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The influence of selenium compounds of different structure on morphology, blood biochemistry and phagocytic capability of granulocytes in rats

100%

Musik I., Kielczykowska M., Donica H.

Roczniki Państwowego Zakładu Higieny

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2013

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tom 64

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nr 2

Background. Selenium belongs to important microelements. Numerous studies have revealed relationships between its deficiency and occurrence of diverse illnesses, but the question of the proper form and dose of Se-supplementation still remains unsolved. Objective. In the present study the influence of different selenium compounds on blood morphology and biochemistry as well as on phagocytic capacity of granulocytes and NBT test in rats was investigated. Material and methods. Adolescent male Wistar rats were divided into four groups (ten animals each): I – control, received saline; II – received sodium selenite Na2SeO3; III – received selenoorganic compound A of chain structure 4-(o-tolyl-)-selenosemicarbazide of 2-chlorobenzoic acid; IV – received selenoorganic compound B of cyclic structure 3-(2-chlorobenzoylamino-)- 2-(o-tolylimino-)-4-methyl-4-selenazoline. The administration was performed by stomach tube at a dose of 5 · 10-4 mg Se g-1 b.w. once a day for 10 days. Results. Selenium compounds treatment decreased haematocrit. Erythrocytes number was unchanged in all groups receiving Se vs. control, whereas leucocytes number was depressed in groups II and IV. Haemoglobin was significantly decreased in group III. White blood count was altered in groups II and III, where all parameters were markedly decreased except for lymphocytes in group III and remained unchanged in group IV. The outcomes regarding selenium effect on biochemistry parameters of blood showed that urea remained unchanged, glucose was statistically decreased in groups II and III, whereas cholesterol was significantly diminished in group II and increased in group III vs. control. Results concerning phagocytosis and NBT test displayed that % of positive cells were decreased in groups II and III, whereas remained unaltered in group IV vs. control. Conclusions. As cyclic selenoorganic compound B did not cause many significant changes of the studied parameters it may be suggested that after further researches it could be taken into account as a possible selenium supplement.

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The effects on lead absorption and toxicity of the preincubation of Allium sativum L. roots in selenium compounds

100%

Glinska S., Jasinska M., Gabara B.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

3

Utilization of selenium compounds in nutrition of lambs

84%

Slupczynska M., Kinal S., Hadrys M., Krol B.

Journal of Elementology

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2009

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tom 14

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nr 1

157-163

The level of mineral utilization depends on many factors related to animals as well as to the chemical form of given nutrients. It has been experimentally demonstrated that animals utilize inorganic forms of minerals less efficiently than organic ones. Hence, an attempt has been made to evaluate the bioavailability of selenium bound in different compounds to lambs. Selenium supplementation in fodder mixtures was another aspect included in our tests. Thus, an experiment was conducted on 48 growing lambs, testing the level and chemical forms of selenium in fodder mixtures for animals. In group I (the control) lambs received fodder mixture without any selenium supplement. In the experimental groups, selenium was supplemented as sodium selenite in the amount 0.2 mg Se kg-1 d.m. feeds (II), or selenium enriched yeast (Se-yeast) in the amounts of 0.1 and 0.2 mg Se kg-1 d.m. of feed groups III and IV, respectively. At the end of the experiment, when animals had reached 35 kilos of weight, blood samples were taken. The activity of glutathione peroxidase was estimated in heparinized blood samples. Eight lambs were chosen from each group and killed to collect samples of soft tissues (liver, kidney, brain, muscle). The content of selenium was determined in the tissue samples. Supplementation of feeds for lambs with selenium, both inorganic (sodium selenite) and organic (Se-yeast), increased the content of the element in soft tissues of animals. The highest level of the element was found in the liver and kidneys: 4.65 and 4.90 and 2.10 and 2.30 mg kg-1 fresh tissue, of the lambs receiving sodium selenine and Se-yeast in the amount 0.2 mg Se kg-1 D.M. of feed (groups II and IV), respectively. Selenium compounds added to feeds given to lambs significantly (P£0.01) increased activity of GSH-Px in blood, especially in the case of animals which received yeast enriched with selenium. In blood of the lambs which received feeds with Se-yeast in the amount 0.2 mg Se kg-1 d.m. of feed (group IV), the activity of glutathione peroxidase was by 129.71 U gHb-1 higher, and of the animals which received Se-yeast in the amount 0.1 mg kg-1 d.m. or sodium selenite, the activity of the enzyme was higher by 86.33 and 86.35 U gHb-1, respectively, than the activity of this enzyme in blood of animals from the control group. Supplementation of lambs’ rations with Se in the form of selenite or yeast enriched with selenium forms increased the content of Se in soft tissues and gluthatione peroxidase activity in comparison with animals which did not receive additional doses of this nutrient in fodder mixtures. The availability of Se was more profoundly affected by the amount of the element added rather than its form.

