Wyniki wyszukiwania

1

Serum paraoxonase activity and total sialic acid in sheep with foot and mouth disease

100%

Deveci H. A., Kukurt A., Nur G., Alpay M., Merhan O., Bozukluhan K., Yilmaz V., Karapehlivan M.

Medycyna Weterynaryjna

|

2018

|

tom 74

|

nr 03

The aim of this study was to investigate paraoxonase (PON1) activity and total sialic acid to be measured for the first time in sheep infected naturally with foot and mouth disease, and their relationships with oxidative stress. A total of 30 Awassi sheep (aged between 2-4), which were healthy and infected with the foot and mouth disease virus (FMDV) were used in the study. Concentrations of paraoxonase activity (PON1), which is an important antioxidant against oxidative stress, high density lipoprotein (HDL) and total sialic acid (TSA), which has a critical role for immune system and is one of the significant indicators of cellular degeneration, were measured in serum samples drawn from animals. The total oxidant capacity (TOC) and total antioxidant capacity (TAC) were evaluated to determine the oxidative balance. It was observed that serum PON1 (P < 0.001) and HDL (P < 0.01) concentrations were significantly lower compared to the control group. TSA concentration was higher in the infected group (P < 0.001) compared to the healthy group. TOC was higher (P < 0.001) and TAC was lower (P < 0.01) in the infected group compared to the control group. Consequently, harmful effects of the infection of foot and mouth disease were shown as cause of oxidative cell damage and the high rate of sialic acid was produced depending on the degeneration during the infection.

2

The role of target membrane sialic acid residues in the fusion activity of the influenza virus: the effect of two types of ganglioside on the kinetics of membrane merging

100%

Ramalho-Santos J., Pedroso De Lima M. C.

Cellular and Molecular Biology Letters

|

2004

|

tom 09

|

nr 2

The influenza virus enters target cells via the action of hemagglutinin proteins (HA) inserted into the viral envelope. HA promotes membrane fusion between the viral envelope and endosomal membrane at low pH, following viral binding to sialic acid-containing receptors on target cells, and internalization by endocytosis. The effect of target membrane sialic acid residues on the fusion activity of the influenza virus towards model membranes was evaluated by both reduction, (i.e. treating somatic cells with neuraminidase- (NA-) prior to virus-cell interactions), and by supplementing liposomes with the gangliosides GD1a and GT1b. The harshness of the neuraminidase pretreatment of target cells required to affect virus-induced membrane merging was found to greatly depend on the assay conditions, i.e. whether a virus-cell prebinding step at neutral pH was included prior to acidification. Minor concentrations of neuraminidase were found to greatly reduce virus fusion, but only in the absence of a prebinding step; they had no effect if this step was included. Although membrane merging was greatly reduced following cell neuraminidase pretreatment, virus-cell association at low pH was not disturbed proportionately. This probably reflects unspecific virus-cell binding under these conditions, probably of inactivated or aggregated virus particles, which does not translate into membrane merging. This seems to suggest both that target membrane sialic acid can protect the virus from losing its activity before triggering membrane merging, and that the importance of this interaction is not merely to ensure virus-target proximity. With liposomes, we found that both types of ganglioside supported efficient fusion, with GD1a promoting a slightly faster initial rate. However, in this case, virus-target proximity closely mirrored fusion activity, thus pointing to differential specificity between targets routinely used to assay influenza virus fusion activity.

3

The interaction between serotonin and n-acetylneuraminic acid or 2-keto-3-deoxyoctulosonic acid

86%

Rybka J., Gamian A.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

4

Apoptosis of lymphocytes and sialic acid plasma level in patients undergoing cardiac surgery

86%

Wysocka A., Korycinska A., Dragan M., Berbec H., Rolinski J., Stazka J.

Folia Biologica

|

2005

|

tom 53

|

nr 3-4

5

The effect of carbohydrate moiety structure on the immunoregulatory activity of lactoferrin in vitro

72%

Zimecki M., Artym J., Kocieba M., Duk M., Kruzel M. L.

