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The Golgi technique stain was used to reveal the cellular structure of the neostriatum (nucleus caudatus and putamen) in the guinea pig. The computerised reconstructions were made from Golgi impregnated neurones. On the basis of various criteria, 4 types of neurones were distinguished in the guinea pig neostriatum: 1. The rounded neurones (most numerous) with 5–8 thin dendritic trunks; 2. The triangular nerve cells with 3 thick dendritic trunks; 3. Two types of multipolar neurones differing in dendritic arborisation pattern with 4–6 and 7–9 primary dendrites, respectively. 4. The pear-shaped cells, which divide into two distinctly different subpopulations.
Variability of the bony structures located in the maxillary sinus, and of the lateral nasal wall topography, have practical significance during surgical procedures conducted by maxillofacial surgeons or otolaryngologists. The retrospective analysis of 111 computed tomography examinations of patients (52 male and 59 female) diagnosed in our institution was made to evaluate anatomical variations of the maxillary sinus. In the study the frequency of the Haller cell was 29/222 (13%), and the prevalence of one or more septa per sinus was 49/222 (26%). The infraorbital recess was found in 6/222 (3%) of cases. The mean width of the nasal duct was enlarged at the side where the Haller cell was present (p < 0.01) or where bony septa were absent in the maxillary sinus (p < 0.01). Bony structures of the maxillary sinus and changes in topography of the lateral nasal wall should compel surgeons to carefully analyze the computed tomography scans before operations in this area. (Folia Morphol 2009; 68, 4: 260–264)
This study shows that an ICP4– replication-deficient herpes simplex virus containing the Moloney murine leukaemia virus LTR fused with the coding sequence for the β-galactosidase gene can be used as a very effective vector for delivering the β-galactosidase reporter gene into the rat brain septum. F344 rats received bilateral stereotaxic injections into the nucleus of the diagonal band and into the medial septum. The X-gal stain was used to detect the activity of the expressed β-galactosidase enzyme. The delivered reporter gene was expressed successfully not only in the neuronal cells of the injected areas but also in cells that project to the injection area such as cortex cells about 6mm away from the injection sites. Expression was visible at 1, 3 and 9 weeks following injection. We conclude that this vector can effectively deliver genes into different regions of the mature mammalian brain and also to areas distant from the injection site.
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