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In the present investigation the seeds of Pigeon pea (Cajanus cajan (L.) Millsp) were treated with different doses of gamma radiation (05KR, 10KR, 15KR, 20KR, 25KR, 30KR, 35KR, 40KR, 45KR, and 50KR) and concentration of Ethyl Methane Sulphonate (05mM, 10mM, 15mM, 20mM, 25mM, 30mM, 35mM, 40mM, 45mM, and 50mM) for studying seed germination, seedling height, (shoot and root), seedling injury, seedling vigour index, and seedling survival of plants at 30th day. The seed germination percentage was decreased with increased in the concentration/doses when compared to control. The LD50 (Lethal dose) value was determined based upon the seed germination percentage. The 50 percentage of seed germination and reduction was observed in 20KR of gamma rays and 25mM of EMS and it is considered as LD50 value for both the treatments. The decrease in seed germination was more prominent with gamma rays than that of EMS treatments. The seedling parameters of gamma rays and EMS treated seedlings were progressively decreased with increase dose/concentration in all mutagenic treatments when compared to control. The maximum seedling parameters were observed in 05KR of gamma rays and 05mM of EMS. Minimum seedling parameters were observed in 50mM of EMS and 50KR of gamma rays respectively.
The paper presents the results of laboratory research on the effects of different soil compaction caused by varying unit pressure and its impact on germination and growth parameters of common beech. Soil for the research was taken from the forest stand, and then poured into prepared PVC cylinders. Pressure was exerted on the soil with use of the stamp, on a specially prepared static stand. Unit pressure ranged from 50 to 300 kPa with 50 kPa resolution. Each variant of the exerted unit pressure and control without pressure was repeated three times. 21 seeds of common beach were sown into a single cylinder. The cylinders were then placed in controlled conditions and after 2.5 months all seedlings parameters were measured. 234 seedlings were analysed (sown 441). It was found that the seeds were influenced by the place of sowing. There were significant correlations between the exerted unit pressure and the length of root system of plants sown away from direct exposure to the stamp, as well as all the plants together. With the increased unit pressure the length of root system decreased. Sowing place turned out to be important for the root neck diameter, root dry weight and whole plants.
Mechanical site preparation (MSP) is commonly used to enable forest regeneration of clear-cuts. Less intense methods may be more suitable from an environmental point of view, but such a method can result in the lack of natural regeneration. We compare the influence of three MSP methods on natural regeneration of Scots pine. The methods differ in their degree of soil disturbance. The effects of MSP by forest plough (FP), active plough (AP), and forest cutter (FC) on density, quality, and biometric parameters of one-year old seedlings were determined. The highest density of seedlings was obtained in the FP treatment (188,000 seedlings ha–1). The seedling density was significantly lower in the AP method (121,000 seedlings ha–1), and in the FC variant (36,000 seedlings ha–1). The best quality seedlings were found in the FC variant, and the worst – in the FP treatment. Most biometric parameters of seedlings did not differ by MSP method, except for the root length and root:shoot ratio. By the end of growing season, in the FP treatment, 1-year old seedlings formed a top bud more than twice as often as the seedlings from the AP and FC treatments. The FP method led to the best Scots pine natural regeneration. The results obtained in the FC variant were not as good as in the FP method, though they were still acceptable from the silvicultural point of view. The FC method was the least intensive method of site preparation, yet it is considered the most appropriate from the environmental (soil protection) point of view.
Lactic acid has been known as one of compounds to cause cellular harm in waterlogged tissues through the process of cytoplasmic acidosis. The effects of lactic acid on α-amylase activity and phytic acid (myo-inositol 1,2,3,4,5,6-hexaphosphate) content using an assay for high phosphate in the germination stage of rice were evaluated. It is showed that lactic acid inhibited rice germination at every treated dose. The reduction of α-amylase content attributed to lactic acid at 24 h after germination of rice seeds was observed. The findings highlighted the effects of lactic acid on α-amylase activity and phytic acid content and suggested that this compound may play a potent role as a germination regulator in rice.
Seedlings of sorghum varieties (M35-1, a drought tolerant species; SPV-839, a drought sensitive one) differing in their drought tolerance were subjected to 150 mM NaCl stress for a short duration of time (up to 72 h). Both the varieties failed to exhibit efficient ion exclusion mechanism like that of salt tolerant species, but in turn resulted in higher accumuiation of Na+ and Cl- ions over a period of 72 h salt stress. In addition, accumulation of calcium, potassium and proline in seedlings of sorghum varieties was moderate to short-term NaCl stress. The modulation of antioxidant components significantly diverged between the two varieties during seed germination, further the efficiency of antioxidant scavenging system is maintained during short-term NaCl treatments. In compari son to tolerant variety, the sensitive variety depicted higher SOD activity under control and salinity treatments but specific activity of catalase was significantly reduced. In contrast, drought tolerant variety exhibited efficient hydrogen peroxide scavenging mechanisms with higher catalase and GST activities under control and salt stress conditions, but not in the sensitive one. In conclusion, our comparative studies indicate that drought tolerant and susceptible varieties of sorghum induce efficient differential oxidative components of enzymatic machinery for scavenging ROS thereby alleviating the oxidative stress generated by salt stress during seedling growth.