Cellular and Molecular Biology Letters

|

2014

|

tom 19

|

nr 2

The aim of this study was to evaluate the immunoregulatory effects of recombinant human lactoferrin (rhLF) in two in vitro models: (1) the secondary humoral immune response to sheep erythrocytes (SRBC); and (2) the mixed lymphocyte reaction (MLR). We compared the non-sialylated glycoform of rhLF as expressed by glycoengineered Pichia pastoris with one that was further chemically sialylated. In an earlier study, we showed that sialylated rhLF could reverse methotrexate-induced suppression of the secondary immune response of mouse splenocytes to SRBC, and that the phenomenon is dependent on the interaction of lactoferrin (LF) with sialoadhesin (CD169). We found that the immunorestorative activity of sialylated rhLF is also dependent on its interaction with the CD22 antigen, a member of the immunoglobulin superfamily that is expressed by B lymphocytes. We also demonstrated that only sialylated rhLF was able to inhibit the MLR reaction. MLR was inhibited by bovine lactoferrin (bLF), a glycoform that has a more complex glycan structure. Desialylated bLF and lactoferricin, a bLF-derived peptide devoid of carbohydrates, did not express such inhibitory activity. We showed that the interaction of LF with sialic acid receptors is essential for at least some of the immunoregulatory activity of this glycoprotein.

6

Changes of the expression of Lewis blood group antigens in glycoproteins of renal cancer tissues

72%

Borzym-Kluczyk M., Radziejewska I.

Acta Biochimica Polonica

|

2013

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tom 60

|

nr 2

Sialic acid and sialyl Lewisa/x are found on N- and O-glycans of many human malignant cells. Carbohydrate antigens can be used as tumor markers, and an increase of their levels in cancer cells is associated with tumor progression. The aim of this study was to assess the level of some Lewis blood group antigens on glycoproteins in tumor (cancer tissue), intermediate zone (adjacent to tumor tissue), and normal renal cortex/medulla (uninvolved by tumor). The study was performed on tissues taken from 30 patients. Relative amounts of sugar structures of proteins with molecular masses above 30 kDa were determined by ELISA-like test with biotinylated lectins: MAA (Maackia amurensis), SNA (Sambucus nigra), and monoclonal antibodies anti-sialyl Lewisa/x. Higher expression of all examined structures was revealed in cancer tissues. Significant increases were observed for sialic acid linked α 2-3 in cancer tissues when compared to healthy ones and also among intermediate and healthy tissues. The sialic acid linked α 2-6 and sialyl Lewisx structures were significantly increased in cancerous cells when compared to normal and intermediate renal tissue. In case of sialyl Lewisa antigen, a significant difference was discovered between normal and intermediate tissue. Our results confirm that the examined Lewis antigens can be involved in tumor development. Their increase in cancer tissues can suggest their specific role in the process.

7

Elevated serum total sialic acid concentrations in sheep with peste des petits ruminants

72%

Yarim G. F., Nisbet C., Yazici Z., Okur Gumusova S.

Medycyna Weterynaryjna

|

2006

|

tom 62

|

nr 12

1375-1377

Peste des petits ruminant (PPR) is a highly contagious and economically important disease of small ruminants. Plasma sialic acid is a marker of the acute phase response in pathological conditions and significant alterations within total sialic acid serum (TSA) concentrations have been documented in various diseases. The purpose of the study was to investigate changes in TSA serum concentrations in sheep suffering from peste des petits ruminants. Eighty sheep sera were tested for antibodies against PPRV with c-ELISA and 22 of the 80 sera (27.5%) were found to be positive. Twenty two sheep naturally infected by PPRV aged between 1-2 years old were categorized as the infected group and 16 clinically healthy sheep of the same age constituted the control group. Venous blood was sampled from the sheep's jugular veins. The concentration of TSA serum was measured by Warren's thiobarbituric acid assay. Clinical, hematological and biochemical changes, including liver function tests, were also evaluated. Higher concentrations of TSA serum were found in the infected sheep (84.7±13.1 mg/dL) compared to the healthy sheep (63.5±4.7 mg/dL), (P£0.001). In addition, significant correlations were determined between TSA and clinical symptoms, hematological changes and liver function tests of the infected sheep. The findings of the study indicate that TSA plays a part in the disease processes of PPR and that determining TSA serum concentrations may be used as a supplementary laboratory test in conjunction with clinical and laboratory findings when evaluating the prognosis of PPR.

8

How influenza's neuraminidase promotes virulence and creates localized lung mucosa immunodeficiency

72%

Bhatia A., Kast R. E.

