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Seeds of Pyrus calleryana were sampled from 11 stations located in Poznań green areas. Three of them represented cultivar ‘Capital’, whereas the remaining eight represented cultivar ‘Chanticleer’. The viability of seeds was qualified by means of tetrazolium test. The results of staining test showed that viability of Callery pear seeds was high and ranged from 90 to 100%.
Liparis loeselii (L.) Rich. is an endangered orchid species which occurs in wetland habitats. For successful conservation of this orchid it is necessary to recognize its adaptive traits and special habitat requirements. Therefore, we examined morphological and anatomical traits in relation to the water level, cover of vascular plants and seed viability of L. loeselii in habitats with different water level fluctuations. Field studies of L. loeselii were carried out in nine localities and four habitat types: alkaline fen, calcareous fen with Cladium mariscus, transition mire and quaking bog. The fluctuations of seasonal water level were defined in the range from dry substrate to short time flooding (up to two weeks). Leaf length and width of 30 flowering specimens of L. loeselii as well as angle between the leaf blade and the axis of the inflorescence were measured at each locality. Seed viability and root anatomy were examined in the laboratory. The leaf angle was significantly affected (P < 0.05) by water level in the studied habitat. The leaf length to width ratio in each population of L. loeselii did not show significant differences between the years, but was characteristic for the habitat type. L. loeselii sensitivity to the long-term flooding in the habitat is associated with a lack of aerenchyma in its roots. During short-term flooding specimens of L. loeselii can survive due to a shallow root system and hyponastic leaf movements. As evidenced by seed viability, the most favourable growth conditions for L. loeselii are in the habitats with partially water saturated substrate with balanced water supply.
The objective of this research was to determine the suitability of boiling water test in the prediction of seed viability in leek seed and to compare this test with standard germination and vigour test. Basic principle of boiling water test is the evaluation of radicle protrusion from the seeds immersed to boiling water for certain time. The viability of aged and unaged leek seeds in boiling water test was correlated with standard germination and vigour test. In this research, the mean germination of unaged leek seeds varied from 29,63% to 70,18% depending on the cultivars. While the range of boiling water test in unaged leek seeds was between 35,57% to 51,06%, the range for emergence was between 27,63% to 72,05%. The viability of aged and unaged leek seeds in boiling water test was correlated with standard germination test. According to the results of this research, it might be assessed that boiling water test is a rapid and simple method to measure seed viability by evaluating radicle protrusion of leek seeds in boiling water. In conclusion, boiling water test as a new viability test for leek seed could be used as a rapid method for determining the viability in leek seeds.
Fruits of large-leaved lime dried to 10% may be stored for 16 years in sealed containers at –3°C without loosing seed viability. Dormancy of seeds, extracted from hard fruit coats, may be released after chemical scarification in concentrated sulphuric acid for 10 minutes, followed-by stratification without any medium (chilling) at the temperature of 3°C, for 20–24 weeks, i.e. until the first seeds start to germinate. After such pretreatment, during the germination test conducted at alternating temperatures 3~15°C (16 + 8 hours/day) seeds germinate near 90% in several weeks. For seedling production scarified and stratified seeds should be sown in early spring into trays under a plastic tunnel which ensures a high percentage of seedlings emergence. Sowing of the pretreated seeds in spring in a open nursery gives poor results.
Xanthium sibiricum, an annual weed, unexpectedly and dramatically occupied the exposed drawdown area after water had been impounded for the first time in the newly created Three Gorges Reservoir in China. In order to explain this phenomenon and establish an appropriate management strategy, the effects of constant submersion on seed viability and germination of X. sibiricum were investigated at two constant temperature regimes (25oC and 30oC) under artificial laboratory conditions. The results indicated that the seeds of X. sibiricum exhibited a high level of tolerance of submersion and up to 99% of seeds were viable in each treatment regime. The effect of submersion on germination was not obvious at 25oC until the submersion was prolonged for 180 days, while at 30oC the eventual germination rate of X. sibiricum, even after submergence for only one day, was significantly improved. The speed of germination was also consistently accelerated by prolonged periods of submersion. The proportion of seeds that germinated in all treatments combined was less that 56% due to seed dimorphism, thereby providing a seed bank. We conclude that the interaction between long-term winter flooding and high temperature in summer is the major reason that X. sibiricum was able to occupy the newly exposed drawdown area in the absence of competition. These findings provided further insight into how germination strategy and reservoir water-management regime contributed to this dramatic species outbreak.
The study addressed different biological aspects of Juniperus oxycedrus subsp. macrocarpa, including female cone production, biometric analysis of mature cones and seeds, and their viability. The results indicate that the proportion of aborted female cones was very high. In the four populations studied, cone diameter and weight and seed length and weight were closely related. The distribution of numbers of seeds with and without embryos in mature cones was examined, as well as the frequency of numbers of seeds with embryos. The general trend in all populations was three seeds per mature cone, of which only one or two showed embryos. Tetrazolium tests showed the percentage of viable seeds to be lower.
Artificial hybridization experiments revealed a relatively high degree of crossability between Pinus sylvestris and P. mugo, ranging between 0.15 and 0.98. Differences between P. sylvestris x P. mugo and reciprocal hybrids of the species were observed. Hybrid seeds of P. sylvestris x P. mugo shared higher germination potential than seeds from selling, controlled intraspecific crossing, and open pollination. The corresponding value in reciprocal combination was slightly lower. The hybrid nature of the seeds obtained was shown by restriction analysis of chloroplast DNA (cp DNA) using the trnV-trnH/Hinf I primer-enzyme combination.
