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Occurrence of Leptospira sp. antibodies in swine in Poland

100%

Wasinski B.

Bulletin of the Veterinary Institute in Puławy

2007

tom 51

nr 2

The aim of the study was to evaluate the prevalence of antibodies specific to different serogroups of Leptospira sp. in swine populations in Poland. The serological survey was conducted in 2006. A total of 7 252 swine serum samples coming from all provinces of the country were investigated. The samples were examined by the use of a microscopic agglutination test. As antigens, six Leptospira serovars were used: Leptospira interrogans serovars - Icterohaemorrhagiae, Pomona, and Canicola, L. borgpetersenii serovars - Sejroe and Tarassovi, and L. kirschneri serovar Grippotyphosa. Altogether, 97 (1.34%) of the examined serum samples were found to be positive. They were obtained from 12 provinces. The highest number of samples among positive sera demonstrated titres to serovar Sejroe (44.33%) followed by Pomona (37.11%), Icterohaemorrhagiae (8.25%), Canicola (7.22%), and Tarassovi (3.09%). No samples with titres to serovar Grippotyphosa were found. The data concerning the highness titres to particular serovars and the prevalence of animals demonstrating antibodies to different serovars in particular provinces are presented and analysed in the article.

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Serological and molecular survey of Anaplasma phagocytophilum in Italian hunting dogs

80%

Ebani V. V., Bertelloni F., Turchi B., Cerri D.

Annals of Agricultural and Environmental Medicine

2013

tom 20

nr 2

The presence of Anaplasma phagocytophilum, a tick-transmitted zoonotic pathogen, has been reported in Italy in humans and several animal species, including dogs, but data concerning its prevalence in the Italian canine population are lacking. The aim of this study was to determine the prevalence of A. phagocytophilum infection in hunting dogs which live in geographic areas of central Italy where the infection had been previously detected in wild animals. Sera from 215 hunting dogs were tested by indirect immunofluorescence antibody test (IFAT) to detect antibodies to A. phagocytophilum. Buffy coat samples from the same 215 animals were submitted to a polymerase chain reaction (PCR) assay specific for the A. phagocytophilum 16S rRNA gene. Thirty-two (14.8%) dogs proved to be seropositive with antibody titres from 1:40–1:2,560. The highest seroprevalence was observed in dogs 6–10-year-old. Two seropositive (0.9%) animals were also PCR positive. Sequencing of PCR products revealed gene sequences of A. phagocytophilum in both cases. These results confirm the presence of A. phagocytophilum in the Italian areas studied; thus, the zoonotic potential of this agent should be considered particularly for people, as hunters, at risk of exposure to tick bites.

Ograniczanie wyników

1 Annals of Agricultural and Environmental Medicine

1 Bulletin of the Veterinary Institute in Puławy

1 Bertelloni F.

1 Cerri D.

1 Ebani V. V.

1 Turchi B.

1 Wasinski B.

1 2013

1 2007

Wyniki wyszukiwania

Occurrence of Leptospira sp. antibodies in swine in Poland

Serological and molecular survey of Anaplasma phagocytophilum in Italian hunting dogs