Cellular and Molecular Biology Letters

|

2007

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tom 12

|

nr 1

Neuraminidase (NA) is an enzyme coded for by the genome of influenza critical for its pathogenicity and survival. Three currently accepted roles for this NA in promoting influenza virulence are: 1. NA cleaves newly formed virus particles from the host cell membrane. Without NA, newly formed virus would remain attached to the cell within which it was produced. 2. NA prevents newly released virus particles from aggregating to each other, preventing clumping that would reduce dissemination. 3. NA promotes viral penetration of sialic acid-rich mucin that bathes and protects respiratory epithelium through which the virus must spread and replicate. We outline here previous research evidence of two further, albeit hypothetical, functions of NA that together could cause disruption the mucosa-IgA axis, creating localized partial immunosuppressed state, enhancing both influenza infection itself and secondary bacterial pneumonia: 4. IgA provides primary immunoglobulin defense of mucosal surfaces. The hinge region of IgA is normally sialylated. IgA denuded of sialic acid is recognized, bound, and cleared by hepatic asialoglycoprotein receptor (ASGPR). Thus, IgA exposed to free NA would be so denuded and have increased hepatic clearance. 5. NA removes sialic acid moieties from mucosa-residing gamma/delta T cells or IgA producing B cells. Previous work indicates desialylation of these lymphocytes' outer cell membrane results in altered homing, to bone marrow, away from mucosa. Currently marketed NA inhibitors oseltamivir (Tamiflu) and zanamivir (Relenza) are FDA approved in USA for influenza prophylaxis and treatment. These NA inhibitors lower incidence of secondary bacterial infection in cases where an influenza infection occurs despite their use. Moreover, they are ameliorative in patients with secondary bacterial infections treated with antibiotics, a benefit that surpasses the treatment of antibiotics alone. We interpret these last two points as indicating our ascription of localized immunosuppression to influenza's NA could be correct and lead to new treatments of infections generally.

9

Serum sialic acid, malondialdehyde, retinol, zinc, and copper concentrations in dairy cows with lameness

72%

Seyrek K., Yaylak E., Aksit H.

Bulletin of the Veterinary Institute in Puławy

|

2008

|

tom 52

|

nr 2

Concentrations of serum sialic acid, malondialdehyde (MDA), retinol, zinc, and copper were measured in 52 lactating Holstein cows. Out of them, 15 were controls, 21 mildly lame, 7 moderate lame, and 9 severely lame. The animals were housed in free-stall barns with a slurry surface and were not released for pasture feeding. Compared to control animals, the mean serum total sialic acid concentrations in animals with severe lameness, but not in those with mild and moderate lameness, were increased significantly (P<0.01). The levels of MDA were higher in severely lame animals than in controls as well as in mildly and moderately lame animals and the differences were statistically significant (P<0.001). Contrary to the elevated serum concentrations of sialic acid and MDA in severely lame cows, the mean serum retinol value of these animals showed a remarkable decline (P<0.05). As for serum zinc and copper concentrations, there were statistically no significant alterations between the groups. In conclusion, results of the present study indicate that the concentrations of serum total sialic acid, MDA and retinol are associated with a degree of the lameness.

10

Bactericidal activity of normal bovine serum [NBS] directed against some Enterobacteriaceae with sialic acid-containing lipopolysaccharides [LPS] as a component of cell wall

72%

Bugla-Ploskonska G., Doroszkiewicz W.

Polish Journal of Microbiology

|

2006

|

tom 55

|

nr 3

The sensitivity of bacteria to the bactericidal activity of serum depends on the structure and organization of the bacterial outer membrane. Sialic acid has been found in the O-specific region of bacterial lipopolysaccharide (LPS) and it plays an essential role in protecting Gram-negative bacteria against the bactericidal activity of human and animal serum. The susceptibility of Gram-negative bacilli with sialic acid-containing LPS to the bactericidal action of normal bovine serum (NBS) was determined. The examined strains (Escherichia coli O104 (PCM 270), E. coli O24 (PCM 195), E. coli O56 (PCM 2372), Citrobacter braakii 037 (PCM 2346) and Salmonella entérica ssp. entérica serovar Toucra 048 (PCM 2359) showed variable sensitivity to the bactericidal effect of the serum. The role of the mechanisms of complement activation in the killing process was also determined.