Research in our laboratory has focused on the analysis of the functions of a variety of enzymes that are involved in the scavenging of reactive oxygen intermediates (ROI) such as superoxide radicals (·O⁻₂ ) and hydrogen peroxide (H₂O₂). Recent work has been on transgenic plants that over-express glutathione S-transferases (GST) that also have glutathione peroxidase activity. Transgenic tobacco plants that contain gene constructs that encode two different tobacco GST’s had elevated levels of both GST and GPX activity. Analysis of mature vegetative transgenic tobacco plants that over-express GST/GPX failed to show any increase in paraquat tolerance or protection from photooxidative stress. However, seeds of these GST/GPX-expressing tobacco lines are capable of more rapid germination and seedling growth at low temperatures and at elevated salt concentrations. Reduced levels of lipid peroxidation were noted in GST/GPX-expressing seedling compared to control seedlings under both stressful and non-stressful conditions. In addition, GST/GPX-expressing seedlings significantly accumulated more oxidized glutathione (GSSG) than control seedlings during stress. These characteristics clearly indicate that over-expression of GST/GPX in transgenic seedlings can have substantial effects on their stress tolerance. Furthermore, it appears that this effect is due primarily to the elevated levels of GPX activity.
Quercus robur seedlings are very often infested by oak powdery mildew caused by Erysiphe alphitoides. This disease attacks primary leaves with high insolation. In our experiment seedling growth in different light conditions was observed. Seedlings were derived from intact seeds or from seeds where the distal ends of acorns were cut off. Acorns were sown in pots at four light conditions (2%, 8% and 30% light transmittance and in full sunlight). For half of the seedlings we reduced the influence of powdery mildew by using a fungicide. Height of seedlings, shoot dry mass and the degree of damage caused by Erysiphe alphitoides were measured. The highest damages were in full light conditions and the lowest in the 30% light treatment. The influence of acorn reduction on powdery mildew infection was not significant (p=0.0763), however, in the full light conditions the seedlings from cut acorns were ca. 30% less damaged. The cutting of acorns also alters the height of seedlings and shoot mass. The tallest seedlings were from cut seeds growing in the 30% light treatment. The influence of the fungicide was lowest in the 30% light treatment where the decrease in damage was only ca. 10%. For nursery practice we can say that oak seedling production from cut off acorns under 30% light transmittance should give the best results of seedling growth even without the fungicide application.
Competition is an evolutionary mechanism which exerts a selection pressure on living organisms. Forest trees compete for light, water and nutrients, especially at a young age. It was observed that the Quercus petraea and Padus serotina natural regenerations occupied the same site growing under the canopy of Scots pine (Pinus silvestris L.). To simulate the competition between young sessile oaks and black cherries found in forest, a controlled experiment was conducted using one-year-old seedlings of both species. There were eight treatments of different competition intensity. The treatments were established varying the number of potted seedlings and adding fresh cherry leaves to the substrate to enhance allelopathic effects. It was hypothesized that black cherry would reduce the height growth and diameter at root collar of sessile oak seedlings and this inhibitory effect would be magnified by an increasing number of cherry seedlings and/or fresh leaves. Black cherry as an invasive, fast-growing species was presumed to win the growth competition with oak. However, the differences in growth parameters would not only depend on genetic differences between the species, but also on the number of competing seedlings in pots and an allelopathic effect of cherry leaves. During the whole vegetative season, each two weeks, the growth parameters of seedlings (height, height increment and diameter at root collar) were measured. The results did not support the hypothesis that cherry had an inhibitory effect on oak growth, at least after one vegetative season. Contrary, a presence of cherry seedlings enhanced the oak height increment (F = 8.6, P <0.001) which might be due to either the strong interspecific competition for light or, less plausibly, positive allelopathic effect, or an interaction of both. Our results indicated a negative auto-allelopathic effect of cherry seedlings and/or fresh cherry leaves on height of cherry seedlings (F = 47.7, P <0.001). This invader showed a continuous and steep height increment within the whole vegetative season, whereas oak seedlings grew rapidly only in July. When compared the mean initial heights in April with those after the bud set in September, cherry was four fold and oak only two fold higher. A very intensive height increment gives black cherry an advantage over sessile oak at a young age which can disturb the spontaneous conversion of pine stands into a mixed pine-oak forest with a greater share of oak and other native deciduous tree species.
To compare the effect of NaCl, CaCl2 and their combinations on germination and early seedling growth stages of Lycopersicum esculentum L., were studied under pot experiments 2008. Results indicated that significant increases were recorded in percentage of germination, seedling fresh and dry weights, seedling length, water content, catalase activity and photosynthetic pigments (chlorophyll a, b and total chlorophylls as well as carotenoids) under the low level concentration (20 mM) of NaCl or CaCl and their combination (1:1). On other hand increasing salt concentration in nutrient solution caused significant decrease in all of these parameters. The great reduction occurred under high salinity level of NaCl (50 mM). Meanwhile, peroxidase activity increased significantly with increasing salinity levels from 20 mM to 50 mM of both applied salinity types. Besides, peroxidase activity under NaCl salinity showed a marked increase followed by NaCl + CaCl2 (1:1) and CaCl2 at 50 mM.
W warunkach laboratoryjnych określono wpływ zasolenia na wzrost i foto-syntezę dwóch odmian jęczmienia jarego Promyk i Suweren. Badanie prowadzono w kulturach wodnych przy użyciu pożywki Hoaglanda z różnymi stężeniami chlorku sodu (NaCl): 0 – kontrola, 50, 100, 150 mmol·dm-3. Po 28 dniach wzrostu wykonano pomiary biometryczne i wymiany gazowej. Uzyskane wyniki wykazały, że wraz ze wzrostem stężenia chlorku sodu w pożywce następowało istotne hamowanie wzrostu liści, a także średniej wartości świeżej i suchej masy części nad-ziemnej i korzeni. Stwierdzono również obniżenie natężenia asymilacji CO₂ wraz ze wzrostem stężenia chlorku sodu. Stymulujący wpływ NaCl na długość korzeni zaobserwowano przy stężeniu 50 mmol·dm-3.
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