It was proposed previously that passive dispersal by migratory aquatic birds explain the widespread distribution of many wetland organisms. Several experimental studies have shown that many widespread wetland plant species can be readily dispersed within the guts of Anatidae. However, it is unclear whether plants with a more restricted distribution are able to disperse via waterbirds. This paper addresses the dispersal ability and germination ecology of the little-known Hungarian milkvetch Astragalus contortuplicatus, which occurs on banks of continental rivers and has a limited and unpredictable distribution. To test whether limited capacity for endozoochory by waterfowl could explain the sporadic appearance of this species, we force-fed ten captive mallards (Anas platyrhynchos) with 100 milkvetch seeds each. Droppings were collected for up to 45 h after feeding. Intact and viable seeds were found in the droppings of each mallard, and altogether 24.7% of seeds fed were recovered intact. The proportion of retrieved seeds that germinated (27.0%) was significantly higher than that of untreated control seeds (0.5%), but significantly lower than that of mechanically scarified seeds (96.0%). Retrieved seeds that germinated developed into healthy mature plants. Given the average flight velocity of mallards, seeds of A. contortuplicatus may travel up to 1600 km inside the digestive tract of migrating individuals. Our results suggest that avian vectors may be more important for the dispersal of rare higher plants (especially those with a hard seed-coat) than hitherto considered. Moreover, they suggest that rarity does not necessarily indicate limited dispersal ability, and may instead be explained by specific habitat requirements.
Ustalono możliwości otrzymania mieszańców międzygatunkowych lilii przy zastosowaniu trzech metod zapylenia - tradycyjnego, polegającego na przeniesieniu pyłku z rośliny ojcowskiej na roślinę mateczną oraz dwóch prowadzących do ograniczenia barier krzyżowalności - skrócenia słupka i wycięcia szyjki słupka. Krzyżowania prowadzono między odmianami lilii z grupy Mieszańców Azjatyckich (‘Apeldoorn’, ‘Connecticut King’, ’Dreamland’, ‘Gran Paradiso’, ‘Miss Alice’, ‘Montreux’ ‘Prima’, ‘Roma’) i z grupy Mieszańców Longiflorum (L. longiflorum ‘Snow Queen’, L. longiflorum, L. ×formolongi w latach 1999-2001 w szklarni i na poletkach hali wegetacyjnej AR w Szczecinie. W wyniku przeprowadzonych krzyżowań otrzymano 69 nasion mieszańcowych z wyraźnie widocznym zarodkiem. W większości przypadków były to nasiona pochodzące z zapyleń połączonych z przycinaniem słupka. Próby kiełkowania prowadzono na pożywce MS (Murashige i Skoog 1962) z dodatkiem kazeiny hydrolizowanej (60 g‧dm⁻³ i 200 mg‧dm⁻³), w fitotronie o temperaturze 25°C i natężeniu napromieniowania kwantowego 40 µmol‧m⁻²‧s⁻¹. Spośród 69 nasion mieszańcowych skiełkowało zaledwie jedno, otrzymane ze skrzyżowania ‘Connecticut King’ ×L. longiflorum.
Obiektem badań były mieszańce międzygrupowe lilii Mieszańce Orientalne, Mieszańce Longiflorum, Mieszańce Trąbkowe i Mieszańce Azjatyckie. Określono skuteczność krzyżowania różnych gatunków lilii przy zastosowaniu dwóch różnych sposobów zapyleń oraz wspomagania kiełkowania otrzymanych zarodków w kulturach in vitro. Do udanych krzyżowań zaliczono ‘Montreux’ x ‘Rosato’, ‘Montreux’ x ‘Miami’, ‘Berlin’ x ‘Connecticut King’. L. regale x ‘Apeldoorn’ i ‘Miss Alice’ x ‘La Reve’. Pięć pierwszych mieszańców zawiązało po jednym nasionku. Z krzyżowań między L. regale x ‘Apeldoorn’ i ‘Miss Alice’ x ‘La Reve’ L. regale x ‘Apeldoorn’ i ‘Miss Alice’ x ‘La Reve’ otrzymano po 3 nasiona, między L. regale x ‘Miss Alice’- 2 nasiona, a ze skrzyżowania odmian L. regale x ‘Apeldoorn’ i ‘Miss Alice’ x ‘La Reve’- 5 nasion. W sumie z 452 krzyżowań udało się otrzymać 16 nasion z wykształconymi zarodkami. Wszystkie odznaczały się słabą żywotnością. W kulturach in vitro zainicjowano do wzrostu tylko jedno z zawiązanych nasion ‘Berlin’ x ‘Connecticut King’.
W pracy oceniono żywotność oraz zawartość węglowodanów rozpuszczalnych w nasionach bobiku, grochu, łubinu i soczewicy przechowywanych przez 22 lata w temperaturze -14°C, 0°C oraz w temperaturze pokojowej (około +20°C). Przechowywanie nasion w temperaturze pokojowej powodowało, że nasiona nie kiełkowały, natomiast przechowywane w temperaturze -14°C i 0°C kiełkowały od 86 do 98% w zależności od gatunku. Nasiona przechowywane w temperaturze +20°C miały niższą, w porównaniu do nasion przechowywanych w temperaturze -14°C, 0°C, zawartość oligosacharydów rodziny rafinozy. Podczas przechowywania węglowodany te ulegały degradacji, a proces ten obejmował przede wszystkim werbaskozę i stachiozę.
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