals where 5 ml 2.7 × 10⁹ cfu/cm³ subcutaneously were administered. In the first wpi bacterial shedding was observed in all animals that belonged to group I and the control group which persisted up to 3 wpi. Among the pigs from group II the shedding was observed in 3 out of 5 animals, and which finished in 6 days post infection. Applied experimental immunization against per os challenge with a pathogenic strain of Y. enterocolitica O:3 did not prevent pathogen shedding, but merely limited its intensity and duration.
The article reviews the importance of pigs, pork and pork products as a source of food poisoning in humans caused by non-typhoidal Salmonella serovars, particularly Salmonella Typhimurium. Even in countries with highly competent veterinary services and satisfactory financial resources, symptomless carriers of Salmonella organisms are provided to the slaughter house by a high percentage of swine herds These are the primary source of secondary contamination of food products and ultimately of food poisoning in humans as well as infection by antibiotic-resistant strains capable of genetic transfer of these properties to bacteria of the human flora. Bearing in mind the abovementioned risk of infecting humans by Salmonella organisms, the fundamental components of veterinary prophylactic activity are the provision of satisfactory biosecurity and welfare to swine at the farm level combined with periodical disinfection and monitoring programs for Salmonella carriership of swine. This is continued by preventing additional spreading of Salmonella infection among swine during transportation to the slaughter house and while waiting for culling. Unfortunately, vaccination of swine in the farm against Salmonella is ineffective in decreasing carriership of non-typhoidal Salmonella serovars. Also the method of competitive exclusion for interfering with Salmonella colonization of the swine intestine proved unsatisfactory. An essential step is the prevention of secondary contamination of pork and pork products before human consumption. Bearing in mind the significance of Salmonella carriership in swine, as important food animals, for public health, further research for elaborating more effective procedures of preventing the carriership of non-typhoidal Salmonella is recommended. This review presents data on the mechanisms of Salmonella colonization of the swine intestine.
This article discusses different effects of pathogenicity of Chlamydiaceae in animals, with particular reference to cattle. The pathogenicity for humans is mentioned as well. It is pointed out that chlamydioses caused by individual species of Chlamydiaceae are avian chlamydiosis, caused by Chlamydophila psittaci, ovine chlamydiosis, caused by Chlamydophila abortus, and trachoma in humans, caused by Chlamydia trachomatis. More often these and other species of Chlamydiaceae are involved in mixed infections with other facultatively pathogenic microorganisms in different species of animals, such as cattle (including calves), causing respiratory disorders, enteritis, abortion, mastitis, polyarthritis, and encephalomyelitis. Subclinical infections in which Chlamydiaceae behave as commensals occur rather frequently. Since diagnosis of chlamydial infections requires the application of laboratory assays, the article presents and evaluates selective modern tests for the identification of microorganisms or specific antibodies, particularly ELISA and RT-PCR. Serological detection has been found more suitable for prevalence surveys than for retrospective diagnosis of chlamydial infection. Chlamydophile psittaci followed by Chlamydophila abortus and Chlamydphila pecorum are mentioned as the most prevalent representative species of Chlamydiaceae in cattle. Fecal shedding of Chlamydiaceae by carrier animals is thought to be the most important way of spreading the infection. In most cases in which pathological syndromes are observed in cattle, representatives of Chlamydiaceae are among several factors in a polyetiological complex, which also includes other species of microorganisms and unfavourable environmental conditions. Therefore, an improvement in herd management, and especially in the housing and nutrition of animals, is of primary importance for the control of chlamydial infections. There is no evidence of a successful use of antimicrobials in the elimination of bovine chlamydial infection. The use of vaccines is recommended. Their efficacy, however, needs to be improved.
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