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The influence of selenium compounds on the permeability of wheat callus cell plasma membranes in the presence of cadmium ions

84%

Wrona M., Sieprawska A., Tejchman W., Filek M.

Acta Physiologiae Plantarum

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2007

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tom 29

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nr 1 Suppl.

5

Influence of dietary CLA isomers and selenium compounds on the fatty acid and amino acid profiles in blood plasma of rats

84%

Niedzwiedzka K. M., Korniluk K., Kowalczyk J., Czauderna M.

Journal of Animal and Feed Sciences

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2007

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tom 16

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nr 2

257-273

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Influence of the administration of selenium compounds on tissue magnesium concentration in rats

84%

Musik I., Kielczykowska M., Hordyjewska A., Pasternak K.

Journal of Elementology

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2010

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tom 15

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nr 3

539-547

Magnesium and selenium belong to important bioelements. Magnesium is the second most abundant intracellular macroelement, which takes part in the metabolism of carbohydrates, nucleic acids, protein and lipids. Selenium is an essential microelement, whose deficit has been stated in many different pathological states. Much research on safe and effective selenium supplementation has been performed for the last fifty years but the results still remain unsatisfactory. The aim of our study was to investigate the influence of inorganic sodium selenite Na2SeO3 and two selenoorganic compounds synthetized at our chair on magnesium concentration in tissues of adolescent male Wistar rats. Inorganic selenite was administered as a water solution, whereas organic compounds: 4-(o-tolilo)-selenosemikarbazyd of 2-chlorobenzoic acid of a chain structure (ORG-C) and 3-(o-chlorobenzoylamino)-2-(o-tolylimino)-4- -methyl-4-selenazoline of a ring structure (ORG-R) were suspended in emulsion (oil, arabic gum and water). Selenium compounds were given to rats at a dose of 5⋅10–4 mg Se g–1 b.w. once a day for a period of 10 days. The control group was treated with saline. The administration was performed with use of a stomach tube. In comparison to the control group, selenium supplementation caused decrease in magnesium concentration in kidney and lung tissues, but did not cause any changes in the brain and heart muscle. In the liver and spleen it was only ring selenazoline that affected magnesium concentration, increasing it in the liver and decreasing in the spleen. In the femoral muscle it was only the selenosemicarbazide chain that exerted the significant effect causing a decrease in Mg concentration vs the control group. Selenium supplementation influences the tissue magnesium concentrations depending on tissue and structure of the supplement. Irrespective of the administered compound, it lowered magnesium in kidneys and lungs but caused no changes in the brain and heart muscle. In the liver, spleen and femoral muscle, alterations in the magnesium concentration were dependent on the provided supplement.

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Influence of selenium compounds on glutathione peroxidase [GSH-Px] level in lamb blood

84%

Hadrys M., Kinal S., Antonowicz-Juchniewicz J., Jedrychowska I.

Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine

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2007

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tom 10

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nr 1

8

Zaleznosc pomiedzy akumulacja cynku w siewkach Zea mays L. a rodzajem i stezeniem zwiazku selenu zastosowanego w uprawie

67%

Kita A., Pazurkiewicz-Kocot K.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2006

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tom 509

231-238

Jednym z ważniejszych czynników warunkujących pobieranie, akumulację oraz dystrybucję pierwiastków w tkankach roślinnych jest interakcja pomiędzy jonami poszczególnych pierwiastków. W pracy badano zależność pomiędzy akumulacją Zn w korzeniach, mezokotylach oraz liściach Zea mays L. oraz formą i koncentracją selenu. Doświadczenia zostały przeprowadzone na 8-9 dniowych siewkach Zea mays L. var. K33xF2 wyhodowanych na płynnej pożywce Hoaglan- da (pH = 6,5) w temperaturze około 25°C, zawierającą w swoim składzie dodatkowo związki Na₂SeO₄, NaHSeO₃, SeO₂, Na₂Se o stężeniu 10⁻⁶-10⁻³ mol·dm⁻³. Akumulację cynku w liściach, mezokotylach oraz korzeniach mierzono metodą emisyjnej spektrometrii atomowej (ICP-AES). Zaprezentowane w pracy wyniki wskazują na zależność pomiędzy pobieraniem, dystrybucją i akumulacją cynku w roślinach kukurydzy, a obecnością selenu w środowisku zewnętrznym rosnących roślin, jego formą chemiczną i stężeniem.

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Influence of selenium on lead absorption and localization in meristematic cells of Allium sativum L. and Pisum sativum L. roots

67%

Glinska S., Gabara B.

Acta Biologica Cracoviensia. Series Botanica

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2002

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tom 44

The effect of different concentrations (80-640 µM) of sodium selenate and sodium selenite on lead absorption by Allium sativum (selenium absorber) and Pisum sativum (selenium nonaccumulator) roots treated with 10 µM Pb(NO3)2 was investigated. Lead alone and with either of the selenium compounds was supplied in aqueous solutions for 24 h. Selenite diminished lead content in the two plant species’ roots more effectively than selenate. The rhodizonate method showed the absence of lead in the presence of only 160 µM sodium selenite and only 640 µM sodium selenate. The effects of selenium compounds at 80 µM concentration on lead localization in meristematic cells were also investigated. While selenite in garlic cells reduced the number of electron-dense deposits in the cell wall and diminished their size in vacuoles, it increased their number in cytoplasm and plastids, and enlarged them in mitochondria. In pea cells it caused the disappearance of electron-dense sediments from the Golgi apparatus, endoplasmic reticulum, vacuoles and nucleus, or reduced their number in mitochondria, cytoplasm and plastids (lack of large deposits). On the basis of literature data we assume that selenium reduces the lead concentration in Allium sativum and Pisum sativum roots due to the formation of Pb-Se complex in the incubation medium.

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Effect of organic selenium compounds on the activity of glutathione peroxidase and superoxide dismutase in selected mouse tissues

67%

Musik I., Staroslawska E., Pasternak K.

Bulletin of the Veterinary Institute in Puławy

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2003

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tom 47

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nr 2

Ograniczanie wyników

The influence of selenium compounds of different structure on morphology, blood biochemistry and phagocytic capability of granulocytes in rats

The effects on lead absorption and toxicity of the preincubation of Allium sativum L. roots in selenium compounds

Utilization of selenium compounds in nutrition of lambs

The influence of selenium compounds on the permeability of wheat callus cell plasma membranes in the presence of cadmium ions

Influence of dietary CLA isomers and selenium compounds on the fatty acid and amino acid profiles in blood plasma of rats

Influence of the administration of selenium compounds on tissue magnesium concentration in rats

Influence of selenium compounds on glutathione peroxidase [GSH-Px] level in lamb blood

Zaleznosc pomiedzy akumulacja cynku w siewkach Zea mays L. a rodzajem i stezeniem zwiazku selenu zastosowanego w uprawie

Influence of selenium on lead absorption and localization in meristematic cells of Allium sativum L. and Pisum sativum L. roots

Effect of organic selenium compounds on the activity of glutathione peroxidase and superoxide dismutase in selected mouse tissues