11

Comparative study of sialidase activity and Gm3 content in B16 melanoma variants with different metastatic potential

72%

Sawada M., Moriya S., Shineha R., Satomi S., Miyagi T.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 2

We previously demonstrated that transfection of a sialidase cDNA into B16-BL6 cells, a highly metastatic and invasive cell line derived from the mouse B16 melanoma, resulted in a marked suppression of metastasis accompanied by decreased cellular content of the GM3 that is one of the target molecules of the sialidase expressed (Tokuyama et al., 1997 Int. J. Cancer, 73, 410-415). To obtain further insight into the involvement of sialidase in metastasis, we made a comparison of the levels of sialidase activity and GM3 content between B16 melanoma cell lines with low (B16-F1) and high (B16-F10 and -BL6) metastatic potential. The cells exhibited sialidase activity towards 4-methylumbelliferyl N-acetylneuraminic acid (4MU-Neu5Ac) and gangliosides at acidic pH in the particulate fractions, but not in the cytosol. The activity toward 4MU-NeuAc was significantly lower in highly metastatic cells. The activity toward gangliosides, on the other hand, varied independently of metastatic potential: B16-F10 cells with a high potential for experimental metastasis showed the lowest level and B16-BL6 cells having high invasiveness had rather a higher level of ganglioside sialidase along with a much greater GM3 synthase activity than the other two cell lines. Flow cytometric analysis with anti-GM3 antibody revealed that highly metastatic cell lines were higher in the binding affinity as compared to B16-F1 cells, B16-BL6 cells containing twice as much cellular GM3 as B16-F1 cells on thin-layer chromatography.

12

Epitope dissection of receptor-active gangliosides with affinity for Helicobacter pylori and influenza virus

72%

Miller-Podraza H., Larsson T., Nilsson J., Teneberg S., Matrosovich M., Johansson L.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 2

Receptor-active gangliosides with affinity for Helicobacter pylori and influenza virus were chemically modified and analyzed by negative ion fast atom bombardment mass spectrometry (FAB MS) or electron ionization mass spectrometry (EI MS) after permethylation. Derivatizations included mild periodate oxidation of the sialic acid glycerol tail or conversion of the carboxyl group to primary alcohol or amides. The modified gangliosides were then tested for binding affinity using thin-layer plates overlaid with labeled microbes or microbe-derived proteins. Mild periodate oxidation, which shortens sialic acid tail without destruction of sugar cores, abolished or drastically reduced binding of H. pylori and avian influenza virus to sialyl-3-paragloboside (S-3-PG). The same effect was observed in the case of binding of the human influenza virus to receptor-active gangliosides of human leukocytes. Conversion of S-3-PG or leukocyte gangliosides to primary alcohols or amides also abolished the binding. However, mild periodate oxidation had no effect on binding of NAP (neutrophil-activating protein of H. pylori) to the active ganglioside.

13

Purification and characterization of GlcNAc-6-P 2-epimerase from Escherichia coli K92

72%

Ferrero M. A., Martinez-Blanco H., Lopez-Velasco F. F., Ezquerro-Saenz C., Navasa N., Rodriguez-Aparicio L. B.

Acta Biochimica Polonica

|

2007

|

tom 54

|

nr 2

N-Acetylmannosamine (ManNAc) is the first committed intermediate in sialic acid metabolism. Thus, the mechanisms that control intracellular ManNAc levels are important regulators of sialic acid production. In prokaryotic organisms, UDP-N-acetylglucosamine (GlcNAc) 2-epimerase and GlcNAc-6-P 2-epimerase are two enzymes capable of generating ManNAc from UDP-GlcNAc and GlcNAc-6-P, respectively. We have purified for the first time native GlcNAc-6-P 2-epimerase from bacterial source to apparent homogeneity (1 200 fold) using Butyl-agarose, DEAE-FPLC and Mannose-6-P-agarose chromatography. By SDS/PAGE the pure enzyme showed a molecular mass of 38.4 ± 0.2 kDa. The maximum activity was achieved at pH 7.8 and 37oC. Under these conditions, the Km calculated for GlcNAc-6-P was 1.5 mM. The 2-epimerase activity was activated by Na++ and inhibited by mannose-6-P but not mannose-1-P. Genetic analysis revealed high homology with bacterial isomerases. GlcNAc-6-P 2-epimerase from E. coli K92 is a ManNAc-inducible protein and is detected from the early logarithmic phase of growth. Our results indicate that, unlike UDP-GlcNAc 2-epimerase, which promotes the biosynthesis of sialic acid, GlcNAc-6-P 2-epimerase plays a catabolic role. When E. coli grows using ManNAc as a carbon source, this enzyme converts the intracellular ManNAc-6-P generated into GlcNAc-6-P, diverting the metabolic flux of ManNAc to GlcNAc.

14

Lipopolysaccharide changes sialylation pattern in the mouse central nervous system

58%

Wielgat P., Holownia A., Braszko J. J.

