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Ejaculates from three rams were collected with artificial vagina at one day (OD1) and four day intervals (FDsI). Part of the semen samples were pooled, diluted with skim milk based extender: -egg yolk (10%) -glycerol (5%), loaded into 0.25 ml straws, equilibrated, frozen, and afterwards stored in liquid nitrogen. The following spermatozoon parameters were determined in fresh and frozen-thawed semen: motility, viability, abnormality, abnormal acrosome, and membrane integrity. Semen collected at ODI had significantly higher freeze-ability compared with semen collected at FDsI for spermatozoon motility. Frozen-thawed semen collected at ODI had higher viability and membrane integrity, and lower abnormality and abnormal acrosome percentages compared with FDsI. It was concluded that in order to use cryopreservation of the Tushin ram semen, ODI instead of FDsI collection should be preferred.
The objective of the present research was to determine the effect of different quantities of vitamin C supplied and of its subsequent withdrawal on selected physical and chemical characteristics of ejaculate collected from boars of the Duroc and Hampshire breeds, as well as Duroc × Hampshire (D × H) and Hampshire × Duroc (H × D) crossbreds. The control group (C) consisted of 4 Durocs, 4 Hampshires, 4 Duroc × Hampshire crossbreds and 4 Hampshire × Duroc crossbreds. The same animals made up the experimental groups: E1, E2, and E3. The boars in groups E1 and E2 were consecutively supplied with vitamin C in the spring season, as follows: 2.4 g/100 kg b.w./day was administered to E1 for 30 days, and then 1.3 g/100 kg b.w./day was given to E2 for another 30 days. In group E3, vitamin C supplementation was ceased. Ejaculates were evaluated to measure the following traits: ejaculate volume, sperm concentration, sperm count in total ejaculate and in a dose of ejaculate, progressive motility, and the number of doses. A significant positive effect of vitamin C administered in amounts of 2.4 g and 1.3 g on ejaculate volume was observed in Durocs and D × H hybrids. With respect to Hampshires and D × H hybrids, statistically significant differences (at P ≤ 0.01) were observed between groups C and E3. The concentration of sperm cells in 1 ml of ejaculate was higher in samples from the experimental groups compared with samples from the control group, and this relationship depended on the amount of vitamin C administered and on the discontinuation of administration. A significant (P ≤ 0.01) increase in sperm count in total ejaculate was noted in Durocs, D × H crossbreds and H × D crossbreds in groups E1 and E3 and in Hampshires in E1, E2 and E3. A significant improvement in the progressive motility of sperm was noted in Hampshires in groups E1, E2, and E3. Fourteen days after vitamin C withdrawal in group E3, the highest pH value and alkaline phosphatase (ALP) activity were observed.
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