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Competition is an evolutionary mechanism which exerts a selection pressure on living organisms. Forest trees compete for light, water and nutrients, especially at a young age. It was observed that the Quercus petraea and Padus serotina natural regenerations occupied the same site growing under the canopy of Scots pine (Pinus silvestris L.). To simulate the competition between young sessile oaks and black cherries found in forest, a controlled experiment was conducted using one-year-old seedlings of both species. There were eight treatments of different competition intensity. The treatments were established varying the number of potted seedlings and adding fresh cherry leaves to the substrate to enhance allelopathic effects. It was hypothesized that black cherry would reduce the height growth and diameter at root collar of sessile oak seedlings and this inhibitory effect would be magnified by an increasing number of cherry seedlings and/or fresh leaves. Black cherry as an invasive, fast-growing species was presumed to win the growth competition with oak. However, the differences in growth parameters would not only depend on genetic differences between the species, but also on the number of competing seedlings in pots and an allelopathic effect of cherry leaves. During the whole vegetative season, each two weeks, the growth parameters of seedlings (height, height increment and diameter at root collar) were measured. The results did not support the hypothesis that cherry had an inhibitory effect on oak growth, at least after one vegetative season. Contrary, a presence of cherry seedlings enhanced the oak height increment (F = 8.6, P <0.001) which might be due to either the strong interspecific competition for light or, less plausibly, positive allelopathic effect, or an interaction of both. Our results indicated a negative auto-allelopathic effect of cherry seedlings and/or fresh cherry leaves on height of cherry seedlings (F = 47.7, P <0.001). This invader showed a continuous and steep height increment within the whole vegetative season, whereas oak seedlings grew rapidly only in July. When compared the mean initial heights in April with those after the bud set in September, cherry was four fold and oak only two fold higher. A very intensive height increment gives black cherry an advantage over sessile oak at a young age which can disturb the spontaneous conversion of pine stands into a mixed pine-oak forest with a greater share of oak and other native deciduous tree species.
Assimilation and photosynthetic efficiency (maximal quantum yield) of young oaks were compared in coppice and standard sessile oak stands of comparable age (100 years) under different light intensity categories: under minimum light – ISF < 20%, low light – 20%30% during favourable and drought conditions. Measurements of maximal assimilation rates were performed at a constant temperature of the measurement block (20°C), a CO2 concentration of 400 μmol/l, flow 500 μmol/s and different light intensities: 0, 50, 250, 600, 1200 and 1800 μmol/m2s during three consecutive growing seasons (2012, 2013 and 2014). In every category at least 8 young naturally regenerated seedlings and sprouts of different coppice stools were measured. The quantum yield in optimal conditions in standards was highest in the category of closed canopy, while in coppices in medium light category. During severe drought in 2013 the drop in efficiency of standards was evident in all categories, while in coppices no differences in efficiency were observed between favourable 2012 and 2013 with expressed drought stress, proving the advantage of young coppices over standards in this particular light category. However, the beneficial effects of restoration coppicing are not guaranteed. It is our belief that in time such advantage might decrease; it would be therefore interesting to compare responses in time and define, when response abilities of both studied systems become equal.
Quercus robur and Q. petraea are important forest-forming species in Europe. Q. robur is believed to require more fertile soils, to be more tolerant to low temperatures and more sensitive to oak decline than Q. petraea. Thus chronologies of the two species from nearby localities were hypothesised to differ more strongly between species than between localities. Wood cores were collected on 23 research plots in 2 national parks and 12 forest districts. In each locality two plots (one with Q. robur and one with Q. petraea) were established in forest stands of similar age at similar site conditions wherever possible. Similarity between chronologies was assessed by the test of parallel agreement (Gleichlaufigkeit), Pearson correlation coefficients, and principal components. The plots were grouped by the Ward clustering method and according to 1st and 2nd principal components. The first 2 methods segregated chronologies nearly ideally into pairs of species from the same forest district, indicating that the chronologies differ between forest districts but not between species. Chronologies clustered in 2 large groups: northern and southern (on the basis of correlation) or northeastern and southwestern (on the basis of parallel agreement). Principal components also distinguished northeastern and southwestern chronologies, but less clearly. The results suggest that differences in climatic and soil requirements between the 2 oak species are generally too small in comparison with the differences caused by climatic factors.
In forest research and nursery practice there is often a need to monitor the condition and responses of trees to different stressors. Chlorophyll content in leaf is a good indicator of plant health and can be measured rapidly in many repetitions using the chlorophyll meter SPAD-502Plus. This practical tool provides the values of chlorophyll content in relative units (SPAD values), therefore it should be calibrated for each species to determine chlorophyll content in physiological units. In this study, the chlorophyll meter SPAD-502Plus was calibrated to be used for total chlorophyll (Chl), chlorophyll a (Chl a), chlorophyll b (Chl b) and carotenoids (Car) contents determination in leaves of Quercus petraea and Prunus serotina seedlings growing in different light environments. In the same leaf, SPAD values were measured with the Chl meter, and then photosynthetic pigments content (PP; chlorophyll and carotenoids) was consistently assessed using a conventional extraction method. The measurements were conducted once a month from May to November in three light treatments to obtain the widest possible range of the PP content values. To estimate total Chl content in leaves using the chlorophyll meter the quadratic polynomial functions: y = 0.0374x2 + 0.5345x + 0.5137 and y = 0.024x2 + 2.1998x – 32.7866 were obtained from the relationship between the Chl meter SPAD readings and total Chl determined spectrophotometrically for P. serotina and Q. petraea, respectively. Chl was higher under shade compared with full light regime and Car were linearly correlated with Chl. PP content was positively correlated with air temperature except for Car in P. serotina leaves. It was concluded that at the same soil conditions chlorophyll content in leaves of Q. petraea and P. serotina depended on species, light regimes and temperature of growth. The chlorophyll meter can be used as a practical tool to monitor and compare photosynthetic pigments content in leaves between tree species or populations acclimated to different environments together with a control of abiotic and biotic factors affecting pigments content and leaf optical properties.
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