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The work presented here demonstrates the utility of Caco-2 cells to detect enteroviruses in sewage. Viruses were concentrated by beef extract elution and organic flocculation prior to analysis by cell culture assays and RT-PCR. Enteroviruses were detected in all sewage samples, but only one sample was positive solely in RT-PCR assay. We proved that Caco-2 cells were more effective than RD and L20B cells in enterovirus isolation, depending on procedures used in the inoculation process.
The aim of the present paper was to evaluate the survival rate of Listeria monocytogenes in meat processing industry sewage, depending on the pH under laboratory conditions. In the present experiment we determined the time of survival of listeria bacilli in three kinds of sewage: crude, alkalized and acidified. The present research shows that Listeria monocytogenes survived longest in crude sewage where, as calculated with the use of regression analysis, theoretical time of survival was defined as 38 days, while in the alkalized sewage and acidified sewage the time was 33 and 25 days, respectively. Besides, the present experiment also investigated the potential of listeria bacilli survival in sewage under strongly acidic and alkaline conditions.
A total of 48 Pseudomonas spp., isolated from the Cukurova University Balcali Hospital sewage in Adana, were characterized on the basis of morphological, cultural and biochemical characteristics. To improve our understanding of the ecology of tellurite resistance in bacteria, the minimum inhibition concentrations (MIC) of potassium tellurite (K₂TeO₃) for growth were used to determine metal tolerance of the isolated strains. Most of the strains tolerated to 55 µgr/ml potassium tellurite. only strain Ps 37 tolerated relatively high concentrations of tellurite (80 µgr/ml). 27% of the strains possessed plasmid mediated tellurite resistance (Telr).
The objective of study was to define the removal rate of the indicator microorganisms on various types of trickling filters with regard to the influence of: magnetic stream and electric current passage, on the effectiveness of microorganisms adsorption onto the media. The hydraulic loading in the first eight variants were changing, variants 9-14 were peformed at a constant loading. The study results have indicated a positive influence of the extra energy sources i. e. a magnetic stream and electric current on the process of indicatory microorganisms removal from the waste water.
The presence of Rotavirus hominis and other rotaviruses in sewage and water is described. The problem of rotaviral world-wide infections causing severe or fatal disease of in­fants, children, adults and persons immu­no­com­pro­mised is discussed.
The work presented here demonstrates the utility of Caco-2 cells in the isolation of enteroviruses (EVs) from environmental and clinical materials. Thirty-two samples of cerebrospinal fluid positive in Pan-entero RT-PCR were taken for EV strain isolation in cell culture. Out of the 32 samples analysed, 22 (68.75%) were positive for enteroviruses by isolation in Caco-2 cells, and 10 (31.25%) were positive by isolation in RD cells. High viral titre in clinical specimens resulted in rate increase for isolation in Caco-2 cells and RD cells (87.5% and 50%, respectively). Also, the probability of isolation of enteroviruses from sewage in Caco-2 cells was 20 times higher that in RD cells. We proved that Caco-2 cells were more effective than RD cells in enterovirus isolation, irrespective of the material used in the inoculation process.
The objective of the study was to investigate pollution sources in the Vilnia River and evaluate the toxicity of sewage, contamination of the Vilnia River water and bottom sediments, using a complex of biological methods. Toxicity was assessed by use of various indices of test-organisms: seed germination and root growth in Lepidium sativum L., and mortality and physiological indices in early ontogenesis (embryos and larvae) of Oncorhynchus mykiss L. Changes in biological indices of Lepidium sativum L. and embryos and larvae of Oncorhynchus mykiss L. demonstrated that sewage of an identified point of the pollution source and the water of the Vilnia River 300 m downstream from the pollution source, bottom sediments of the Vilnia River nearby the pollution source and 300 m downstream from the pollution source could be defined as toxic.
Soil is a unique laboratory of transformations and energy flow, and in particular of biological sorption and synthesis of mineral components that can originate from various sources. The introduction of biomass or sewage into soil contributes to its nutrient enrichment. This can lead to periodical excess of these elements in soil and cause their migration from the soil system to underground waters, and eventually to open waters. Grass communities belong to a group of plants that make excellent use of nutrients present in soil and perform a very important role in additional purification of sewage after its mechanical purification. Grasses demonstrate resistance to the presence of large quantities of harmful compounds in sewage; they accumulate and neutralise them physiologically, thus preventing their dissemination. The aim of this paper was to evaluate content of some macro- and microelements as well as heavy metals in chosen grass species irrigated with purified sewage. In 1997- -2000, research was conducted near the Hajdów Sewage Works, using purified sewage for irrigation of grass communities. Three rates of irrigation (a – control without irrigation; b – irrigation in quantity 600 mm and c – irrigation in quantity 1200 mm) as well as two grass mixtures were tested. Content of basic macroelements (N, P, K, Ca, Mg), microelements (Cu, Zn, Mn) as well as heavy metals (Cd, Pb) in dominant grass species (Alopecurus pratensis, Phalaris arundinacea, Festuca arundinacea, Festuca pratensis and Phleum pratense) were determined. The content of the analyzed components in biomass was varied and depended on the grass species in the analyzing mixtures as well as on the applied rates of irrigation. Festuca pratensis and Festuca arundinacea were characterized by the largest capacity to take up calcium (Ca) and magnesium (Mg), Phalaris arundinacea – phosphorus (P) and zinc (Zn), while Alopecurus pratensis – cadmium (Cd) and lead (Pb). Biomass of Alopecurus pratensis was characterized by the lowest content of most of the elements, especially nitrogen, phosphorus, calcium and magnesium. The applied irrigation, especially the 1200 mm rate significantly increased potassium content and decreased manganese content in biomass of the analyzed grass species. The most useful grass species used to establish meadows irrigated with sewage are Phalaris arundinacea, Festuca arundinacea and Festuca pratensis.
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