Ograniczanie wyników

Czasopisma help
Autorzy help
Lata help
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 27

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  siderophore
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
Rhizobium strains isolated from nodules of the different legumes including wild-growing plants were examined for their siderophore activity. Fifteen of the 84 screened rhizobial strains were able to grow under conditions of limited iron supply. Nine of them gave orange halos in the assay with Chrom azurol S. Among these strains were Rhizobium sp. (Ononis) and Rhizobium (Genista), producing hydroxamates and phenolates. These compounds could promote the growth of siderophore-negative bacteria on iron-deficient media. The results imply that the hydroxamates from G1 and Ol strains may belong to the monohydroxamate class of siderophores.
Fluorescent pseudomonads are among the most influencing plant growth-promoting rhizobacteria in plants rhizosphere. In this research work the plant growth-promoting activities of 40 different strains of Pseudomonas fluorescens and Pseudomonas putida, previously isolated from the rhizosphere of wheat (Triticum aestivum L.) and canola (Brassica napus L.) and maintained in the microbial collection of Soil and Water Research Institute, Tehran, Iran, were evaluated. The ability of bacteria to produce auxin and siderophores and utilizing P sources with little solubility was determined. Four strains of Wp1 (P. putida), Cfp10 (Pseudomonas sp.), Wp150 (P. putida), and Wp159 (P. putida) were able to grow in the DF medium with ACC. Thirty percent of bacterial isolates from canola rhizosphere and 33% of bacterial isolates from wheat rhizosphere were able to produce HCN. The results indicate that most of the bacteria, tested in the experiment, have plant growth-promoting activities. This is the first time that such PGPR species are isolated from the Iranian soils. With respect to their great biological capacities they can be used for wheat and canola inoculation in different parts of the world, which is of very important agricultural implications.
The inhibition of 23 strains of actinomycetes by 7 strains of fluorescent pseudomonads and vice versa, on 4 different media, was examined. The antagonism of pseudomonads was more frequent and more intense than that of actinomycetes. Inhibition was observed in pseudomonads and actinomycetes of both kinds of strains, antagonistic and non-antagonistic to Fusarium oxysporum. Some media were more favorable to the an-tagonism of pseudomonads and others to that of actinomycetes, hence in identical environmental conditions mutual antagonism between the two counter-partners occurred only rarely.
Fluorescent Pseudomonas (FP) is a major group of plant growth promoting rhizobacteria and a well-known synthesizer of siderophores, which imparts a selective advantage on rhizosphere competence and their biocontrol traits. The present study was aimed at examining the factors affecting the production of siderophores and their potential biocontrol traits. Sixteen FP isolates were shortlisted based on their siderophore-producing ability in chrome azural S medium. The isolates were checked for variations in siderophore production under varying incubation times, temperatures, pH, iron (Fe3+) concentrations and mutagens. In addition, the iron binding affinity of siderophores, mycelial inhibition assay and plant growth promotion traits were assessed. Results showed that the siderophore production was highly influenced by the time of incubation, changes in pH, temperature and iron concentration. Chemical characterization showed that the produced siderophores were hydroxamates. Maximum siderophore production was observed at pH 7 whereas UV and EtBr exposure invariably suppressed siderophore production drastically in all isolates. All FPs from maize rhizosphere showed excellent siderophore production which could be due to the competence in strategy-II of the plant rhizosphere and significant growth inhibition on Fusarium oxysporum. Our results suggest the inclination of siderophores to iron, in terms of various criteria affecting production and the possible role of environmental mutations that affect the natural iron harvesting mechanism.
We examined the interaction between the roots of Pinus sylvestris and closely related species Heterobasidion annousum s.l. (H. annosum s.s., H. parviporum, H. abietinum) that differ in host plant preference. The aim of the current study was to determine in roots the accumulation pattern of low molecular mass compounds such as catecholate and hydroxamate derivates, oxalic acid as well as iron-reduction ability of that low molecular mass compounds, that play important roles in wood degradation and they are also involved in pathogenesis. The accumulation of catechol and hydroxamate derivates increased during the early (2–6 h) and late (24–48 h) stages of interaction and similar pattern of oxalic acid accumulation were observed. The level of catecholate derivates in P. sylvestris roots that were challenged with H. parviporum or H. abietinum correlated strongly with iron reducing ability. However, when host was exposed to H. annosum s. s. hydroxamates rather than catecholates regulated iron reducing ability. The extracellular Fe3+ reducing activity was greater for H. annosum s. s. isolates than for isolates of two other species, and reduction of ferric iron may promote oxidative burst in host cell and fungal colonization. Catecholate concentration in the presence of H. annosum s.s. contributing to host cell death, confirm iron involvement in infection success.
Only 9 (11.2%) out of 80 studied bacterial strains were able to utilize iron saturated 2-oxo acids and hydroxyacids and grow on o-phenantroline containing media. These strains belonged to Enterococcus faecalis and Enterococcus faecium species and were isolated from clinical material. Iron sources utilized by all of these strains were Fe(III) complexes with pyruvic, 2-oxobutyric, 4-methylthio-2-oxobutyric, 2-oxo-3-methylva!eric, 2-oxoisocaproic and 2-oxoadipic acids. None of the nine strains released 2-oxoacids to environment during growth in iron excess Fe⁺ medium and iron deficient - Fe⁻ (Chelex) medium. In Fe⁻ (phenantroline) medium, when the growth was strongly inhibited, only pyruvic acid was released. Iron uptake from ⁵⁹Fe(III)-pyruvate was depended on iron deficiency during growth: cells harvested from Fe⁻ (phenantroline) medium have acquired the most amount of iron. 2,4-Dinitrofenol was a strong inhibitor of ⁵⁹Fe(III) iron uptake. Release of pyruvic acid is not subject to negative derepression and does not require the presense of iron as its inductor. It appears in the environment as a response to growth inhibiting stress caused by the iron deficiency but contrary to siderophores are not specially synthesized for iron assimilation. Therefore, it is only primary metabolism products released by damaged, but metabolic active cells.
In Staphylococcus aureus B47 grown in iron-restricted medium, six new, iron-regulated proteins occurred in cytoplasmic membrane. Protein of 14kDa has bound two complexes of iron: Fe(III)-staphylobactin and Fe(III)-acinetoferrin. Complexes of Fe(III)-ferrichrome and Fe(III)-rhodotorulic acid were not bound to any of new membrane protein. Iron of Fe(III)-staphylobactin and Fe(III)-acinetoferrin complexes was transported into the cells.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.