Journal of Physiology and Pharmacology

|

2012

|

tom 63

|

nr 5

15

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The role of complement activity in the sensitivity of Salmonella O48 strains with sialic acid-containing lipopolysaccharides to the bactericidal action of normal bovine serum

58%

Bugla-Ploskonska G., Futoma-Koloch B., Rybka J., Gamian A., Doroszkiewicz W.

Polish Journal of Veterinary Sciences

|

2010

|

tom 13

|

nr 1

53-62

Sialic acids are important constituents of animal tissue glycoconjugates and are also present in the antigens of some bacterial strains. Capsular polysaccharides with sialic acid (NeuAc) have been extensively studied with regard to sensitivity to the bactericidal action of serum, whereas little is known in this regard about lipopolysaccharides (LPS) which contain NeuAc. Strains of Salmonella 048, able to infect animals and containing the same structures of LPS with NeuAc, were examined for their susceptibility to the bactericidal action of normal bovine serum (NBS). The strains showed varied sensitivity to the bactericidal action of NBS, which indicates that the expression of LPS containing NeuAc residues is not critical for the strains' resistance to the serum's activity. In this study the mechanisms of complement activation responsible for killing serum-sensitive Salmonella 048 rods by NBS were also established. Three such mechanisms were distinguished: activation of the classi- cal/lectin pathways, important (decisive) in the bactericidal mechanism of complement activation, parallel activation of the classical/lectin and alternative pathways, and independent activation of the classical and lectin or the alternative pathway.

16

Similarity of vitamin D-binding protein (DBP) between various animal species in double immunodiffusion and western blotting

58%

Madej J. P., Boratynski J., Nowacki W.

Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine

|

2007

|

tom 10

|

nr 3

17

Purification and characterization of avian glycolipid: beta-galactosyltransferases (GalT-4 and GalT-3): cloning and expression of truncated betaGalT-4

58%

Basu S. S., Dastgheib S., Ghosh S., Basu M., Kelly P., Basu S.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 2

Acidic glycolipid of ganglio-(containing sialic acid) and sialyl-lactofucosyl-type, SA-Lex (containing sialic acid and fucose) are developmentally regulated and appear to be ubiqitous on neuronal and cancer cell surfaces of animals. Two glycolipid: β-galactosyltransferases, GalT-3 and GalT-4, were characterized in embryonic chicken brain (ECB). Based on substrate competition experiments, these two activities were believed to be due to expression of two gene products. A cDNA fragments (about 600 bp) encoding the catalytic domain of the GalT-4 (UDP-Gal:LcOse3Cer β1,4galactosyltransferase) from ECB and human Colo-205 were isolated. These cDNAs were expressed as a soluble glutathione-S-transferase fusion protein (48 kDa) in Eschericchia coli. Interactions between GlcNAc-, UDP-hexanolamine-, and α-lactalbumin were studied with the purified fusion protein (recombinant and truncated). Functionally it was similar to that of native GalT-4 purified (40000-fold) from 11-day-old ECB. GalT-3 (UDP-Gal:GM2β1,3galactosyltransferase) was purified from 19-day-old ECB, and a polyclonal antibody was produced against the peptide backbone for immunoscreening of a λZAP ECB cDNA expression library. Each of the GalT-3 peptides (62 and 65 kDa was analyzed by protein fingerprinting analysis indicating a similar peptide mapping pattern.

18

In comparison to progenitor platelets, microparticles are deficient in GpIb, GpIb-derived carbohydrates, glycerophospholipids, glycosphingolipids, and ceramides

58%

Zdebska E., Wozniak J., Dzieciatkowska A., Koscielak J.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 2

