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Diagnostic value of different ELISA kits for the determination of antibodies against bovine leukemia virus [BLV]

100%

Kozaczynska B.

Bulletin of the Veterinary Institute in Puławy

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1999

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tom 43

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nr 2

The aim of these studies was to determine the performances of five commercial ELISA kits in the detection of BLV antibodies in serum samples. Different variants of ELI SA methods (indirect, blocking and double-well) were employed for these kits. Comparative investigations using 428 serum samples showed 418 consistent results (97.7%). Ten results (2.3%) proved to be inconsistent including 7 (1.6%) results which were totally different. The cause of these divergences in serological tests are discussed.

2

Diagnosis of bovine neosporosis: Recent advances and perspectives

86%

Ortega-Mora L. M., Fernandez-Garcia A., Gomez-Bautista M.

Acta Parasitologica

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2006

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tom 51

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nr 1

Neospora caninum is considered a major cause of abortion in cattle. Appropriate techniques for diagnosis of bovine neosporosis, both in vivo and in aborted foetuses, have been developed in the last ten years and some of them are commercially available. For diagnosis in live animals, detection of antibodies in serum or milk has been shown to be the best option both at the herd and the individual level. These techniques are excellent tools to examine N. caninum-associated abortion problems and to adopt some basic herd-control measures. Concerning foetal diagnosis, detection of compatible lesions by histological examination and parasites by PCR in brain (as well as heart and liver) are the best choices. Diagnostic criteria to distinguish foetal infection and Neospora-associated abortion are based not only on the demonstration of the parasite in the foetus but also on the extent and severity of the lesions in the foetus, foetal age and the assessment of neosporosis at the herd level. In the near future, new tools to diagnose infection should help to detect animals with parasite reactivation by testing the immune response to stage-specific antigens and lead to the development of molecular typing methods to characterise different parasite isolates. Finally, uniform diagnostic procedures need to be established between laboratories and countries in order to standardise result interpretation. The role of National or Regional Reference Laboratories is essential in countries or regions where control programmes for the disease are being developed.

3

Using combined recombinant protein in the diagnosis of bovine brucellosis

72%

Bulashev A., Jakubowski T., Mukantayev K., Tursunov K., Kiyan V., Zhumalin A.

Medycyna Weterynaryjna

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2018

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tom 74

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nr 03

The aim of this research was to obtain a combined recombinant protein consisting of immunodominant regions of Brucella outer membrane proteins (Omps) with the molecular weights of 25 kDa (Omp25) and 31 kDa (Omp31). The search for candidate proteins was carried out using NCBI PubMed and NCBI GenBank databases. The two most immunodominant regions of Omps were selected. The first region was in a position from 48 to 83 amino acids of Omp31, while the second one was in the position from 180 to 224 amino acids of Omp25. This combined sequence was designated as OmpBm-Ba. The pET32 vector was used for cloning and the expression of the combined recombinant protein in E. coli BL21(DE3). The antigenicity of recombinant OmpBm-Ba (rOmpBm-Ba) as compared with rOmp25 and rOmp31 has been tested on the sera of 109 cattle with positive results to brucellosis according to classical serological tests. The rOmpBm-Ba had a higher antigenicity than the single ones, since it confirmed the presence of Brucella-antibody in all serum samples with positive results of i-ELISA/rOmp25 and/or i-ELISA/rOmp31, as well as additionally revealing 7.3% and 31.2% seropositive animals, respectively. A comparative study of the diagnostic value of i-ELISA/rOmpBm-Ba and conventional tests on blood sera of 24 cattle subjected to a bacteriological examination at slaughter showed a higher reliability of enzyme immunoassay. Further research is necessary to get a more objective evaluation of the diagnostic accuracy of Brucella rOmpBm-Ba by challenging laboratory animals.

4

Evaluation of real-time PCR method for rapid diagnosis of brucellosis with different clinical manifestations

72%

Surucuoglu S., El S., Ural S., Gazi H., Kurutepe S., Taskiran P., Yurtsever S. G.

