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1

AFLP marker polymorphism in cucumber [Cucumis sativus L.] near-isogenic lines different in terms of sex expression

100%
Witkowicz J., Urbanczyk-Wochniak E., Przybecki Z.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
2

AFLP marker polymorphism in cucumber [Cucumis sativus L.] near isogenic lines differing in sex expression

100%
Witkowicz J., Urbanczyk-Wochniak E., Przybecki Z.
Cellular and Molecular Biology Letters
|
2003
|
tom 08
|
nr 2
The AFLP technique was used to evaluate the level of polymorphism between two pairs of isogenic cucumber (Cucumis sativus L.) lines (NIL) differing in flower sex expression. The BSA techniques were also applied to find molecular markers linked to sex determination genes (dominant alleles) in those cucumber lines. Sex determination in cucumber is controlled by three main bands: F, M and Gy. The interaction of these bands is responsible for the formation of the various flower phenotypes with respect to sex in the analyzed lines: a female line 2gg with a ff/MM/gygy genotype, isogenic to a monoecious line B10 (genotype ff/MM/GyGy), and a female line Gy3 with a FF/MM/GyGy genotype, isogenic to a hermaphroditic line HGy3 (genotype FF/mm/GyGy). Using 56 combinations of AFLP primers, used for the analysis of lines 2gg and B10, gave 3794 bands, of which 155 (4.1%) were polymorphic. Ten bands distinguished gynoecious and monoecious bulks appearing at the same time in the appropriate parent; they are believed to be linked to the Gy locus. The isogenic lines Gy3 and HGy3 showed a higher level of polymorphism (14.2%). In this case, 55 combinations of primers gave 2996 reaction products, of which 430 showed variation. Twenty bands occurred in one bulk and in one parent, so they are probably associated with the M locus. Using the AFLP technique, the isogenicity of the lines was evaluated. The level of polymorphism (per pair of primer) between lines 2gg and B10 is 0.072% and is four times lower than that between the Gy3 and HGy3 lines (0.27%). The differences in the isogenicity of the lines can result from the degree of their relatedness, which may reflect the way they were derived.
3
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Variation in sex expression in Polish and Ukrainian populations of Taxus baccata L.

84%
Iszkulo G., Jasinska A. K.
Dendrobiology
|
2004
|
tom 52
29-32
The aim of the present study was to analyse the occurrence of monoecious individuals in selected populations of Taxus baccata in Poland and Ukraine. The investigation showed the monoecy in Taxus baccata to be extremely rare phenomenon. Only four cosexual individuals were found in three by seven populations examines and the frequency of monoecy was 0.13% on average among 2986 trees verified. Isolated seeds were found on individuals with large number of male flowers. Only one tree was found with numerous macro- and microstrobiles.
4

The identification of differential cDNA clones from two floral libraries in cucumber Cucumis sativus L.

84%
Kowalczyk M. E., Urbanczyk-Wochniak E., Przybecki Z.
Cellular and Molecular Biology Letters
|
2002
|
tom 07
|
nr Suppl.
5

Effect of ethephon [2-chlorethylphosphonic acid] on monoecious muskmelon [Cucumis melo L.] F1 hybrid seed production

84%
Korzeniewska A., Galecka T., Niemirowicz-Szczytt K.
Folia Horticulturae
|
1995
|
tom 07
|
nr 2
6

The isolation of cDNA clones from cucumber [Cucumis sativus L.] floral buds coming from plants differing in sex

67%
Przybecki Z., Kowalczyk M. E., Siedlecka E., Urbanczyk-Wochniak E., Malepszy S.
Cellular and Molecular Biology Letters
|
2003
|
tom 08
|
nr 2
In this study, we found flower cDNA clones which may be connected with the development of flower sex in cucumber. Two pairs of nearly-isogenic lines: gynoecious GY3 (FFMMGG) versus hermaphrodite HGY3 (FFmmGG) and monoecious B10 (ffMMGG) versus gynoecious 2gg (ffMMgg) were used for clone isolation. To obtain differentially-expressed clones, we applied the differential screening method. 454 clones from GY3 and 478 from B10 cDNA libraries were isolated. The results of RFLP analysis with 56 cDNA clones showed no clones which cosegregated with sex in cucumber. The 28 cDNA B10 and 33 cDNA GY3 clones isolated using the differential screening method were sequenced. Some of them seem to may play a role in cell differentiation or flower development. Among the 61 identified clones, 14 show high homology to plant proteins, although of unknown function. 11 show high homology to known proteins, and the possible function of some of them is discussed. For 3 clones, no significant similarity was found. The 31 clones displayed high homology to plant cDNA in EST database. The patterns of expression of five differential cDNA clones, 35GY3, 216GY3, 47GY3, 100B10 and 157B10, were analyzed in cucumber flower buds using in situ RT-PCR. The most interesting clone is 35GY3, because of its possible role in the inhibition of the development of male specific elements in the female cucumber flower.
7

The effects of methionine and cobalt ions on sex expression and development of Anemia phyllitidis gametophytes

67%
Kazmierczak A.
Acta Physiologiae Plantarum
|
2005
|
tom 27
|
nr 4A
One of the prime precursor for ethylene synthesis - L-methionine and the inhibitor of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) - Co2+- were tested for their effects on sex expression and development of Anemia phyllitidis fern gametophytes. Five concentrations of both chemicals (0, 10, 25, 50, 100 μM) were analysed with reference to antheridia and archegonia formation, number and size of cells as well as thalli length using the three-zone model of gametophyte structure. Both substances, however at different concentrations, enhanced the number of GA3-induced antheridia and similarly stimulated the cell number and inhibited thalli length. Both of them at 100 μM concentrations without GA3 induced meristematic area formation while methionine also induced archegonia in the apical parts of gametophytes. These findings correspond with the previous observations concerning the important role of ethylene synthesis precursor (ACC) in controlling gibberellic acid-induced male sex expression in ferns and broaden the knowledge about the mechanisms of fern gametophyte development.
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