Activated blood platelets shed microparticles with procoagulant activity that probably participate in normal hemostasis. We have isolated spontaneously formed microparticles from human blood and analysed them for ultrastructure, antigenic profile, and biochemical composition. In transmission electron microscopy microparticles appeared as regular vesicles with a mean diameter of 300 nm (50-600 nm). In flow cytometry almost all microparticles reacted with fluorescein isothiocyanate (FITC) labeled antibody to platelet glycoprotein complex IIb-IIIa (GpIIb-IIIa) and with FITC-annexin V but only 40-50% of microparticles reacted with FITC-antibody to platelet glycoprotein Ib (GpIb). The latter result was confirmed by double labeling of microparticles with FITC-antibody to GpIIb-IIIa and phycoerythrin (PE) labeled antibody to GpIb. Large microparticles reacted better with anti-GpIb than the small ones. A decreased level of GpIb was also demonstrated by SDS/polyacrylamide gel electrophoresis of microparticles. Compositional studies indicated, that in terms of cholesterol and protein contents, microparticles resembled platelets rather than platelet membranes as previously thought. They are, however, deficient in certain components. Thus, in comparison to platelets, microparticles had reduced contents of sialic acid (by 56.4%), galactosamine (by 48.2%), glucosamine (by 22.4%), galactose by (11.8%) and fucose (by 21.6%). Mannose content was increased by 11.8%. Total phospholipids in microplatelets were lower by 17.8%. Glycerophospholipids only were affected with phosphatidylserine being decreased as much as by 43.2%. Neutral glycosphingolipids, gangliosides and ceramides in microparticles were reduced by half. We conclude that the biochemical composition of microparticles probably reflects previous activation of progenitor platelets

19

The levels of glycosphingolipids, ceramides, sialic acid and glycogen are changed in plasma membranes of rat platelets harvested during recovery from immune-mediated thrombocytopenia

58%

Zdebska E., Soszynska B., Dobrowolski Z., Koscielak J.

Acta Biochimica Polonica

|

1996

|

tom 43

|

nr 3

Plasma membranes of rat platelets produced at normal platelet counts and during early recovery from immune-mediated thrombocytopenia were examined for the contents of carbohydrates, lipids and glycosphingolipids. Glucosylceramide, two monosialo-gangliosides and one disialo-ganglioside were found to be the major glycosphingolipids of platelets. During thrombocytopenia the contents of these glycosphingolipids as well as of ceramides were several fold elevated. Among carbohydrate constituents of platelets and platelet plasma membranes, glycogen content was increased and that of sialic acid decreased. These results are discussed in the light of literature data on relevant biochemical characteristics of megakaryocytes at different stages of maturation and on thrombopoiesis during acute experimental thrombocytopenia.

20

Helicobacter pylori infection and gastric secretion in duodenal and gastric ulcer patients - the effect of eradication after one year

58%

Laszewicz W., Zaremba-Woroniecka A., Gabryelewicz A.

Journal of Physiology and Pharmacology

|

1997

|

tom 48

|

nr 3

Ograniczanie wyników

Serum paraoxonase activity and total sialic acid in sheep with foot and mouth disease

The role of target membrane sialic acid residues in the fusion activity of the influenza virus: the effect of two types of ganglioside on the kinetics of membrane merging

The interaction between serotonin and n-acetylneuraminic acid or 2-keto-3-deoxyoctulosonic acid

Apoptosis of lymphocytes and sialic acid plasma level in patients undergoing cardiac surgery

The effect of carbohydrate moiety structure on the immunoregulatory activity of lactoferrin in vitro

Changes of the expression of Lewis blood group antigens in glycoproteins of renal cancer tissues

Elevated serum total sialic acid concentrations in sheep with peste des petits ruminants

How influenza's neuraminidase promotes virulence and creates localized lung mucosa immunodeficiency

Serum sialic acid, malondialdehyde, retinol, zinc, and copper concentrations in dairy cows with lameness

Bactericidal activity of normal bovine serum [NBS] directed against some Enterobacteriaceae with sialic acid-containing lipopolysaccharides [LPS] as a component of cell wall

Comparative study of sialidase activity and Gm3 content in B16 melanoma variants with different metastatic potential

Epitope dissection of receptor-active gangliosides with affinity for Helicobacter pylori and influenza virus

Purification and characterization of GlcNAc-6-P 2-epimerase from Escherichia coli K92

Lipopolysaccharide changes sialylation pattern in the mouse central nervous system

The role of complement activity in the sensitivity of Salmonella O48 strains with sialic acid-containing lipopolysaccharides to the bactericidal action of normal bovine serum

Similarity of vitamin D-binding protein (DBP) between various animal species in double immunodiffusion and western blotting

Purification and characterization of avian glycolipid: beta-galactosyltransferases (GalT-4 and GalT-3): cloning and expression of truncated betaGalT-4

In comparison to progenitor platelets, microparticles are deficient in GpIb, GpIb-derived carbohydrates, glycerophospholipids, glycosphingolipids, and ceramides

The levels of glycosphingolipids, ceramides, sialic acid and glycogen are changed in plasma membranes of rat platelets harvested during recovery from immune-mediated thrombocytopenia

Helicobacter pylori infection and gastric secretion in duodenal and gastric ulcer patients - the effect of eradication after one year