Polish Journal of Microbiology

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2009

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tom 58

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nr 1

15-19

In this study, we tested the advantages of TaqMan real time PCR technique and compare it to conventional methods using serum samples from patients with different clinical forms of brucellosis. A total of 50 patients were included in the study. Blood culture using BACTEC 9240 system, Standard Wright's tube agglutination, and real time PCR methods were used. Control blood samples from 30 people with no history of brucellosis or exposure to Brucella spp. were examined too. Serological assay was positive for 49 patients (98%). Forty-four (88%) of the 50 patients had a positive PCR result, whereas Brucella spp were isolated from blood cultures of 18 patients (36%). STA test was all positive for focal brucellosis. Real time PCR test was positive in 9 patients with focal disease (90%), whereas blood culture was positive only in 4 patients (40%). The sensitivity, specificity, positive and negative predictive values of the real time PCR method were calculated as 88%, 100%, 100%, and 83%, respectively. Our results suggest that the high sensitivity and specificity of real time PCR method make it a useful tool for diagnosis of brucellosis with different clinical manifestations.

5

Application of the ELISA for the virologic and serologic diagnosis of viral haemorrhagic disease of rabbits [VHD]

72%

Fitzner A., Niedbalski W.

Bulletin of the Veterinary Institute in Puławy

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1996

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tom 40

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nr 2

A new ELISA method for VHDV diagnosis (indirect sandwich ELISA) and Iiquid-phase blocking ELISA (LPBE) for the determination of antibodies to VHDV were developed. These assays are sensitive and specific and results may be directly correlated to those recorded by HA or HI. Besides, the presented techniques are rapid and relatively simple to perform and reagents are used economically. Therefore, the described immunoassays can be introduced to laboratory practice for the primary diagnosis of VHD.

6

Prevalence of antibodies against Chlamydophila psittaci and Chlamydophila abortus in cattle in Poland. A preliminary report

72%

Niemczuk K.

Bulletin of the Veterinary Institute in Puławy

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2005

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tom 49

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nr 3

7

Molecular and serological diagnosis of Borrellia burgdorferi infection among patients with diagnosed erythema migrans

72%

Kondrusik M., Grygorczuk S., Skotarczak B., Wodecka B., Rymaszewska A., Pancewicz S., Zajkowska J., Swierzbinska R., Hermanowska-Szpakowicz T.

Annals of Agricultural and Environmental Medicine

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2007

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tom 14

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nr 2

The aim of the study was to assess the frequency of Borrelia burgdorferi DNA detection in the blood and urine of patients diagnosed with erythema migrans, and compare the results of PCR-based methods with ELISA methodology. The latter was used to detect serum antibodies against Borrelia burgdorferi of the IgM and IgG classes, before and after antibiotic therapy. The study included 86 patients hospitalized in the Department of Infectious Diseases and Neuroinfections in the Medical Academy in Białystok, diagnosed with the erythema migrans phase of Lyme borreliosis. Examinations were carried out twice: the fi rst at the moment of diagnosis (Trial 1), the second after 4 weeks of antibiotic therapy. The study showed that antibiotic therapy in the early phase of borreliosis does not decrease the sensitivity of PCR and that after 4 weeks of therapy (Trial 2), spirochete DNA is still detectable in most patients (45/86). There was no correlation between detectability of spirochete DNA and the presence of antibodies against B. burgdorferi s.l. (assessed by ELISA) during the course of erythema migrans. The largest percentage of positive results in the detection of B. burgdorferi s.l. DNA was observed in patients who simultaneously possessed IgM and IgG antibodies against B. burgdorferi, while the lowest percentage of PCR positive results was among patients with only IgM antibodies.

8

Influence of serum or blood storage period on Hypoderma bovis antibodies detected by ELISA

58%

Cencek T., Boulard C.

Bulletin of the Veterinary Institute in Puławy

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2003

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tom 47

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nr 2

9

Requirements for haemagglutination inhibition test for diagnosis of parvovirus infections of carnivores

58%

Gorski J., Daniel A., Mizak B., Zwierzchowski J.

Bulletin of the Veterinary Institute in Puławy

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1993

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tom 37

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nr 2

Ograniczanie wyników

Diagnostic value of different ELISA kits for the determination of antibodies against bovine leukemia virus [BLV]

Diagnosis of bovine neosporosis: Recent advances and perspectives

Using combined recombinant protein in the diagnosis of bovine brucellosis

Evaluation of real-time PCR method for rapid diagnosis of brucellosis with different clinical manifestations

Application of the ELISA for the virologic and serologic diagnosis of viral haemorrhagic disease of rabbits [VHD]

Prevalence of antibodies against Chlamydophila psittaci and Chlamydophila abortus in cattle in Poland. A preliminary report

Molecular and serological diagnosis of Borrellia burgdorferi infection among patients with diagnosed erythema migrans

Influence of serum or blood storage period on Hypoderma bovis antibodies detected by ELISA

Requirements for haemagglutination inhibition test for diagnosis of parvovirus infections of carnivores